Correlation of Semen Analysis and Hypoosmotic Swelling Test with Subsequent Pregnancies

Check, Jerome H.; Nowroozi, Kosrow; Wu, Chung Hsin; Bollendorf, A.

doi:10.3109/01485018808987081

Cited by 26 publications

(14 citation statements)

References 2 publications

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“…A decrease in the number of HOS-positive forms in the ejaculates of men with oligozoospermia, asthenozoospermia, and oligoasthenozoospermia compared with men with normozoospermia has been reported [23]. Positive correlation was also observed between HOS and the fertilizing ability of sperm [21,22,[24][25][26], however, others have not found a significant correlation between HOS and in vitro fertilization (IVF) outcome [27][28][29][30]. Thus, the clinical utility of the HOS test in assessment of sperm function is still debatable.…”

Section: Introductionmentioning

confidence: 80%

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Progesterone Receptor as an Indicator of Sperm Function

Gadkar¹,

Shah²,

Sachdeva³

et al. 2002

Biology of Reproduction

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Expression of progesterone receptor (PR) localization on spermatozoa was determined in men with normal and abnormal spermiograms. Studies were also carried out to evaluate the potential of PR as a marker of sperm function. Progesterone receptor expression on spermatozoa from men with normozoospermia (n = 8), oligozoospermia (n = 7), asthenozoospermia (n = 8), oligoasthenozoospermia (n = 7), and teratozoospermia (n = 11) was analyzed using an immunocytochemical method with monoclonal antibodies against PR, and flow cytometry using a cell-impermeable fluorescein-tagged progesterone coupled to BSA complex (P-FITC-BSA). Both methods revealed significantly fewer (P < 0.05) PR-positive spermatozoa in men with oligozoospermia, asthenozoospermia, oligoasthenozoospermia, and teratozoospermia compared with men with normozoospermia, thereby suggesting that down-regulation of PR expression in spermatozoa may be one of the causes of male infertility. Spermatozoa from men with normozoospermia (n = 12), oligozoospermia (n = 12), asthenozoospermia (n = 12), oligoasthenozoospermia (n = 9), and teratozoospermia (n = 10) were exposed to low osmotic conditions in the hypoosmotic swelling (HOS) test and then analyzed for PR expression using P-FITC-BSA complex. A significantly higher percentage (P < 0.05) of spermatozoa with physiologically active plasma membrane (HOS+) lacked PR expression (HOS+PR-) in all categories of men with infertility, thereby suggesting that compared to the HOS test, PR expression is a better indicator of sperm function. Furthermore, PR expression in spermatozoa showed a strong (P < 0.05) positive correlation with their ability to undergo an in vitro acrosome reaction. This was observed in all study groups (i.e., normozoospermia, r = 0.8545; oligozoospermia, r = 0.8711; asthenozoospermia, r = 0.7645; oligoasthenozoospermia, r = 0.9003; and teratozoospermia, r = 0.8676). This suggests a potential role for PR in the events leading to the acrosome reaction in sperm.

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Section: Introductionmentioning

confidence: 80%

“…The HOS test can predict the fertilizing capacity of human spermatozoa [9,21,22]. A positive HOS test is an indicator of the functional integrity of the sperm membrane as judged by a swollen head and curled tail of spermatozoa when exposed to hypo-osmotic conditions [9].…”

Section: Introductionmentioning

confidence: 99%

Progesterone Receptor as an Indicator of Sperm Function

Gadkar¹,

Shah²,

Sachdeva³

et al. 2002

Biology of Reproduction

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“…Langenbucher et al [35] reported that pregnancies occurred only with ejaculates that had 60% or more HOSpositive spermatozoa. Finally, Check et al [ 14,161 concluded that the results of the HOS test were significantly more reliable than the results of the standard spermiogram in being predictive of conception. These investigators found that no couple, regardless of the standard spermiogram, achieved a pregnancy when the HOS test was abnormal.…”

Section: Data From In Vivo Observationsmentioning

confidence: 99%

The Hypoosmotic Swelling Test: An Update

Jeyendran

Ven

Zaneveld

1992

Archives of Andrology

174

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The hypoosmotic swelling (HOS) test is a relatively new assay used to evaluate the functional integrity of the sperm's plasma membrane. In fact, more studies have been published on the applicability of the HOS test than any other new sperm indicator. The assay is based on the fact that fluid transport occurs across an intact cell membrane under hypoosmotic conditions until equilibrium is reached. Due to the influx of fluid, the cell will expand and bulge, especially in the tail, and this change can be readily observed with a phase contrast microscope. Earlier studies have yielded some confusion regarding the interpretation of the data. This review is an attempt to clarify and update the usefulness of the HOS test as a tool to evaluate the sperm function.

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“…The latter interacts in many of the sperm's physiologic actions, e.g., capacitation, the acrosome reaction, and binding to the zona pellucida. Check et al [12] evaluated this test in a group of infertility patients in whom the female counterpart was diagnosed with an infertility factor that was presumed corrected. Only one of 29 men with normal semen parameters (according to WHO standards) had a subnormal HOS score and did not achieve a pregnancy; 27 of the 28 couples who conceived had HOS scores that were normal or gray-zone (Fig.…”

Section: Hypo-osmotic Swelling (Hos) Testmentioning

confidence: 99%

Evaluation and Treatment of a Male Factor Component to Unexplained Infertility

Check¹,

Nowroozi²,

Lee³

et al. 1990

Archives of Andrology

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The standard semen analysis frequently fails to identify subfertile males even when findings are normal and conversely often fails to identify fertile males with subnormal semen analyses. This has created the need to produce other tests of sperm physiology that will better distinguish a fertile from a subfertile specimen. Understanding more about the nature of the sperm defect should lead to the establishment of more specific and effective therapies. Until that time, it would still be reasonable to try some of the available empirical therapies even though they may work merely by a placebo mechanism.

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Correlation of Semen Analysis and Hypoosmotic Swelling Test with Subsequent Pregnancies

Cited by 26 publications

References 2 publications

Progesterone Receptor as an Indicator of Sperm Function

Progesterone Receptor as an Indicator of Sperm Function

The Hypoosmotic Swelling Test: An Update

Evaluation and Treatment of a Male Factor Component to Unexplained Infertility

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