Correlation of Quantitative Digital Image Analysis with the Glomerular Filtration Rate in Chronic Allograft Nephropathy

Díaz-Encarnación, Montserrat; Griffin, Matthew D.; Slezak, Jeffrey M.; Bergstralh, Erik J.; Stegall, Mark D.; Velosa, Jorge A.; Grande, Joseph P.

doi:10.1046/j.1600-6143.2003.00311.x

Cited by 82 publications

(84 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Because, by protocol, we did not treat SAR, these results provide evidence that the vast majority of asymptomatic infiltrates in 6-mo surveillance biopsies may not be deleterious, at least in the intermediate term, and could be adaptive rather than destructive. Sirius red, a specific staining for collagen types I and III, is considered a precise and reliable estimate of renal cortical extracellular matrix accumulation (15,16). Types I and III collagen represent 80 and 15 to 20%, respectively, of the total collagen synthesized by fibroblasts, and especially the early accumulation of collagen type I, along with collagens III and IV, was found to be a more specific marker for chronic rejection (28).…”

Section: Discussionmentioning

confidence: 99%

“…Studies with surveillance biopsies, both in deceased-and living-donor renal grafts, agree that interstitial fibrosis correlates with renal graft survival and with graft function at 2 yr (14,15). Sirius red, a specific staining for collagen types I and III, is considered a precise and reliable estimate of renal cortical extracellular matrix accumulation and represents the best parameter to predict the development of progressive renal failure (15,16).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Untreated Rejection in 6-Month Protocol Biopsies Is Not Associated with Fibrosis in Serial Biopsies or with Loss of Graft Function

Scholten

Rowshani²,

Cremers³

et al. 2006

Journal of the American Society of Nephrology

View full text Add to dashboard Cite

Donor age, calcineurin inhibitor nephrotoxicity, and acute rejection are the most significant predictors of chronic allograft nephropathy. Protocol biopsies, both in deceased-and living-donor renal grafts, have shown that cortical tubulointerstitial fibrosis correlates with graft survival and function. The impact of not treating subclinical acute rejection (SAR) is less clear. In this study, 126 de novo renal transplant recipients were randomly assigned to receive area-under-the-curve-controlled exposure of either a cyclosporine or a tacrolimus-based immunosuppressive regimen that included steroids, mycophenolate mofetil, and basiliximab induction. Protocol biopsies were taken before and 6 and 12 mo after transplantation. The prevalence of SAR was determined retrospectively. Fibrosis was evaluated by quantitative digital analysis of Sirius red staining in serial biopsies. Donor age correlated significantly with tubulointerstitial fibrosis in pretransplantation biopsies and inferior graft function at month 6 (r ‫؍‬ ؊0.26; P ‫؍‬ 0.033). Acute rejection incidence was 11.5%, and no clinical late rejection occurred. The prevalence of SAR at 6 mo was 30.8% but was not associated with differences in serial quantitative Sirius red staining at 6 or 12 mo, proteinuria, or progressive loss of GFR up to 2 yr. No differences were found in donor variables, histocompatibility, rejection history, or exposure of immunosuppressants. Controlled individualized calcineurin inhibitor exposure and subsequent tapering resulted in a low early acute rejection rate and prevented late acute rejection. Because, by design, we did not treat SAR, these results provide evidence that asymptomatic infiltrates in 6-mo surveillance biopsies may not be deleterious in the intermediate term. There is need for reliable biomarkers to prove that not all cell infiltrates are equivalent or that infiltrates may change with time.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Untreated Rejection in 6-Month Protocol Biopsies Is Not Associated with Fibrosis in Serial Biopsies or with Loss of Graft Function

Scholten

Rowshani²,

Cremers³

et al. 2006

Journal of the American Society of Nephrology

View full text Add to dashboard Cite

show abstract

“…Interstitial fibrosis and tubular atrophy are scored semiquantitatively in the two most frequently used classifications of renal allograft pathology, the Banff (3) and CADI systems (28). However, more precise quantification of the volume of interstitial fibrosis can be obtained by histomorphometry (32) and with the use of collagen-specific stains such as Sirius Red and image analysis techniques (33,34).…”

Section: Interstitial Fibrosis and Tubular Atrophy: Prevalence Risk mentioning

confidence: 99%

Protocol Transplant Biopsies

Rush¹

2006

Clinical Journal of the American Society of Nephrology

View full text Add to dashboard Cite

“…Various kinds of solutions ranging from general purpose and inexpensive software to specific and expensive software are available to quantify the specific substance present. [15] uses the general purpose image processing software named 'Metaview' for staining quantification in which suitable threshold values for Red (R), Green (G) and Blue (B) components are selected to choose the stained area. The area representing positively stained region gives the measure of the substance under study.…”

Section: Discussionmentioning

confidence: 99%

“…Attempts to automate the evaluation of antigen expression are reported in many studies [14][15][16][17][18][19][20], but they just identify the area of positive staining but do not discriminate the difference in depth of staining, which is very crucial for the scoring of spots.…”

Section: Introductionmentioning

confidence: 99%

Datamining Approach for Automation of Diagnosis of Breast Cancer in Immunohistochemically Stained Tissue Microarray Images

Prasad¹

2010

TOMINFOJ

View full text Add to dashboard Cite

Abstract:Cancer of the breast is the second most common human neoplasm, accounting for approximately one quarter of all cancers in females after cervical carcinoma. Estrogen receptor (ER), Progesteron receptor and human epidermal growth factor receptor (HER-2/neu) expressions play an important role in diagnosis and prognosis of breast carcinoma. Tissue microarray (TMA) technique is a high throughput technique which provides a standardized set of images which are uniformly stained, facilitating effective automation of the evaluation of the specimen images. TMA technique is widely used to evaluate hormone expression for diagnosis of breast cancer. If one considers the time taken for each of the steps in the tissue microarray process workflow, it can be observed that the maximum amount of time is taken by the analysis step. Hence, automated analysis will significantly reduce the overall time required to complete the study. Many tools are available for automated digital acquisition of images of the spots from the microarray slide. Each of these images needs to be evaluated by a pathologist to assign a score based on the staining intensity to represent the hormone expression, to classify them into negative or positive cases. Our work aims to develop a system for automated evaluation of sets of images generated through tissue microarray technique, representing the ER expression images and HER-2/neu expression images. Our study is based on the Tissue Microarray Database portal of Stanford university at http://tma.stanford.edu/cgibin/cx?n=her1, which has made huge number of images available to researchers. We used 171 images corresponding to ER expression and 214 images corresponding to HER-2/neu expression of breast carcinoma. Out of the 171 images corresponding to ER expression, 104 were negative and 67 were representing positive cases. Out of the 214 images corresponding to HER-2/neu expression, 112 were negative and 102 were representing positive cases. Our method has 92.31% sensitivity and 93.18% specificity for ER expression image classification and 96.67% sensitivity and 88.24% specificity for HER-2/neu expression image classification.

show abstract

Correlation of Quantitative Digital Image Analysis with the Glomerular Filtration Rate in Chronic Allograft Nephropathy

Abstract: Chronic allograft nephropathy (CAN) is characterized

Cited by 82 publications

References 33 publications

Untreated Rejection in 6-Month Protocol Biopsies Is Not Associated with Fibrosis in Serial Biopsies or with Loss of Graft Function

Untreated Rejection in 6-Month Protocol Biopsies Is Not Associated with Fibrosis in Serial Biopsies or with Loss of Graft Function

Protocol Transplant Biopsies

Datamining Approach for Automation of Diagnosis of Breast Cancer in Immunohistochemically Stained Tissue Microarray Images

Contact Info

Product

Resources

About