Correlative fluorescence and atomic force microscopy to advance the bio-physical characterisation of co-culture of living cells

Moura, Catarina Costa; Miranda, Adelaide; Oreffo, Richard O.C.; Beule, Pieter De

doi:10.1016/j.bbrc.2020.06.037

Cited by 4 publications

(3 citation statements)

References 26 publications

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“…a especially in the biological sciences field, where it allows the tagging of intracellular molecules and cellular components with high specificity, and their observation inside cells in a minimally invasive manner using non-destructive wavelengths of light in the visible spectrum. 49 AFM has been combined with confocal laser scanning microscopy, [50][51][52][53][54][55][56][57] fast 58 and differential spinning disks, 59,60 and volumetric light sheet microscopy, [61][62][63] as well as some specialised fluorescence characterisation techniques, e.g. Förster Resonance Energy Transfer (FRET), 64 Fluorescence Lifetime Imaging (FLIM), 65 and Fluorescence Correlation Spectroscopy (FCS).…”

Section: Introductionmentioning

confidence: 99%

How did correlative atomic force microscopy and super-resolution microscopy evolve in the quest for unravelling enigmas in biology?

et al. 2021

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Section: Introductionmentioning

confidence: 99%

How did correlative atomic force microscopy and super-resolution microscopy evolve in the quest for unravelling enigmas in biology?

et al. 2021

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“…The commonly used techniques to provide 2-D (surface) or 3-D (without internal structure) visualizations of micro-structures are summarized in Table 1 [9] [14]. The methods are the Scanning Tunneling Microscopes (STM) [26], the Scanning Electron Microscopes (SEM) [27][28] (it has Backscattered Electron (BSE) mode [28]), the Optical Microscopes (OM) [14], the Scanning Probe Microscopes (SPM) [26] (Subtype: Atomic Force Microscope (AFM) [29]), and Confocal Laser Scanning Microscopes (CLSM) [30]. Scanning Tunneling Microscopy (STM) is a type of electron microscopy.…”

Section: Two or Three-dimensional Methods Without Internal Structurementioning

confidence: 99%

Possibilities of porous-structure representation – an overview

Szűcs

Vehovszky

2021

Acta Tech. Jaurinensis

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Porous media can be found in all areas of scientific life, such as medicine, civil engineering, material science, fluid dynamics. Computing has achieved high efficiency and computational capacity – so far. However, three-dimensional Computational Fluid Dynamics (CFD) simulations of microstructure remain significant challenges. Pore-scale simulations can help understand the physical processes and determine macroscopic parameters such as the high-frequency limit of dynamic tortuosity, viscous, and thermal characteristic lengths. Independent of whether the computational problem is two or three-dimensional, the geometry as input parameter must be prepared. For this reason, geometry representation methods play a crucial role in the analysis at the pore-scale, especially in numerical simulations. In this article, an insight into microstructures’ visualization capabilities is provided essentially for CFD simulations.

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“…Finally, as previously outlined, there are various benefits and drawbacks of AFM vs other imaging modalities. However, the combination of AFM with advanced optical microscopy is particularly promising [ 49 , 50 , 51 , 52 , 53 ]. It would be highly interesting to use AFM in conjunction with fluorescence or Raman imaging modalities simultaneously to observe spectroscopic signatures associated with gelation on the same field of view as that of the AFM image.…”

Section: Future Outlookmentioning

confidence: 99%

Atomic Force Microscopy of Phytosterol Based Edible Oleogels

Matheson,

Koutsos,

Euston

et al. 2023

Gels

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This work reviews the use of atomic force microscopy (AFM) as a tool to investigate oleogels of edible triglyceride oils. Specific attention is given to those oleogels based on phytosterols and their esters, a class of material the authors have studied extensively. This work consists of a summary of the role of AFM in imaging edible oleogels, including the processing and preparation steps required to obtain high-quality AFM images of them. Finally, there is a comparison between AFM and other techniques that may be used to obtain structural information from oleogel samples. The aim of this review is to provide a useful introduction and summary of the technique for researchers in the fields of gels and food sciences looking to perform AFM measurements on edible oleogels.

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Correlative fluorescence and atomic force microscopy to advance the bio-physical characterisation of co-culture of living cells

Cited by 4 publications

References 26 publications

How did correlative atomic force microscopy and super-resolution microscopy evolve in the quest for unravelling enigmas in biology?

How did correlative atomic force microscopy and super-resolution microscopy evolve in the quest for unravelling enigmas in biology?

Possibilities of porous-structure representation – an overview

Atomic Force Microscopy of Phytosterol Based Edible Oleogels

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