Correlations between total protein, lysozyme, immunoglobulins, amylase, and albumin in stimulated whole saliva during daytime

Abstract

The correlations between salivary proteins and the daytime variations are not known. The present study investigated the within-subject variation of correlations and concentrations between lysozyme, IgA, IgG, IgM, albumin, amylase, and total protein in stimulated whole saliva of healthy adults in the course of a 12-h period. After several practise sessions, unstimulated and stimulated whole saliva samples were collected five times daily (at 8 a.m., 11 a.m., 2 p.m., 5 p.m., and 8 p.m.) from 30 healthy university students. Flow rate and total protein concentration were used as covariates, and gender as a between-subject factor in the MANOVA analysis. After this adjustment, there was significant within-subject variation in salivary IgA (P < 0.001), albumin (P < 0.01), amylase (P < 0.05), and total protein (P < 0.001) concentrations. Total protein correlated significantly with amylase albumin and IgA through different samplings. In addition, IgG correlated with albumin and lysozyme in the course of 12 h. On the whole, the correlations between variables remained stable during repeated samplings. In addition, rankings of subjects for the variables tended to be maintained across different samplings (P < 0.001). However, the observed within-subject variations in salivary IgA, albumin, amylase, and total protein concentrations suggest that these proteins are subject to short-term variation.

Determinants of salivary evening alpha-amylase in a large sample free of psychopathology

“…In accordance to previous studies we did not find an association between gender on sAA levels (Ben-Aryeh et al, 1986;Nater et al, 2007;Rantonen and Meurman, 2000). Most previous studies failed to find an effect of age on sAA levels (Aguirre et al, 1987;BenAryeh et al, 1986;Salvolini et al, 1999).…”

“…Regarding demographic factors, no sex differences in basal sAA have been found (Nater et al, 2007;Rantonen and Meurman, 2000;Rohleder et al, 2004). Furthermore, basal sAA seemed rather stable across the life span (Aguirre et al, 1987;Ben-Aryeh et al, 1986;Salvolini et al, 1999).…”

“…Furthermore, most studies measured sAA levels only once per subject at a single time-point, mostly between 9:00 h and 12:00 h (Aguirre et al, 1987;Ben-Aryeh et al, 1986;Dutta et al, 1992;Goi et al, 2007;Granger et al, 2007a;Nagaya and Okuno, 1993). Only some studies collected more samples over the day to assess the diurnal pattern (Nater et al, 2007;Rantonen and Meurman, 2000). The use of late evening sAA levels yields the opportunities to study sAA at its peak level due to the diurnal profile.…”

Principles of demineralization: Modern strategies for the isolation of organic frameworks

“…Minerals in saliva present from food are dissolved by the carbonic acids. In addition, carbonic acid quickly and easily converts itself to carbon dioxide and water under these conditions (Rantonen and Meurman, 2000). When this happens, the dissolved mineral ions precipitate out as solid mineral ions again, but not always as the original mineral molecules.…”

Salivary IgA response to prolonged exercise in a hot environment in trained cyclists

“…whether s-IgA was reported as absolute concentration, secretion rate, ratio to total saliva protein, ratio to saliva albumin or ratio to saliva osmolality). Saliva flow rate has been reported to significantly affect oral health via the flushing of microorganisms and their products into the gut and by providing a continuous supply of both non-immune and immune factors in the mouth (Rantonen and Meurman 2000;Tenovuo 1998). Individuals who suffer from drymouth syndrome (xerostomia) suffer an increased incidence of URTI (Fox et al 1985).…”