Correlation of serum and synovial leptin concentrations with body condition scores in healthy and osteoarthritic dogs

Abstract

Objective To evaluate the relationship between serum and synovial fluid (SF) leptin concentrations and body condition score (BCS) in healthy and osteoarthritic dogs. Study design Controlled, prospective, clinical study. Animals Nineteen healthy dogs and 29 dogs with osteoarthritis (OA) secondary to cranial cruciate ligament injury. Methods Synovial fluid was obtained from the femorotibial joint under sedation (healthy dogs) or during surgery (OA dogs). Serum and SF leptin and interleukin (IL)‐1β concentrations were measured via enzyme‐linked immunosorbent assay. Dogs were classified as optimal weight (BCS 4–5/9) or overweight (BCS >5/9). Radiographs were scored for OA severity by a radiologist. Owners completed the Liverpool Osteoarthritis in Dogs (LOAD) questionnaire. Results Mean (± SD) SF leptin (4.09 ± 4 ng/mL) was lower than serum leptin (6.88 ± 5.52 ng/mL, P < .0001). Synovial fluid leptin was higher in overweight (5.28 ± 4.21) than in optimal body weight dogs (1.54 ± 1.72 ng/mL, P < .0001). Serum (P < .001) and SF leptin (P = .004) concentrations were associated with BCS. Concentration of SF leptin did not differ between healthy (2.4 ± 2.04 ng/mL) and OA (4.9 ± 4.3 ng/mL, P = .25) dogs. Synovial fluid leptin and LOAD scores were weakly associated (P = .03). No association was detected between SF leptin and radiographic score or IL‐1β (P = .73). Conclusion Serum and SF leptin correlated with BCS in this population. Synovial fluid leptin was weakly associated with LOAD scores but not with radiographic severity of OA or IL‐1β. Clinical significance Serum and SF leptin concentrations do not predict radiographic severity of canine OA but contribute to joint pain and dysfunction.

Assessment of Outcome of Arthroscopic Subtotal Coronoidectomy in Treating Medial Coronoid Disease and Effect of Concurrent Autologous Conditioned Plasma in Dogs Using Force Plate Analysis

“…28 29 Adipokines including leptin are produced by adipose cells and are directly related to body condition scores and the development and progression of osteoarthritis. 30 31…”

“…28,29 Adipokines including leptin are produced by adipose cells and are directly related to body condition scores and the development and progression of osteoarthritis. 30,31 Normalizing GRF based only on body weight may not be sufficient for analysing dogs of different breeds as body configuration may also play a role in differences in GRF. Several studies have reported variations in GRF among different breeds.…”

Associations between early neutering, obesity, outdoor access, trauma and feline degenerative joint disease

“…Circulating levels of leptin, which reflect body fat mass, are increased in overweight cats, dogs and humans, 76 and the proinflammatory role of leptin in the development of DJD has been established in humans 77 and dogs. 73 , 74 , 78 , 79 Although there is a paucity of studies investigating the role of mechanical and biochemical factors in the relationship between obesity and feline DJD, it is possible that these postulations could also apply to cats.…”

Clinical Metrology Instrument in Veterinary Medicine

“…Они представляют из себя анкеты (questionnaire), которые заполняет владелец животного или клиницист. Наиболее распространёнными являются Health Risk Assessment (HRA), Canine Brief Pain Inventory (CBPI), Helsinki Chronic Pain Index (HCPI), Liverpool Osteoarthritis in Dogs (LOAD), Canine Orthopedic Index (COI), Feline Musculoskeletal Pain Index (FMPI) [4][5][6].…”