Correlation study on serum miR-222-3p and glucose and lipid metabolism in patients with polycystic ovary syndrome

Abstract

Objective microRNAs (miRNAs) play pivotal roles in polycystic ovary syndrome (PCOS), an endocrine and metabolic disorder that commonly occurs in women of childbearing age. This paper aimed to measure miR-222-3p expression in sera of PCOS patients and to explore its clinical value on PCOS diagnosis and prediction of diabetic and cardiovascular complications. Methods Totally 111 PCOS patients and 94 healthy people were recruited and assigned to the overweight (ow) group and non-overweight (non-ow) group, followed by determination of serum miR-222-3p expression. The diagnostic efficiency of miR-222-3p on PCOS ow and non-ow patients was analyzed. Correlations between miR-222-3p and glycolipid metabolic indicators and diabetic and cardiovascular complications in PCOS were analyzed. The downstream target of miR-222-3p was predicted and their binding relationship was verified. The correlation between PGC-1α and miR-222-3p was analyzed. Results miR-222-3p was highly-expressed in PCOS patients (p < 0.001), in especially PCOS ow patients. The area under the curve (AUC) of miR-222-3p diagnosing PCOS non-ow patients was 0.9474 and cut-off value was 1.290 (89.06% sensitivity, 98.11% specificity), indicating that non-ow people with serum miR-222-3p > 1.290 could basically be diagnosed with PCOS. AUC of miR-222-3p diagnosing PCOS ow patients was 0.9647 and cut-off value was 2.425 (85.11% sensitivity, 100% specificity), suggesting that ow people with serum miR-222-3p > 2.425 could basically be diagnosed with PCOS. miR-222-3p was positively-correlated with fasting plasma glucose (FPG), fasting insulin (FINS), homeostatic model assessment–insulin resistance (HOMA-IR), and low-density lipoprotein cholesterol (LDL-C) and negatively-correlated with high-density lipoprotein cholesterol (HDL-C). miR-222-3p was independently-correlated with diabetic and cardiovascular complications in PCOS (p < 0.05). High expression of miR-222-3p predicted high risks of diabetic and cardiovascular complications in PCOS. miR-222-3p targeted PGC-1α and was negatively-correlated with PGC-1α (r = − 0.2851, p = 0.0224; r = − 0.3151, p = 0.0310). Conclusion High expression of miR-222-3p assisted PCOS diagnosis and predicted increased risks of diabetic and cardiovascular complications. miR-222-3p targeted PGC-1α and was negatively-correlated with PGC-1α.

Role of Increased miR-222-3p Expression in Peripheral Blood and Wound Marginal Tissues of Type 2 Diabetes Mellitus Patients with Diabetic Foot Ulcer

“…Furthermore, high miR-222-3p expression exhibits a positive relation to FPG in patients with GDM, T2DM, and PCOS. 8–10 Consistently, in T2DM patients treated with metformin, reduced miR-222 in the PB positively correlates with the reduction of FPG and HbA1c. 6 Nevertheless, in patients suffering T2DM and PCOS, high miR-222-3p expressions report lower HDL-C. 10 All these conformed to our study results.…”

“…MiR-222-3p is a highly conserved miRNA mainly expressed in bladder, prostate, lung, ovary, adipose tissue, thyroid, bronchus, islet, skin, and other tissues. 5 Studies have found an increasing miR-222-3p expression in the peripheral blood (PB) of patients suffering type 2 diabetes mellitus (T2DM), 6 obesity, 7 polycystic ovary syndrome, 8 and gestational diabetes mellitus. 9 According to a cohort follow-up study, miR-222-3p overexpression in the mononuclear cells of PB raises the risk of T2DM.…”

Investigating material basis and molecular mechanism of Qing Cuo formula in the treatment of acne based on animal experiments, UPLC-LTQ-Orbitrap-MS and network pharmacology

“…2011 ). Cryptotanshinone lessens the combination of androgen with the androgen-receptors present in the sebaceous glands and inhibits the sebaceous glands from secreting acne caused by the sebaceous glands of the hair follicle (Wang N 2015 ). In summary, constituents absorbed into blood of QCF contain antibacterial, anti-inflammatory and anti-androgen components.…”