Correlation of uric acid, urea, ammonia and creatinine of seminal plasma with semen parameters and fertilization rate of infertile couples

Abstract

Background: Ammonia, urea, uric acid, and creatinine are the major non-protein nitrogenous compounds (NPNs). It is reported that the concentration of NPNs in the seminal plasma of normal and infertile individuals is different and sperm is affected by NPNs. Objectives: The aim of this study was to determine the quantities of ammonia, urea, uric acid, and creatinine in seminal plasma and the correlation of these compounds with the fertilization rate after an intracytoplasmic sperm injection (ICSI). Methods: The levels of ammonia, urea, uric acid, and creatinine were determined in seminal plasma collected from 50 men. The ammonia and urea were determined by L-glutamate dehydrogenase and diacetyl monoxime method, respectively. Uric acid and creatinine were detected by enzymatic method and Jaffe reaction, respectively. The fertilization was evaluated around 16–18 hours post-ICSI on the appearance of 2 pronuclei and 2 polar bodies. The fertilization rate was calculated by the number of fertilized oocytes per the number of oocytes injected. Results: There was a significant negative correlation between seminal ammonia and sperm motility (P<0.05). Urea and creatinine had a negative correlation with respect to the sperm count (P<0.05). In addition, there was an inverse correlation between urea and uric acid of seminal plasma and sperm morphology (P<0.05). A significant negative correlation was found between seminal uric acid and urea with respect to the percentage of the fertilization rate (P<0.05). Conclusions: The findings of the present study showed that urea and uric acid in seminal plasma have a negative impact on the fertilization rate.

Bacteria May Deteriorate Progressive Motility of Bovine Spermatozoa and Biochemical Parameters of Seminal Plasma

“…On one hand, UA may scavenge hydroxyl radicals. On the other hand, UA converts to U which, according to several studies (Allahkarami et al, 2017;Halo Jr. et al, 2018), may be related to a decreased sperm motility, which is in conflict with our results. The correlation analysis revealed significant (P<0.01) and positive (0.651) correlation between PROG and U.…”

Associations of Sperm Mitochondrial DNA Copy Number with Concentrations of Urinary Cadmium and Selenium

“…However, we do not know any biologically plausible explanation for the associations of urinary speci c gravity and creatinine with sperm mtDNAcn. A negative association was observed between seminal creatinine concentrations and sperm count in patients with intracytoplasmic sperm injection [52], but it is unknown whether urinary creatinine concentration re ects seminal creatinine concentration, and thus the nding of the previous study [52] does not explain the associations observed in this study.…”

Sexually transmitted doping: The impact of urine contamination of semen

“…Accordingly, in this study, we used creatinine as a tracing metric for urine volume from concurrently measured concentration of creatinine in urine and seminal plasma. Few previous studies have reported measuring creatinine in semen samples [15][16][17] but none measured urine creatinine concurrently to quantify urine content of semen samples. One study of eight men after resection of the bladder base displayed pathological leakage of urine into the ejaculate 17 whereas the present study investigated men with normal bladder base sphincter function.…”