In view of the technical limitations of nitrogen removal of leachate treatment project in a domestic waste comprehensive disposal site, a Two-Stage Sequencing Batch Biological Contact Oxidation Reactor (I/II-SBBCOR for short) was proposed, and the comparative and orthogonal experiment of the I/II-SBBCOR was carried out, in order to optimize the treatment process and operation parameters. The experiment showed that: Nitrogen removal process of I/II-SBBCOR is remarkably better than its prototype, Hydraulic Retention Time (HRT for short), Sequencing Batch Cycle (SBC for short), and Dissolved Oxygen (DO for short), are the significant factors affecting the nitrogen removal efficiency of the I/II-SBBCOR, and the significance order from highest to lowest was: HRT, SBC, DO; with HRT decreasing from 12d/6d to 2d/1d, the removal rate of ammonia nitrogen (NH4+-N for short) and total nitrogen (TN for short) present a downward trend; with SBC decreasing from 12h to 1h and DO increasing from1.0/1.5mg/L to 2.5/3.0 mg/L, the removal efficiency of NH4+-N increased, while that of TN increased first and then decreased; and the optimal combination of operating parameters of the I/II-SBBCOR are: HRT = 6d/3d, SBC = 6 h, DO = 2.0 /2.5mg/L.