Correlation of protein partitioning in aqueous polymer two-phase systems

Features of the acid protease partition in aqueous two-phase systems of polyethylene glycol–phosphate: Chymosin and pepsin

“…an homogenate of a natural product or a protein expressed in a microorganim), the first research step is to determine the partitioning features of the target protein in the pure state and the effect of medium variables on its partition coefficient [6]. Diamond and Hsu [9] found that ln K decreases with the PEG concentration difference between the phases for different proteins of 12,000-100,000 molecular mass. However, proteins such as chymotrysinogen-A [10] showed a positive slope for the plot ln K versus [PEG], but this difference in the partition behaviour was not explained by these authors.…”

“…Applying the Flory Higgins theory of polymer thermodynamic, Diamond and Hsu [9] arrayed the following simple linear relation to correlate protein partitioning in aqueous two-phase system:…”

Thermodynamic modeling of the partitioning of biomolecules in aqueous two-phase systems using a modified Flory–Huggins equation

“…Diamond and Hsu [12] correlated the partition coefficient of solutes in aqueous two-phase systems formed by PEG and dextran and PEG and potassium phosphate by the expression:…”

Study of the serum albumin-polyethyleneglycol interaction to predict the protein partitioning in aqueous two-phase systems

“…Several theoretical models have been proposed to explain the thermodynamics of protein partitioning in aqueous two-phase systems. Diamond and Hsu [3] presented a simple linear semilogarithmic relationship for correlating biomolecule partitioning with respect to the polyethyleneglycol concentration difference between the phases. The relationship proved to be adequate for dipeptide and low-molecular-weight protein partitioning.…”