Corrigendum: Development and Improvement of Methods to Disinfect Raw Beef Using Calcium Hydroxide–Ethanol–Lactate-Based Food Disinfectant for Safe Consumption

Kayali, Ahmad Yaman; Ozawa, Jo; Nishibuchi, Mitsuaki

doi:10.3389/fmicb.2021.773509

Cited by 1 publication

(1 citation statement)

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“…Several published studies have developed LAMP assays for detecting stx1 and stx2 genes in food samples [45,46]. In addition, a LAMP STEC detection kit (Eiken Chemical Co., Ltd., Japan) is commercially available [47,48].…”

Section: Pcr-based Toxin Detectionmentioning

confidence: 99%

Recent Advancements in Technologies to Detect Enterohaemorrhagic Escherichia coli Shiga Toxins

Kim

Lee

Park

et al. 2023

J. Microbiol. Biotechnol.

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Shiga toxin (Stxs)-producing enterohaemorrhagic Escherichia coli (EHEC) and Shigella dysenteriae serotype 1 are major causative agents of severe bloody diarrhea (known as hemorrhagic colitis) and hemolytic uremic syndrome (HUS) associated with extraintestinal complications such as acute renal failure and neurologic impairment in infected patients under 9 years of age. Extreme nephrotoxicity of Stxs in HUS patients is associated with severe outcomes, highlighting the need to develop technologies to detect low levels of the toxin in environmental or food samples. Currently, the conventional polymerase chain reaction (PCR) or immunoassay is the most broadly used assay to detect the toxin. However, these assays are laborious, time-consuming, and costly. More recently, numerous studies have described novel, highly sensitive, and portable methods for detecting Stxs from EHEC. To contextualize newly emerging Stxs detection methods, we briefly explain the basic principles of these methods, including lateral flow assays, optical detection, and electrical detection. We subsequently describe existing and newly emerging rapid detection technologies to identify and measure Stxs.

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