Correlations and co-localizations of Hsp70 with XPA, XPG in human bronchial epithelia cells exposed to benzo[a]pyrene

Yang, Jin; Liu, Xiaoyong; Niu, Piye; Zou, Yue; Duan, Yanying

doi:10.1016/j.tox.2009.09.001

Cited by 20 publications

(11 citation statements)

References 40 publications

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“…The regulation of HSP70 gene in response to BaP remains unclear. For example, high concentration of BaP (10 µM) suppress the transcription of HSP70 gene in human endothelial cell [50], and BaP (1 µM) up-regulate HPS70 mRNA in bronchial cells, suggesting a potential role of HSP70 in the NER DNA repair [51].…”

Section: Discussionmentioning

confidence: 99%

Metallothionein mRNA induction is correlated with the decrease of DNA strand breaks in cadmium exposed zebra mussels

Vincent-Hubert¹,

Châtel²,

Gourlay-Francé³

2014

Mutation Research/Genetic Toxicology and Environmental Mutagene

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We have previously shown that cadmium (Cd) and benzo[a]pyrene (BaP) induced early DNA damages in zebra mussels, and that the level of DNA strand breaks (SB) returned to a basal level after 3 days of exposure to Cd. The aim of the present study was to go further in the mechanisms of Cd and BaP detoxification. For that purpose, expression of genes encoding for metallothionein (MT), aryl hydrocarbon receptor (AHR), P-gp, catalase, glutathione S-transferase and heat shock protein 70 (HSP70) proteins have been measured using RT-qPCR. Data reported here show that Cd is a strong inducer of MT and HSP70 genes, and that BaP is a strong inducer of P-gp and AHR genes. Exposure to Cd and BaP resulted in moderate changes in antioxidant enzymes mRNA. Since the increase of MT mRNA occurred when the DNA SB level returned to its basal level, we can suggest that MT is implicated in cadmium detoxification.

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Section: Discussionmentioning

confidence: 99%

Metallothionein mRNA induction is correlated with the decrease of DNA strand breaks in cadmium exposed zebra mussels

Vincent-Hubert¹,

Châtel²,

Gourlay-Francé³

2014

Mutation Research/Genetic Toxicology and Environmental Mutagene

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“…On the cellular level, human bronchial epithelial 16HBE cells treated with BaP (as a source of reactive BPDE ) displayed greater expression of the NER proteins XPA and XPG and the heat shock protein Hsp70. Subcellular analysis with confocal microscopy evidenced nuclear colocalization of Hsp70 with XPA and XPG after BaP treatment, suggesting that Hsp70 has a role in the cellular DNA repair response [173]. Accordingly, (+/−) -anti - BPDE induces chromosome instability and centromere amplification in lung cells [174].…”

Section: Drug-induced Dna Destabilization: Cellular Consequencesmentioning

confidence: 99%

DNA‐Destabilizing Agents as an Alternative Approach for Targeting DNA: Mechanisms of Action and Cellular Consequences

Lenglet

David-Cordonnier

2010

Journal of Nucleic Acids

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DNA targeting drugs represent a large proportion of the actual anticancer drug pharmacopeia, both in terms of drug brands and prescription volumes. Small DNA-interacting molecules share the ability of certain proteins to change the DNA helix's overall organization and geometrical orientation via tilt, roll, twist, slip, and flip effects. In this ocean of DNA-interacting compounds, most stabilize both DNA strands and very few display helix-destabilizing properties. These types of DNA-destabilizing effect are observed with certain mono- or bis-intercalators and DNA alkylating agents (some of which have been or are being developed as cancer drugs). The formation of locally destabilized DNA portions could interfere with protein/DNA recognition and potentially affect several crucial cellular processes, such as DNA repair, replication, and transcription. The present paper describes the molecular basis of DNA destabilization, the cellular impact on protein recognition, and DNA repair processes and the latter's relationships with antitumour efficacy.

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“…DnaK, the bacterial homolog of HspA1A, maintains the properly folded state of DNA repair proteins and participates in NER (Zou et al 1998). One of our previous studies also identified an increased co-localization between HspA1A and the NER proteins XPA and XPG in cell nucleus after exposure to Benzo[a]pyrene (BaP) (Yang et al 2009). However, the underlying mechanism of how HspA1A facilitates DNA repair remains elusive.…”

Section: Introductionmentioning

confidence: 99%

HspA1A facilitates DNA repair in human bronchial epithelial cells exposed to Benzo[a]pyrene and interacts with casein kinase 2

Duan

Huang

Yang

et al. 2014

Cell Stress and Chaperones

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Benzo[a]pyrene (BaP) is a ubiquitously distributed environmental pollutant that induces deoxyribonucleic acid (DNA) damage. The inducible heat shock protein (HspA1A) can function as a molecular chaperone; however, its role in DNA repair remains largely unknown. In the present study, human bronchial epithelial cells (16HBE) stably transfected with plasmids carrying HspA1A gene or shRNAs against HspA1A were treated with BaP. DNA damage levels of the cells were evaluated by comet assay. Results suggest that HspA1A could protect cells against DNA damage and facilitate the decrease of DNA damage levels during the first 2 h of DNA repair. DNA repair capacity (DRC) of Benzo(a)pyrene diol epoxide (BPDE)-DNA adducts was evaluated by host cell reactivation assay in the stable 16HBE cells transfected with luciferase reporter vector PCMVluc pretreated with BPDE. Compared with control cells, cells overexpressing HspA1A showed higher DRC (p < 0.01 at 10 μM BPDE and p < 0.05 at 20 μM BPDE, respectively), while knockdown of HspA1A inhibited DNA repair (p < 0.05 at 10 μM BPDE). Moreover, casein kinase 2 (CK2) was shown to interact with HspA1A by mass spectrometry and co-immunoprecipitation assays. The two proteins were co-localized in the cell nucleus and perinuclear region during DNA repair, and were identified by confocal laser scanning microscope. In addition, cells overexpressing HspA1A showed an increased CK2 activity after BaP treatment compared with control cells (p < 0.01). Our results suggest that HspA1A facilitates DNA repair after BaP treatment. HspA1A also interacts with CK2 and enhances the kinase activities of CK2 during DNA repair.

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Correlations and co-localizations of Hsp70 with XPA, XPG in human bronchial epithelia cells exposed to benzo[a]pyrene

Cited by 20 publications

References 40 publications

Metallothionein mRNA induction is correlated with the decrease of DNA strand breaks in cadmium exposed zebra mussels

Metallothionein mRNA induction is correlated with the decrease of DNA strand breaks in cadmium exposed zebra mussels

DNA‐Destabilizing Agents as an Alternative Approach for Targeting DNA: Mechanisms of Action and Cellular Consequences

HspA1A facilitates DNA repair in human bronchial epithelial cells exposed to Benzo[a]pyrene and interacts with casein kinase 2

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