Correlations between the Phylogenetic Relationship of 14 Tulasnella Strains and Their Promotion Effect on Dendrobium crepidatum Protocorm

Zhao, Jian; Li, Zhenjian; Wang, Siyu; Fu, Yang; Li, Lubin; Liu, Lei

doi:10.3390/horticulturae8121213

Cited by 2 publications

(1 citation statement)

References 70 publications

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“…On the other hand, the development of new tissues in plants inoculated with the form genus Rhizoctonia coincides with that reported by Hossain et al [41] and Zhao et al [42]. However, no root pelotons were observed in the present study.…”

Section: Symptoms Induced By Form Genus Rhizoctoniasupporting

confidence: 93%

Evaluation of Tulasnella and Ceratobasidium as Biocontrol Agents of Fusarium Wilt on Vanilla planifolia

Manrique-Barros,

Flanagan,

Ramírez-Bejarano

et al. 2023

Agronomy

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Fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. vanillae (Fov), is a disease that results in significant losses in commercial vanilla production. The genera Ceratobasidium (Ceratobasidiaceae) and Tulasnella (Tulasnellaceae), which are often reported as mutualistic symbionts in orchids, belong to the form genus Rhizoctonia, a paraphyletic group of fungi with potential for pathogen biocontrol. We assayed the antagonistic properties of the form genus Rhizoctonia from the roots of neotropical orchids: two Tulasnella spp. isolates (Bv3 and Er1) and one Ceratobasidium sp. (Er19). In a dual culture, we found that form genus Rhizoctonia isolates can generate a biocontrol effect against Fusarium through the mechanisms of antibiosis and competition for space and nutrients. On histological observations, orchid root endophytes also demonstrated potential for mutualistic symbiosis development by establishing themselves on the surface and within the root tissue of Vanilla planifolia accessions multiplied in vitro (NSF021 and NSF092). However, in plant assays, the form genus Rhizoctonia isolates did not reduce symptom expression or disease development due to infection by Fov in the host. These results contribute to the knowledge of the interactions between tropical orchids and their microbiota and demonstrate the need for multidisciplinary studies for the implementation of integrated management strategies for Fusarium disease in commercial systems.

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Section: Symptoms Induced By Form Genus Rhizoctoniasupporting

confidence: 93%

Evaluation of Tulasnella and Ceratobasidium as Biocontrol Agents of Fusarium Wilt on Vanilla planifolia

Manrique-Barros,

Flanagan,

Ramírez-Bejarano

et al. 2023

Agronomy

View full text Add to dashboard Cite

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Morphogenesis Changes in Protocorm Development during Symbiotic Seed Germination of Dendrobium chrysotoxum (Orchidaceae) with Its Mycobiont, Tulasnella sp.

et al. 2023

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The endangered epiphytic orchid, Dendrobium chrysotoxum, is known for its ornamental and medicinal uses. However, knowledge of this orchid’s symbiotic seed germination, protocorm anatomy, and developmental morphology is completely unknown. In this study, we investigated the process of protocorm development of D. chrysotoxum during symbiotic germination using anatomical technologies and scanning electron microscopy. There are six development stages that were morphologically and anatomically defined during symbiotic seed germination. The embryo transformed into a protocorm at stage two, and a protrusion developed from the top of the protocorm at stage three and elongated to form the cotyledon at stage four. The stem apical meristem (SAM) was initiated at stage three and well developed at stage four. The first leaf and the root appeared at stages five and six, respectively. The hyphae entered through the micropylar end of the seed at stage one and then invaded the protocorm through rhizoids when rhizoids formed. Invading fungal hyphae colonized the inner cortex at the base of protocorms, formed pelotons, and were digested by host cells later. We conclude that protocorm development is programmed by the embryo, which determines the structure and function of the protocorm. The two developmental zones in a polarized D. chrysotoxum embryo include the smaller cells zone, which forms the cotyledon and a shoot apical meristem at the apical end, and the larger cells zone, which forms the mycorrhiza to house the symbiont at the basal end. These results will provide important insights for further research on the mechanisms underlying orchid-fungi symbiosis and enhance the understanding of orchid evolution.

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Correlations between the Phylogenetic Relationship of 14 Tulasnella Strains and Their Promotion Effect on Dendrobium crepidatum Protocorm

Cited by 2 publications

References 70 publications

Evaluation of Tulasnella and Ceratobasidium as Biocontrol Agents of Fusarium Wilt on Vanilla planifolia

Evaluation of Tulasnella and Ceratobasidium as Biocontrol Agents of Fusarium Wilt on Vanilla planifolia

Morphogenesis Changes in Protocorm Development during Symbiotic Seed Germination of Dendrobium chrysotoxum (Orchidaceae) with Its Mycobiont, Tulasnella sp.

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