Correlations of platelet-leukocyte aggregates with P-selectin S290N and P-selectin glycoprotein ligand-1 M62I genetic polymorphisms in patients with acute ischemic stroke

Li, Tao; Wang, Changfu; Li, Linyun; Mei, Bing; Hu, Xiaohui

doi:10.1016/j.jns.2016.05.046

Cited by 15 publications

(7 citation statements)

References 29 publications

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“…Although platelet activation markers were significantly higher in both the stroke subgroups than the control group, they were found to be insignificant when the two subgroups were compared among themselves. These results are in keeping with the reports of Oberheiden et al and Li Tao et al for cardioembolic stroke 37 , 38 . Similarly, Marquardt et al, demonstrated that both PNA and PMA are significantly higher in acute stroke patients than in control.…”

Section: Discussionsupporting

confidence: 93%

Platelet function suggests cardioembolic aetiology in cryptogenic stroke

Dev

Dash

Pathak

2023

Sci Rep

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Platelet-monocyte (PMA) and platelet-neutrophil aggregations (PNA) play critical roles in the evolution of acute ischemic stroke (AIS). The present study investigates the mechanistic basis of platelet responsiveness in cryptogenic stroke compared with cardioembolic stroke. Platelet from 16 subjects, each from cryptogenic and cardioembolic stroke groups and 18 age-matched healthy controls were subjected to different investigations. Compared to healthy controls, platelet-monocyte and platelet-neutrophil interactions were significantly elevated in cryptogenic (2.7 and 2.1 times) and cardioembolic stroke (3.9 and 2.4 times). P-selectin expression on platelet surface was 1.89 and 2.59 times higher in cryptogenic and cardioembolic strokes, respectively, compared to healthy control. Cell population with [Ca2+i] in either stroke group was significantly outnumbered (by 83% and 72%, respectively, in cryptogenic and cardioembolic stroke) in comparison to healthy controls. Noteworthy, TEG experiment revealed that the cryptogenic stroke exhibited significant decline in Reaction Time (R) and amplitude of 20 mm (K) (by 32% and 33%, respectively) while thrombin burst (α-angle) was augmented by 12%, which reflected substantial boost in thrombus formation in cryptogenic stroke. Although TEG analysis reveals a state of hypercoagulability in patients with cryptogenic stroke. However, platelets from both stroke subtypes switch to a ‘hyperactive’ phenotype.

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Section: Discussionsupporting

confidence: 93%

Platelet function suggests cardioembolic aetiology in cryptogenic stroke

Dev

Dash

Pathak

2023

Sci Rep

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“…In particular, increased binding of platelets to monocytes and neutrophils were observed (82–84), while only few reports of platelet-lymphocyte interactions exist (85). An increase in intermediate CD14 ++ CD16 + monocytes and their binding to platelets 7 days post percutaneous coronary intervention after myocardial infarction is further associated with an increased risk for ischemic stroke besides other adverse cardiovascular events (86).…”

Section: Platelet-leukocyte Interactions In Pathologic Conditionsmentioning

confidence: 99%

Measuring and interpreting platelet-leukocyte aggregates

et al. 2018

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Platelets, besides their specialised role in haemostasis and atherothrombosis, actively modulate innate and adaptive immune responses with crucial roles in immune surveillance, inflammation and host defence during infection. An important prerequisite for platelet-mediated changes of immune functions involves direct engagement with different types of leukocytes. Indeed, increased platelet-leukocyte aggregates (PLAs) within the circulation and/or locally at the site of inflammation represent markers of many thrombo-inflammatory diseases, such as cardiovascular diseases, acute lung injury, renal and cerebral inflammation. Therefore, measurement of PLAs could provide an attractive and easily accessible prognostic and/or diagnostic tool for many diseases. To measure PLAs in different (patho-)physiological settings in human and animal models flow cytometric and microscopic approaches have been applied. These techniques represent complementary tools to study different aspects relating to the involvement of leukocyte subtypes and molecules, as well as location of PLAs within tissues, dynamics of their interactions and/or dynamic changes in leukocyte and platelet behaviour. This review summarises various approaches to measure and interpret PLAs and discusses potential experimental factors influencing platelet binding to leukocytes. Furthermore, we summarise insights gained from studies regarding the underlying mechanism of platelet-leukocyte interactions and discuss implications of these interactions in health and disease.

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“…P-selectin (SELP) is mainly expressed on the surface of activated endothelial cells and platelets [11,26]. Its expression upregulation is correlated with the pathogenesis of various diseases, including atherosclerosis [65], thrombosis [66], diabetes [67] and cancer [14,24,25,28,68]. Because P-selectin has been shown to promote the formation of cancer metastases by facilitating the adhesion and migration of tumor cells [24,25], this molecule has emerged as a potential therapeutic strategy for the treatment of various conditions.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics Analyzes of Selectins Polymorphisms Reveal New Potential Cancer Biomarkers

Rabi¹,

Valente²,

Teixeira³

et al. 2023

Preprint

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Selectins are responsible for the early stages of cell migration as they control cell adhesion. They make the microenvironment permissive for metastatic events by promoting the activation of other cell adhesion molecules (CAMs). We employed several robust bioinformatics tools to evaluate gene sequence; single nucleotide polymorphisms (SNPs) in intronic and UTR regions; and missense SNPs with amino acid change in L-selectin (SELL), E-selectin (SELE), P-selectin (SELP) and PSGL-1 (SELPLG). We demonstrated that gene polymorphisms rs2229569, rs1131498, rs4987360, rs4987301 and rs2205849; polymorphisms rs3917777, rs2205894 and rs2205893 of SELP gene; rs7138370, rs7300972 and rs2228315 variants of SELPLG gene; and rs1534904 and rs5368 polymorphisms of SELE gene may produce important alterations in the DNA structure and consequent alterations in the morphology and function of the corresponding proteins. These Selectin polymorphic variants deserve further investigation in cancer patients, as they may become useful clinical markers for risk determination, diagnostic and prognostic biomarkers or even targets for targeted therapies.

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Correlations of platelet-leukocyte aggregates with P-selectin S290N and P-selectin glycoprotein ligand-1 M62I genetic polymorphisms in patients with acute ischemic stroke

Cited by 15 publications

References 29 publications

Platelet function suggests cardioembolic aetiology in cryptogenic stroke

Platelet function suggests cardioembolic aetiology in cryptogenic stroke

Measuring and interpreting platelet-leukocyte aggregates

Bioinformatics Analyzes of Selectins Polymorphisms Reveal New Potential Cancer Biomarkers

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