Correlation of SIDT1 with Poor Prognosis and Immune Infiltration in Patients with Non-Small Cell Lung Cancer

Abstract

Purpose To investigate the expression profile of systemic RNA interference deficient-1 transmembrane family member 1 (SIDT1) in patients with non-small cell lung cancer (NSCLC) and assess its prognostic value and immune infiltration. Materials and Methods A tissue microarray (TMA) was purchased containing 60 paired NSCLC tissues and matched para-tumor tissues, and another TMA was constructed comprising 140 NSCLC tissues. Then, immunohistochemical staining and scoring were performed. Finally, we evaluated the expression profiles and prognostic values of SIDT1 in NSCLC. Correlation between SIDT1 gene expression and the immune microenvironment of NSCLC was investigated using the ESTIMATE and CIBERSORT algorithms. Results SIDT1 was mainly detected in the cytoplasm and the cell membrane. SIDT1 expression was significantly higher in tumor tissues than in para-tumor tissues. High expression of SIDT1 was correlated with the NSCLC stage. High SIDT1 expression signals worsening overall survival (OS) in NSCLC patients, especially in stage I. In LUSC, high SIDT1 expression is an independent prognostic factor for worsening OS. Conclusion High SIDT1 expression predicted a low survival rate in LUSC patients, suggesting it may be a potential target for prognostic assessment of NSCLC patients.

Characterization of N-glycosylation and its functional role in SIDT1-Mediated RNA uptake

“…In several diseases, SIDT1 and SIDT2 are implicated. SIDT1 plays a key role in breast, pancreatic, and non–small cell lung cancer ( 7 , 8 , 9 ). Loss of function studies has demonstrated an essential role for SIDT2 in the regulation of glucose metabolism, lipid metabolism, antiviral immunity, skeletal muscle homeostasis, and tumor development ( 5 , 6 , 7 , 8 , 12 , 13 ).…”

“…Mammalian SID-1 transmembrane family member 1 (SIDT1) and member 2 (SIDT2) are the human orthologs of Caenorhabditis elegans systemic RNA interference defective protein 1 (SID-1), which is required for transporting exogenous dsRNA into the cytoplasm and is therefore essential for systemic RNA interference in C. elegans ( 1 , 2 , 3 , 4 ). Previous studies on mammalian SIDT1 and SIDT2 have implicated them in various biological processes such as glucose and lipid metabolism, immune response, and tumorigenesis, including breast, lung, gastrointestinal, pancreatic and non–small cell lung cancers, but the specific mechanisms are unknown ( 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ). In contrast, the role of SIDT1 and SIDT2 in regulating various physiological processes by mediating cellular RNA uptake and intracellular trafficking have been extensively studied, providing significant insights into their functional contributions.…”

Characterization ofN-glycosylation and its Functional Role in SIDT1-Mediated RNA Uptake

“…In several diseases, SIDT1 and SIDT2 are implicated. SIDT1 plays a key role in breast, pancreatic, and non-small cell lung cancer 8,9,11 . Loss of function studies has demonstrated an essential role for SIDT2 in the regulation of glucose metabolism, lipid metabolism, antiviral immunity, skeletal muscle homeostasis, and tumor development [5][6][7][8][9][10] .…”

“…Mammalian SID-1 transmembrane family member 1 (SIDT1) and member 2 (SIDT2) are the human orthologs of Caenorhabditis elegans systemic RNA interference defective protein 1 (SID-1), which is required for transporting exogenous doublestranded RNA (dsRNA) into the cytoplasm and is therefore essential for systemic RNA interference in Caenorhabditis elegans (1)(2)(3)(4). Previous studies on mammalian SIDT1 and SIDT2 have implicated them in various biological processes such as glucose and lipid metabolism, immune response, and tumorigenesis, including breast, lung, gastrointestinal, pancreatic and non-small cell lung cancers (NSCLC), but the specific mechanisms are unknown (5)(6)(7)(8)(9)(10)(11)(12)(13). In contrast, the role of SIDT1 and SIDT2 in regulating various physiological processes by mediating cellular RNA uptake and intracellular trafficking have been extensively studied, providing significant insights into their functional contributions.…”

PIEZO2 promotes cell proliferation and metastasis in colon carcinoma through the SLIT2/ROBO1/VEGFC pathway

“…We evaluated the PIEZO2 expression in colon cancer tissues using the staining intensity and staining area of positive tumor cells in each section. 18 The immunohistochemical score was used to assess the expression of PIEZO2 referred to the protocol described by Tian et al 19 The median score was used as the split point. The group with values higher than or equal to the median was considered the high-expression group, and the the group with values lower than the median was considered the low-expression group.…”