Correlations of <i>in vitro</i> and <i>in vivo</i> degradation tests on electrospun poly‐<scp>DL</scp>‐lactide‐poly(ethylene glycol) fibers

He, Shuhui; Li, Xiaohong; Yang, Ye; Jia, Guoqing; Zou, Jie

doi:10.1002/app.36436

Cited by 8 publications

(9 citation statements)

References 34 publications

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“…Likely, because of the high diffusion of water molecules in a hydrogel, a bulk degradation should be expected. However, to predict material behavior in applications, a thorough analysis of material properties during the degradation is a prerequisite . Furthermore, it is of interest to investigate whether in the presence of enzymes, resembling the in vivo situation, the degradation mechanism stays the same.…”

Section: Introductionmentioning

confidence: 99%

“…However, to predict material behavior in applications, a thorough analysis of material properties during the degradation is a prerequisite. [15,16] Furthermore, it is of interest to investigate whether in the presence of enzymes, [17] resembling the in vivo situation, the degradation mechanism stays the same. It would somehow be a trivial finding to observe surface degradation when the enzyme is too large to enter the polymer mesh, which indeed has been shown especially for hydrophobic polymers.…”

Section: Introductionmentioning

confidence: 99%

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Enzymatic action as switch of bulk to surface degradation of clicked gelatin‐based networks

Piluso

Lendlein

Neffe

2016

Polymers for Advanced Techs

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Polymer degradation occurs under physiological conditions in vitro and in vivo, especially when bonds susceptible to hydrolysis are present in the polymer. Understanding of the degradation mechanism, changes of material properties over time, and overall rate of degradation is a necessary prerequisite for the knowledge‐based design of polymers with applications in biomedicine. Here, hydrolytic degradation studies of gelatin‐based networks synthesized by copper‐catalyzed azide‐alkyne cycloaddition reaction are reported, which were performed with or without addition of an enzyme. In all cases, networks with a stilbene as crosslinker proofed to be more resistant to degradation than when an octyl diazide was used. Without addition of an enzyme, the rate of degradation was ruled by the crosslinking density of the network and proceeded via a bulk degradation mechanism. Addition of Clostridium histolyticum collagenase resulted in a much enhanced rate of degradation, which furthermore occurred via surface erosion. The mesh size of the hydrogels (>7 nm) was in all cases larger than the hydrodynamic radius of the enzyme (4.5 nm) so that even in very hydrophilic networks with large mesh size enzymes may be used to induce a fast surface degradation mechanism. This observation is of general interest when designing hydrogels to be applied in the presence of enzymes, as the degradation mechanism and material performance are closely interlinked. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enzymatic action as switch of bulk to surface degradation of clicked gelatin‐based networks

Piluso

Lendlein

Neffe

2016

Polymers for Advanced Techs

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“…In tissue engineering, for instance, scaffold resorption should match the rate of tissue formation, so that the overall structural integrity of the system is maintained. Too rapid a degradation rate would not only jeopardize the mechanical support afforded by the scaffold, but would also cause accumulation of the acidic matter that could induce inflammation of the surrounding tissue .…”

Section: Introductionmentioning

confidence: 99%

In vitro degradation of PLLA/nHA composite scaffolds

et al. 2013

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Porous Poly‐l‐lactide (PLLA) scaffolds and PLLA/nanohydroxyapatite (nHA) composite scaffolds with interconnected pore networks and a porosity of over 90% were fabricated with lyophilization techniques. In this study, the degradation behavior of PLLA and PLLA/nHA composite scaffolds is investigated over 8 weeks in phosphate buffer solution at 37°C. Thermal analysis using differential scanning calorimetry (DSC) showed that the percent crystallinity of all the samples increased by approximately 10%, which represents a considerable increase in the glass transition temperature. The melting range enthalpy of the scaffolds did not change to lower temperatures as would be expected. The spectroscopic analysis performed by Fourier transform infrared spectroscopy suggested that nHA particles should not appreciably affect the absorbance pattern when evenly mixed with the PLLA. This is consistent with the analysis of the scaffold microstructure and morphology with scanning electron microscopy, which drew a low content of nHA with no significant effect on solvent crystallization or pore structure. The compressive modulus and the yield strength of the scaffolds were investigated in conjunction with the study of their degradation rates. In comparison with the mechanical properties of the PLLA scaffolds, which remained largely unchanged, those of the PLLA/nHA composite scaffolds decreased as the degradation progressed. POLYM. ENG. SCI., 54:2571–2578, 2014. © 2013 Society of Plastics Engineers

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“…However, in contrast to the polymer fi lms which maintained their dimensions or were slightly extended due to swelling, the polymer meshes shrank massively in buffer, which can be attributed to a relaxation of the polymer chains which experience high alignment and orientation through the electrospinning process. [ 8,23 ] Shrinkage was observed to higher extents for polymers with longer PLA chain lengths and is very undesirable for an application as barrier device as an unrestricted coverage of the peritoneal site of injury is not ensured. Due to their inherent porousness, meshes showed way higher swelling or water uptake than the massive fi lms with higher water uptake for the linear triblock copolymer than for the star-shaped copolymers although the star-shaped copolymers showed a better anchorage of the PEG (compare NMR after eight weeks) due to the more connecting points of water-soluble PEG to lipophilic PLA chains.…”

Section: Surface Examination and Swellingmentioning

confidence: 99%

“…

electrospun meshes [ 7,8 ] regarding their suitability for biomedical applications and drug delivery. Rising attention is given to biodegradable and biocompatible starshaped block copolymers with PEG core as they bring along additional benefi cial properties such as providing multiple functional groups and by representing a versatile tool for building structured polymer networks like hydrogels.

…”

mentioning

confidence: 99%

Application of Linear and Branched Poly(Ethylene Glycol)‐Poly(Lactide) Block Copolymers for the Preparation of Films and Solution Electrospun Meshes

Keßler

Groll

Teßmar

2015

Macromolecular Bioscience

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Poly(ethylene glycol)-poly(lactide) (PEG-PLA) block copolymers are processed to solvent cast films and solution electrospun meshes. The effect of polymer composition, architecture, and number of anchoring points for the plasticizer on swelling, degradation, and mechanical properties of these films and meshes is investigated as potential barrier device for the prevention of peritoneal adhesions. As a result, adequate properties are achieved for the massive films with a longer retention of the plasticizer PEG for star-shaped block copolymers than for the linear triblock copolymers and consequently more endurable mechanical properties during degradation. For electrospun meshes fabricated using the same polymers, similar trends are observed, but with an earlier start of fragmentation and lower tensile strengths. To overcome the poor mechanical strengths and an occurring shrinkage during incubation, which may impair the coverage of the wound, further adaptions of the meshes and the fabrication process are necessary.

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Correlations of in vitro and in vivo degradation tests on electrospun poly‐DL‐lactide‐poly(ethylene glycol) fibers

Cited by 8 publications

References 34 publications

Enzymatic action as switch of bulk to surface degradation of clicked gelatin‐based networks

Enzymatic action as switch of bulk to surface degradation of clicked gelatin‐based networks

In vitro degradation of PLLA/nHA composite scaffolds

Application of Linear and Branched Poly(Ethylene Glycol)‐Poly(Lactide) Block Copolymers for the Preparation of Films and Solution Electrospun Meshes

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