Correlations between HPLC and NMR properties of some selected alkyl bonded phases

Abstract

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Retention mechanisms in reversed-phase liquid chromatography. Stationary-phase bonding density and partitioning

“…Many workers have attempted to correlate solute retention with the carbon content of RP stationary phases. Claessens et al (11) and Scott and Kucera (12) linearly correlated k'and percent carbon and corrected retention volume and percent carbon, respectively, for various commercial octadecyl and octyl columns. Shaikh and Tomaszewski (13) found a linear relationship between k' and carbon loading for seven commercial octadecyl packings whereas Engelhardt and Ahr (14) noted linearity between log A'and percent carbon for four commercial octadecyl phases.…”

Deuterium Nuclear Magnetic Resonance Spectroscopy as a Probe For Reversed-Phase Liquid Chromatographic Bonded-Phase Solvation. 3. Tetrahydrofuran and Water Binary Systems

“…Previous studies − have demonstrated the usefulness of solution-state nuclear magnetic resonance (NMR) spectroscopy in the investigation of molecular interactions in RPLC systems. However, many of these studies were performed with uncommon solvents or only encompassed a narrow range of binary compositions. ,,− , Previous work in our laboratory 2-4 has indicated that, with an appropriate wetting procedure for the stationary phase, the entire binary composition range for hydro-organic mixtures of acetonitrile and methanol becomes accessible for study via solution-state deuterium ( 2 H) NMR.…”