Correlation of soil microbiome with crude oil contamination drives detection of hydrocarbon degrading genes which are independent to quantity and type of contaminants

Exploring the diversity and functional profile of microbial communities of Brazilian soils with high salinity and oil contamination

“…According to the COG analysis, there is a significant reduction in most functions from ALW to ALC, this functional difference may have occurred due to the stress promoted by the contaminants in ALC. In agreement with our findings, previous reports demonstrate that high salinity and hydrocarbon concentrations may lead to restructuring in soil microbial functions and metabolic pathways [ 18 , 88 , 93 ].…”

“…Allied to the taxonomic composition of a microbial community, an important aspect of metagenomic studies is the assessment of the contribution of each component of the sample regarding the functional genes. Previous studies have documented a correlation between the identification of functional genes in polluted environments and the adaptation and reshaping of the indigenous microbial community [ 88 , 89 ]. Based on the COG annotations, 22 functional categories were identified in the present study.…”

Response Characteristics of the Community Structure and Metabolic Genes of Oil-Recovery Bacteria after Targeted Activation of Petroleum Hydrocarbon-Degrading Bacteria in Low-Permeability Oil Reservoirs

“…Understanding the biodegradation pathway of alkane hydrocarbons by PHDB was important from the standpoint of biotransformation that the existence of an abundance of hydrocarbon-degrading enzymes was proved after PHDB activation. These petroleum microbes contained oxygenase enzymes that could introduce oxygen into the terminal, nonactivated position of different alkane hydrocarbons in crude oil. , Medium and long-chain alkanes were oxidized by integral-membrane nonheme diiron monooxygenases (alkB) or alternatively by heme iron-containing cytochrome P450 monooxygenases, and P450 of terminal monooxygenase was mainly responsible for the degradation of medium alkanes. alkB and P450 could complement each other.…”