Correlation of the surface chemistries of polymer bioactive coatings, with their biological performances

West, R. H.; Paul, A. J.; Hibbert, S.; Cahalan, Patrick T.; Cahalan, Linda; Verhoeven, Mwgm Tiny; Hendriks, M.; Fouache, Benedicte

doi:10.1007/bf00120408

Cited by 12 publications

(6 citation statements)

References 3 publications

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“…The successful immobilization of a molecule is not enough to impart to the substrate surface all the properties expected from the molecular structure of the coating molecule, a more detailed tuning of the property-controlling-parameter(s) is mandatory. This is well-known in applications involving specific interactions, where the control of the structure of the immobilized molecule is a key issue, as nicely shown by heparin-coated surfaces . The present results indicate the importance of these issues in the prevention of nonspecific interactions and have obvious implications for process design and quality control.…”

Section: Discussionsupporting

confidence: 67%

Surface Studies on a Model Cell-Resistant System

Morra

Cassinelli

1999

Langmuir

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A model system involving a polyanionic alginic acid (AA) overlayer ionically immobilized on a polycationic polyethyleneimine underlayer, in turn linked to plasma oxidized polystyrene, was used to investigate the relationship between surface structure properties and resistance to cell adhesion. X-ray Photoelectron Spectroscopy (XPS) and water contact angle measurement were used to characterize the surfaces and to compare the results with data on resistance to adhesion of L-929 fibroblasts. A overlayer model was used to account for the XPS results in terms of fractional coverage by AA. Results show the strong effect of the surface density of AA chains on resistance to cell adhesion and underline the inadequacy of simple relationships between wettability by water and cell adhesion.

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Section: Discussionsupporting

confidence: 67%

Surface Studies on a Model Cell-Resistant System

Morra

Cassinelli

1999

Langmuir

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“…This method expresses bioactivity with respect to the ability of the surface to deactivate thrombin and is described in West. 10 Heparinized surfaces are rinsed extensively in 0.5 M sodium chloride (NaCl) to remove any loose heparin. The surface is then rinsed with deionized water and exposed to a solution of 1 IU/mL of AT III in Hepes buffer for 15 min with gentle mixing.…”

Section: Methodsmentioning

confidence: 99%

In vitro assessment of blood compatibility: Residual and dynamic markers of cellular activation

et al. 2011

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The blood compatibility of materials and surfaces used for medical device fabrication is a crucial factor in their function and effectiveness. Expansion of device use into more sensitive and longer term applications warrants increasingly detailed evaluations of blood compatibility that reach beyond the customary measures mandated by regulatory requirements. A panel of tests that assess both deposition on the surface and activation of circulating blood in contact with the surface has been developed. Specifically, the ability of a surface to modulate the biological response of blood is assessed by measuring: (1) dynamic thrombin generation; (2) surface-bound thrombin activity after exposure to blood; (3) activation of monocytes, polymorphonuclear leukocytes, lymphocytes, and platelets; (4) activation of complement; and (5) adherent monocytes, polymorphonuclear leukocytes, lymphocytes, and platelets on blood-contacting surfaces. The tests were used to evaluate surfaces modified with immobilized heparin (Ension's proprietary bioactive surface) and demonstrated that the modified surfaces reduced platelet activation, leukocyte activation, and complement activation in flowing human blood. Perfusion of the surfaces with human platelet-rich plasma showed that the immobilized heparin surfaces also reduce both dynamic thrombin levels in the circulating plasma and residual thrombin generated at the material surface.

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“…Immobilization of HA via formation of an ionic complex with polycationic surfaces has been widely studied, especially in the formation of alternate polycationic−polyanionic layers, an approach that is enjoying much popularity today. Sequential adsorption of oppositely charged colloids was reported in 1966, and sequential adsorption of polycationic poly(ethylenimine) (PEI) and polyanionic dextran sulfate and heparin has been widely used for many years in the biomedical industry, for instance, in the Carmeda process for heparin coating . Polyelectrolyte multilayers involving HA have been widely investigated.…”

Section: Surface Modification By Hamentioning

confidence: 99%

“…Sequential adsorption of oppositely charged colloids was reported in 1966, 98 and sequential adsorption of polycationic poly(ethylenimine) (PEI) and polyanionic dextran sulfate and heparin has been widely used for many years in the biomedical industry, for instance, in the Carmeda process for heparin coating. 99 Polyelectrolyte multilayers involving HA have been widely investigated.…”

Section: Surface Modification By Hamentioning

confidence: 99%

Engineering of Biomaterials Surfaces by Hyaluronan

2005

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This review addresses the area of study that defines the field of surface modification of biomedical materials and devices by hyaluronan (HA), as related to the exploitation of HA biological properties. To provide a comprehensive view of the subject matter, initial sections give a quick introduction to basic information on HA-protein and HA-cell interactions, together with some discussion on the bioactive role of HA in wound healing and related phenomena. This is followed by a description of current theories that correlate HA properties to its molecular structure in aqueous media, underlying how HA molecular details are crucial for its biological interaction and role. Finally, existing approaches to surface modification by HA are reviewed, stressing the need for HA-surface engineering founded on the knowledge and control of the surface-linked HA molecular conformation at the solid/aqueous interface.

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Correlation of the surface chemistries of polymer bioactive coatings, with their biological performances

Cited by 12 publications

References 3 publications

Surface Studies on a Model Cell-Resistant System

Surface Studies on a Model Cell-Resistant System

In vitro assessment of blood compatibility: Residual and dynamic markers of cellular activation

Engineering of Biomaterials Surfaces by Hyaluronan

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