Correlative radioimaging and mass spectrometry imaging: a powerful combination to study¹⁴C-graphene oxidein vivobiodistribution

Abstract

Research on Graphene Based Nanomaterials has flourished in the last decade due their unique properties and emerging socio-economic impact. In the context of their potential exploitation for biomedical applications, there...

One-year post-exposure assessment of 14C-few-layer graphene biodistribution in mice: single versus repeated intratracheal administration

“…Our group recently used 14 Clabeled GO to develop a tandem imaging technique combining digital autoradiography (β-imager) with mass spectrometry imaging (MSI), hence providing high resolution and quantitative assessment of its distribution in vivo. 30 In 2013, a graphitized material was labelled with 14 C to assess and measure its uptake and excretion in Daphnia magna. 31 A year later, Czarny et al conducted a study where carbon nanotubes (CNTs) were labelled with 14 C, revealing their translocation across the airblood barrier to distant organs.…”

Environmental and Health Impacts of Graphene and Other Two-Dimensional Materials: A Graphene Flagship Perspective

“…39 Importantly, correlative radioimaging and mass spectrometry imaging (MSI) was recently applied to study biodistribution of 14 C-GO (Figure 19). 390,391 Following intravenous administration in mice, 14 C-GO distribution was thus quantified by radioimaging on tissue slices, whereas on the same slices, MSI provided a highly resolved distribution map of the nanomaterial. Quantitative assessment showed greater amounts of GO in the liver than in the lungs, spleen, and kidneys.…”

“…(C) MSI analysis of the same lung section from mice exposed to 50 μg and 75 μg of 14/12 C-GO with a spatial resolution of 25 (inset C.1 and C.2) and 100 μm. Molecular images of GO were represented using the overlay of maps (purple) obtained for m / z 72 (blue) and 74 (red) ions . Reproduced in part with permission under a Creative Commons 3.0 Unported License from Cazier, H.; Malgorn, C.; Georgin, D.; Fresneau, N.; Beau, F.; Kostarelos, K.; Bussy, C.; Campidelli, S.; Pinault, M.; Mayne-L’Hermite, M.; Taran, F.; Junot, C.; Fenaille, F.; Sallustrau, A.; Colsch, B. Correlative Radioimaging and Mass Spectrometry Imaging: A Powerful Combination to Study 14 C-Graphene Oxide In Vivo Biodistribution.…”

Molecular Imaging, Radiochemistry, and Environmental Pollutants

“…After only 72 h in a container with sediment mixed with 14 C-labeled few-layer graphene, radioactivity was found in the gut and liver of the loaches. In a very recent proof-of-principle study, autoradiography and mass spectrometry imaging were used to visualize 14 C-labeled GO particles (50–400 nm, 1 nm thick) in mouse tissue sections 15 min after intravenous injection ( 39 ). The study validated mass spectrometry imaging as an efficient tool for the determination of the microscopic distribution of GOs.…”