Correlations of Ki-67 and PCNA to DNA ploidy, S-phase fraction and survival in uveal melanoma

Karlsson, Matts; Boeryd, B; Carstensen, John; Frånlund, B.; Gustafsson, Bertil; Kågedal, Bertil; Sun, Xiao‐Feng; Wingren, Sten

doi:10.1016/0959-8049(95)00562-5

Cited by 38 publications

(26 citation statements)

References 23 publications

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“…However, our results using PCNA immunohistochemistry staining showed little variation between the five cell lines; therefore, no ranking structure could be determined. Immunostaining with monoclonal antibody against PCNA will give the staining of all cells in any phase of mitosis [16]. We utilized established cell lines that are already assumed to be replicating at a high level, which our results confirmed.…”

Section: Discussionsupporting

confidence: 68%

Cell Proliferation Profile of Five Human Uveal Melanoma Cell Lines of Different Metastatic Potential

et al. 2004

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Objective: The aim of this study was to establish a proliferation profile of uveal melanoma cell lines, using different methods, and to compare it with their previously determined metastatic potential (MP). Methods: Four human uveal melanoma and one transformed human uveal melanocytic cell line were ranked according to proliferation profiles. The proliferation profiles of the cell lines were compared to their MPs, which were previously determined from an immunosuppressed rabbit model. Results: Ranking of the cell lines using pulse labeling with tritiated thymidine was similar to the MP of the cell lines. Conclusion: The correlation between the proliferative rate of the uveal melanoma cell lines and their previously determined MP resulted in the proposal of a new classification scheme: high proliferation/high MP, low proliferation/low MP, and high proliferation/no MP. High proliferative capacity of a cell line did not necessarily confer MP; therefore, further cellular functions/adaptations must be required for tumor cell dissemination, survival, and growth at a metastatic site.

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Section: Discussionsupporting

confidence: 68%

Cell Proliferation Profile of Five Human Uveal Melanoma Cell Lines of Different Metastatic Potential

et al. 2004

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“…In several larger series of breast cancers with longer follow-ups, investigators did not find a significant relationship (Gasparini et al, 1992(Gasparini et al, , 1994Thomas et al, 1993). In 165 prostatic carcinomas (Visakorpi, 1992) and 79 uveal melanomas (Karlsson et al, 1996), no prognostic value of PCNA was observed either. In our large series, we did not correlate PCNA with prognosis.…”

Section: Discussionmentioning

confidence: 95%

Proliferating cell nuclear antigen (PCNA) in relation to ras, c-erbB-2, p53, clinico-pathological variables and prognosis in colorectal adenocarcinoma

et al. 1996

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Proliferating cell nuclear antigen (PCNA) expression was studied by immunohistochemistry on paraffin-embedded sections of 293 primary colorectal adenocarcinomas and 56 corresponding lymph node metastases. PCNA-positive expression was detected in <25% of tumour cells in 172 (59%) cases and in >25% in I 2 I (4 I YO) cases. PCNA accumulation was related to over-expression of c-erbB-2 and p53 and tended to be increased in cases with ras over-expression. PCNA expression was identical in primary and corresponding metastases. No significant relationship was observed between PCNA expression and prognosis and other clinico-pathological variables, including grade of differentiation, growth pattern, Dukes' stage, site, age or sex. We conclude that PCNA expression may be related to alterations of oncoproteins but that PCNA itself could not provide additional information for the development of metastasis and prognosis in colorectal adenocarcinoma.o 1996 Wiley-Liss, Inc.Proliferating cell nuclear antigen (PCNA) is a 36-kDa nuclear protein and a marker of cell proliferation, which progressively accumulates in late GI and early S phases and disappears at the end of mitosis. It acts as an auxiliary protein of the DNA polymerase delta essential for the processive activity of this enzyme in cells entering S phase (Lane, 1989). The ras gene family consists of H-ras, K-ras and N-ras genes and codes for 21-kDa proteins, which are located on the inner surface of the plasma membrane (Barbacid, 1987). The proteins possess intrinsic GTPase activity and participate in the transduction of signals across the cell membrane (Barbacid, 1987). The c-erbB-2 gene encodes a transmembrane protein with strong sequence homology to the epidermal growth factor receptor; thus, this protein has been postulated to be a receptor for some growth factors (Maguire and Greene, 1989). p53 is a nuclear phosphoprotein and regulates cellular growth and differentiation (Lane, 1989). Since these oncoproteins are implicated in cellular proliferation, they may be related to PCNA. However, there is little information on the correlation of PCNA with these oncoproteins in colorectal tumours. The results concerning the relationship between PCNA expression ;and clinico-pathological parameters or prognosis of colorectal cancers are conflicting (Al-Sheneber et al., 1993;Linden et al., 1993;Mayer et al., 1993;Lazaris et al., 1994;Teixeira et al., 1994;Willett et al., 1994). Therefore, in this large series of colorectal adenocarcinomas, we have analysed the relationship between PCNA expression and ras, c-erbB-2, p53, clinicopathological parameters as well as survival using immunohistochemistry. PATIENTS AND METHODS Patients and pathological dataTwo hundred and ninety-three patients with primary colorec-1.al adenocarcinoma diagnosed at the Department of Pathology, University Hospital of Linkoping, were consecutively collected from 1972 to 1986. Among them, regional lymph nodes with metastasis from 56 patients were obtained. No patient had received pre-operative radiotherapy or ...

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“…Expression of Ki-67 (MIB 1) helped to identify melanomas of high metastatic potential [20,21] and was associated with poor survival [18,21]. Expression of PCNA was useful in prediction of locoregional and distant metastases [14,15].…”

Section: Proliferative Parametersmentioning

confidence: 98%

“…Some results point to markers of proliferative activity [14][15][16][17][18][19][20][21] and expression of p53 protein [14,17,[22][23][24][25][26] as variables of prognostic value. In the present study, prognostic value of common clinical and histological parameters in cutaneous malignant melanoma together with proliferative activity and some tumor markers have been evaluated.…”

Section: Introductionmentioning

confidence: 99%

Prognostic evaluation of cutaneous malignant melanoma: A clinicopathologic and immunohistochemical study

et al. 1999

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Correlations of Ki-67 and PCNA to DNA ploidy, S-phase fraction and survival in uveal melanoma

Cited by 38 publications

References 23 publications

Cell Proliferation Profile of Five Human Uveal Melanoma Cell Lines of Different Metastatic Potential

Cell Proliferation Profile of Five Human Uveal Melanoma Cell Lines of Different Metastatic Potential

Proliferating cell nuclear antigen (PCNA) in relation to ras, c-erbB-2, p53, clinico-pathological variables and prognosis in colorectal adenocarcinoma

Prognostic evaluation of cutaneous malignant melanoma: A clinicopathologic and immunohistochemical study

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