Correlative analysis of the expression of IL‑10 and Ki‑67 in human cervical cancer and cervical intraepithelial neoplasias and human papillomavirus infection

Abstract

This study investigated the expression of IL-10 and Ki-67 in human cervical cancer and cervical intraepithelial neoplasia (CIN) and the correlation with human papillomavirus infection. A total of 110 patients with cervical lesions undergoing surgical treatment in Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine from 2016 to 2017 were selected. Those patients included 36 cases of cervical cancer and 74 cases of CIN. At the same time, 30 cases of chronic cervicitis were selected as the control group. RT-qPCR was used to detect the expression of IL-10 and Ki-67 in cervical tissue. PCR was used to detect HPV infection in cervical tissue. The expression levels of IL-10 and Ki-67 in the cervical cancer and CIN groups were higher than those in the control group. Moreover, the expression levels of IL-10 and Ki-67 in the cervical cancer and CIN II–III groups were higher than those in the CIN I group (P<0.05). In addition, the expression levels of IL-10 and Ki-67 in the cervical cancer group were significantly higher than those in the CIN II–III group. Furthermore, the expression levels of IL-10 and Ki-67 were positively correlated with HPV infection (r=0.783 or 0.712, P<0.05). Finally, the expression levels of IL-10 and Ki-67, and HPV infection in the cervical lesions studied were significantly different. Therefore, combined detection of IL-10, Ki-67 and HPV infection can improve the diagnosis of CIN and early cervical cancer.

Preliminary Study on the Expression of Testin, p16 and Ki-67 in the Cervical Intraepithelial Neoplasia

“…Ki-67 is a cellular marker of proliferation, detected in the non-G0 phase of the cell cycle [24]. In normal squamous cervical epithelium, Ki-67 is present only in basal and parabasal layers.…”

The analysis of HPV integration sites based on nanopore sequencing and the profiling changes along the course of photodynamic therapy

“…Glutathione peroxidase 3 (GPX3) was the member of the glutathione peroxidase family of selenoproteins, GPX3 promoter methylation was one of the main causes of downregulated GPX gene expression in cervical cancer and acted as a predictive biomarker for lymph node metastasis and prognosis in cervical cancer [ 45 ]. In cervical cancer tissues, the expression levels of IL10 and Ki-67 increased accordingly when the HPV infection rate increased, so the detection of IL10, KI67 expression and HPV infection rate can provide reference for the diagnosis of cervical cancer [ 46 ]. Thrombocythemia, high PLR (platelet to lymphocyte ratio) and IL-6 overexpression correlated with cervical cancer progression and metastasis, they may play an important role as molecular markers of survival and may be considered as potential predictors of cervical cancer prognosis [ 47 ].…”