Correlation of von Willebrand factor gene polymorphism and coronary heart disease

Abstract

To characterize von Willebrand factor (vWF) gene polymorphisms at site A1381T and the correlation of plasma vWF levels with coronary heart disease, the vWF genotypes at site A1381T were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in patients diagnosed with coronary heart disease and normal controls (n=110 per group), and plasma vWF levels were measured by enzyme‑linked immunosorbent assay. The results showed that the plasma vWF levels were higher in the experimental group than in the control group and had no association with gender (t=11.69, p<0.05). In the experimental group, the plasma vWF levels were higher in the patients with the AG genotype than in those with the GG genotype (p<0.05). In the control group, the plasma vWF levels of the subjects with blood type O were significantly lower than those of the individuals with other blood types (p<0.05). In the experimental group, all blood types had significantly higher plasma vWF levels than the control group and the difference was significant among different blood types (p<0.05). In summary, vWF gene polymorphisms at site A1381T were not associated with coronary heart disease, but plasma vWF levels were influenced by vWF gene polymorphisms at site A1381T, blood type and coronary heart disease.

Guggulsterone regulates the function and expression of P-glycoprotein in rat brain microvessel endothelial cells

“…In the presence of 1mM egtazic acid (to inhibit Ca 2+ -ATPase), 0.5mM ouabain (to inhibit the Na + /K + -ATPase), and 10mM azide (to inhibit mitochondrial ATPase), the ATPase activity measured for the P-glycoprotein containing membranes can be attributed to P-glycoprotein which represents 20% of the total membrane protein, the basal ATPase activity determined in the absence of any added agent was about 84.7 ± 5.13 μmol• min -1 •g -1 protein. The dose-response curve of guggulsterone showed a stimulatory characteristic, which was similar to previous results (Nabekura et al, 2008;Xu et al, 2012.). However, verapamil revealed a biphasic characteristic.…”

“…The ATPase activity of P-glycoprotein was stimulated by guggulsterone, as well as verapamil, known substrate of P-glycoprotein. Our previous study also showed that guggulsterone could stimulate P-glycoprotein ATPase activity in a dose-dependent manner using purified reconstituted P-glycoprotein membrane (Xu et al, 2012). Although plasma membranes isolated from multidrug-resistant cell lines contains lower percent of P-glycoprotein as fraction of total membrane protein than that of purified reconstituted P-glycoprotein, there is no significant difference of the effect of guggulsterone on P-glycoprotein ATPase activity between them.…”

“…This gum resin has been used for centuries in Ayurvedic medicine to treat obesity, arthritis, and hyperlipidemia (Sinal and Gonzalez, 2002;Urizar and Moore, 2003). In our previous research, we found that guggulsterone could inhibit P-glycoprotein-mediated multidrug resistance in P-glycoprotein over-expressed human cancer cell lines (Xu et al, 2009;Xu et al, 2011;Xu et al, 2012). In the present report, we investigated the effects of guggulsterone on the P-glycoprotein function and expression in rat brain microvessel endothelial cells (rBMECs).…”

A meta‐analysis of the association of adiponectin gene polymorphisms with coronary heart disease in Chinese Han population

“…We considered all case-control studies that examined the association of the adiponectin polymorphisms with CHD in Chinese Han populations. [8][9][10][11][12][13][14][15][16][17][18] Sources included MEDLINE, Chinese National Knowledge Infrastructure (CNKI) and Chinese WanFang database (up to January 2011). The search strategy was based on the combination of 'adiponectin gene', 'apM1', 'polymorphism', 'coronary heart disease' and 'China/Chinese'.…”

“…Eleven studies [8][9][10][11][12][13][14][15][16][17][18] probing the association between adiponectin gene polymorphism and CHD were identified. They all met the eligible criteria.…”