Correlation of structure to antitumor activities of five derivatives of a β-glucan from Poria cocos sclerotium

Wang, Yifeng; Zhang, Lina; Li, Yunqiao; Hou, Xiaohua; Zeng, Fanbo

doi:10.1016/j.carres.2004.08.003

Cited by 157 publications

(83 citation statements)

References 29 publications

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“…Nowadays, to improve the utilization ratio of Poria cocos sclerotia, several methods have been adopted to modify pachyman. [9][10][11][12] In some cases, Poria cocos sclerotia is ground into powder and added to flour or rice powder directly to make cakes (http://www.cnncn.net/zlk/24/binglei75.htm). However, the universal method to treat pachyman is through chemical reactions, which can improve water solubility and bioactivities of pachyman by resecting the b-(1-6) branched chain, leaving only b-(1-3) linear dextran, or introducing hydrophilic groups into pachyman, or improving some other characteristics.…”

Section: Introductionmentioning

confidence: 99%

“…However, the universal method to treat pachyman is through chemical reactions, which can improve water solubility and bioactivities of pachyman by resecting the b-(1-6) branched chain, leaving only b-(1-3) linear dextran, or introducing hydrophilic groups into pachyman, or improving some other characteristics. 7,9,10,[12][13][14][15] For example, carboxymethyl pachyman (CMP) with antitumor and inhibitory tumor cell growth bioactivities 12,16,17 was produced by heating pachyman with chloroacetic acid in alkaline solution. 9 Correspondingly, sulfated pachyman, which can inhibit the growth of tumor cells, was prepared by treating pachyman with chlorosulfuric acid in pyridine solution.…”

Section: Introductionmentioning

confidence: 99%

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Preparation of bioactive water-soluble pachyman hydrolyzed from sclerotial polysaccharides of Poria cocos by hydrolase

Bian

Xie

Chen

2010

Polym J

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Water-soluble pachyman (WSP) was prepared by enzymic hydrolysis of pachyman extracted from Poria cocos sclerotium using a 0.5 mol l À1 NaOH solution at the optimized conditions of 55 1C, pH 4, 4% pachyman, 2 U ml À1 hydrolase, and a hydrolysis time of 6 h. Two WSP fractions, WSP-1 and WSP-2, were prepared using SephadexG-100 to separate WSP. Both fractions were of the b-(1-3)-D-dextran structure and were only composed of glucose with no nucleic acid and protein. The composition was analyzed by ultraviolet spectroscopy and infrared spectroscopy, Congo Red reaction analysis and monosaccharide composition analysis. The effects of WSP (M w 1.75Â10 5 ), WSP-1 (M w 1.86Â10 6 ) and WSP-2 (M w 3.58Â10 4 ) on the proliferation of S180 tumor cells in vivo and in vitro were studied. The inhibitory ratios of the three tested WSPs on the proliferation of S180 tumor cells at 0.5 lg ml À1 in vitro were found to be 4.0 ± 0.6, 2.2 ± 0.1 and 2.6 ± 0.1%, respectively, and the inhibitory ratio of carboxymethyl pachyman (CMP), which was used as a control, was À0.3±0.4%. WSP, WSP-1 and WSP-2 also exhibited significant inhibition on S180 tumor cells in vivo at a dose level of 200 mg kg À1 day À1 , with their inhibition ratios found to be 43.94, 41.57 and 39.81%, respectively. Furthermore, the three tested WSPs had no obvious effect on the weight of mice, whereas the administration of CMP resulted in a decrease in mice weight at the tested concentration.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preparation of bioactive water-soluble pachyman hydrolyzed from sclerotial polysaccharides of Poria cocos by hydrolase

Bian

Xie

Chen

2010

Polym J

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“…Furthermore, the introduction of sulfated groups to polysaccharides alters its physicochemical characterization and chain conformation (Wang, Li, & Chen, 2009). It has also been reported (Wang, Zhang, Li, Hou, & Zeng, 2004) that relatively expanded and stiff chain conformation of sulfated polysaccharides can improve anticancer effects. Figura 8.…”

Section: Discussionmentioning

confidence: 90%

Effect of sulfation on the antioxidant properties and in vitro cell proliferation characteristics of polysaccharides isolated from corn bran

Zhou

et al. 2016

CyTA - Journal of Food

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Polysaccharides were extracted from corn bran (CBPs) and then modified by a sulfating reaction to obtain sulfated corn bran polysaccharides (SCBPs). Synthesized sulfated polysaccharides were located in the equatorial bonds of monosaccharide residues. A more orderly and smooth surface of SCBPs was observed. Single factor experiment results showed 2.0 g of aminosulfonic acid, 60 mL of N-dimethylformamide and 2.5 h of reaction time were the optimum conditions for SCBPs preparation. Phenolic content of SCBPs significantly decreased with increasing concentration of aminosulfonic acid, while it increased with increasing DMF concentration and reaction time. SCBPs had higher DPPH, ABTS and iron chelating capacity under the optimum conditions. SCBPs significantly inhibited the proliferation of A549 and HepG2 cell lines, which was associated with the upregulation at the mRNA expression level of pro-apoptotic genes CASP3, CASP8, CASP9 and reduced expression of p53, gene Bcl-2 and gene iNOS.

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“…The tightly sealed bags were then immersed into 30 mL medium in a conical flask, and the whole system was incubated in a water bath at 37°C with a shaking speed of 100 rpm. At predetermined time intervals (2,4,6,8,10,12,24,48,72, and 96 hours), 0.5 mL aliquots of the incubation medium were withdrawn and replaced by an equal volume of fresh solution. The aliquots were quantitatively measured at 490 nm using a ultraviolet-visible light spectrometer.…”

Section: In Vitro Drug Releasementioning

confidence: 99%

“…3,4 The carbohydrate chain generally consists of β-d-(1→3)-linked glucose units with a small number of β-(1→6)-linked branches and has a molecular weight ranging from 64.6 kDa to 4,360 kDa. [5][6][7][8] Studies have shown PHY to have pharmaceutical value, as it has diuretic, 9 mitogenic, complement-activating, anti-inflammatory, 10 and immunoactive properties. PHY also has a number of beneficial physicochemical properties that make it suitable for widespread applications in drug delivery systems.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of optimum conditions for pachyman encapsulated in poly(D,L-lactic acid) nanospheres by response surface methodology and results of a related in vitro study

Zheng

Luo

et al. 2016

IJN

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This study aimed to optimize the preparation conditions of pachyman (PHY)-loaded poly( d,l -lactic acid) (PLA) (PHYP) nanospheres by response surface methodology, explore their characteristics, and assess their effects on splenic lymphocytes. Double emulsion solvent evaporation was used to synthesize PHYP nanospheres, and the optimal preparation conditions were identified as a concentration of poloxamer 188 (F68) (w/v) of 0.33%, a concentration of PLA of 30 mg/mL, and a ratio of PLA to drug (w/w) of 10.25:1 required to reach the highest encapsulation efficiency, which was calculated to be 59.10%. PHYP had a spherical shape with a smooth surface and uniform size and an evident effect of sustained release and relative stability. Splenic lymphocytes are crucial and multifunctional cells in the immune system, and their immunological properties could be enhanced significantly by PHYP treatment. This study confirmed that PHY encapsulated in PLA nanospheres had comparatively steady properties and exerted obvious immune enhancement.

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Correlation of structure to antitumor activities of five derivatives of a β-glucan from Poria cocos sclerotium

Cited by 157 publications

References 29 publications

Preparation of bioactive water-soluble pachyman hydrolyzed from sclerotial polysaccharides of Poria cocos by hydrolase

Preparation of bioactive water-soluble pachyman hydrolyzed from sclerotial polysaccharides of Poria cocos by hydrolase

Effect of sulfation on the antioxidant properties and in vitro cell proliferation characteristics of polysaccharides isolated from corn bran

Evaluation of optimum conditions for pachyman encapsulated in poly(D,L-lactic acid) nanospheres by response surface methodology and results of a related in vitro study

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