Correlation of Pneumococcal Antibody Concentration and Avidity with Patient Clinical and Immunologic Characteristics

Fried, Ari J.; Altrich, Michelle; Liu, Hongye; Halsey, John F.; Bonilla, Francisco A.

doi:10.1007/s10875-013-9870-9

Cited by 16 publications

(19 citation statements)

References 19 publications

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“…Furthermore, traditional ELISA methods may fail to detect low affinity interactions with fast dissociation rates and often EC 50 calculation is limited to a symmetrical sigmoid curve (using 4-parameter curve fitting) and data from non-symmetrical curves may be less reliable. Other indirect immunoassays for measuring binding strength include use of chaotropic or dissociating agents like diethylamine (Barkoff et al, 2012) or NH 4 SCN (Almanzar et al, 2013; Fried et al, 2013; Prelog et al, 2013). In this modified ELISA immmuoassay, a relative avidity index is calculated after dissociation of low avidity antibodies (Kneitz et al, 2004; Fried et al, 2013; Prelog et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Other indirect immunoassays for measuring binding strength include use of chaotropic or dissociating agents like diethylamine (Barkoff et al, 2012) or NH 4 SCN (Almanzar et al, 2013; Fried et al, 2013; Prelog et al, 2013). In this modified ELISA immmuoassay, a relative avidity index is calculated after dissociation of low avidity antibodies (Kneitz et al, 2004; Fried et al, 2013; Prelog et al, 2013). A notable caveat associated with assays that use dissociating agents is that antibodies that bind to conformational epitopes can be more easily dissociated from their bound complex due to changes in the epitope conformation.…”

Section: Discussionmentioning

confidence: 99%

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Surface plasmon resonance measurements of plasma antibody avidity during primary and secondary responses to anthrax protective antigen

Lynch

Stewart

Kepler

et al. 2014

Journal of Immunological Methods

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Establishment of humoral immunity against pathogens is dependent on events that occur in the germinal center and the subsequent induction of high-affinity neutralizing antibodies. Quantitative assays that allow monitoring of affinity maturation and duration of antibody responses can provide useful information regarding the efficacy of vaccines and adjuvants. Using an anthrax protective antigen (rPA) and alum model antigen/adjuvant system, we describe a methodology for monitoring antigen-specific serum antibody concentration and avidity by surface plasmon resonance during primary and secondary immune responses. Our analyses showed that following a priming dose in mice, rPA-specific antibody concentration and avidity increases over time and reaches a maximal response in about six weeks, but gradually declines in the absence of antigenic boost. Germinal center reactions were observed early with maximal development achieved during the primary response, which coincided with peak antibody avidity responses to primary immunization. Boosting with antigen resulted in a rapid increase in rPA-specific antibody concentration and five-fold increase in avidity, which was not dependent on sustained GC development. The described methodology couples surface plasmon resonance-based plasma avidity measurements with germinal center analysis and provides a novel way to monitor humoral responses that can play a role in facilitating vaccine and adjuvant development.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Surface plasmon resonance measurements of plasma antibody avidity during primary and secondary responses to anthrax protective antigen

Lynch

Stewart

Kepler

et al. 2014

Journal of Immunological Methods

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“…Previously, we demonstrated that memory B cell responses in young children to both the T cell-dependent antigen CTB and the T cell-independent antigen LPS following vaccination were significantly lower than those achieved after natural infection (17). Antibody avidity has been shown to be a correlate of immune memory and protective immunity in both infection and vaccination for other infectious diseases in children (28)(29)(30)(31). In this study, we evaluated the magnitude and duration of antibody avidity in both children and adults who received two doses of Dukoral, a heatand formalin-inactivated oral cholera vaccine.…”

Section: Discussionmentioning

confidence: 99%

Antibody Avidity in Humoral Immune Responses in Bangladeshi Children and Adults following Administration of an Oral Killed Cholera Vaccine

Alam

Leung

Akhtar

et al. 2013

Clin. Vaccine Immunol.

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e Antibody avidity for antigens following disease or vaccination increases with affinity maturation and somatic hypermutation. In this study, we followed children and adults in Bangladesh for 1 year following oral cholera vaccination and measured the avidity of antibodies to the T cell-dependent antigen cholera toxin B subunit (CTB) and the T cell-independent antigen lipopolysaccharide (LPS) in comparison with responses in other immunological measurements. Children produced CTB-specific IgG and IgA antibodies of high avidity following vaccination, which persisted for several months; the magnitudes of responses were comparable to those seen in adult vaccinees. The avidity of LPS-specific IgG and IgA antibodies in vaccinees increased significantly shortly after the second dose of vaccine but waned rapidly to baseline levels thereafter. CTB-specific memory B cells were present for only a short time following vaccination, and we did not find significant memory B cell responses to LPS in any age group. For older children, there was a significant correlation between CTB-specific memory T cell responses after the second dose of vaccine and CTB-specific IgG antibody avidity indices over the subsequent year. These findings suggest that vaccination induces a longer-lasting increase in the avidity of antibodies to a T cell-dependent antigen than is measured by a memory B cell response to that antigen and that early memory T cell responses correlate well with the subsequent development of higher-avidity antibodies.

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“…For example, some individuals with “protective” levels of pathogen-specific IgG antibody may nevertheless not be protected by virtue of producing an antibody with low avidity or low opsonophagocytic activity. 181,182 However, there are currently no accepted clinical criteria regarding normal vaccine response based on measurements other than specific serum IgG antibody concentration.…”

Section: Diagnosismentioning

confidence: 99%

International Consensus Document (ICON): Common Variable Immunodeficiency Disorders

Bonilla

Barlan

Chapel

et al. 2016

The Journal of Allergy and Clinical Immunology: In Practice

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686

772

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The International Collaboration in Asthma, Allergy and Immunology initiated an international coalition among the American Academy of Allergy, Asthma & Immunology; the European Academy of Allergy and Clinical Immunology; the World Allergy Organization; and the American College of Allergy, Asthma & Immunology on common variable immunodeficiency. An author group was formed and then divided into individual committees. Within the committee, teams of authors were subgrouped to generate content for specific sections of the document. Content was derived from literature searches, relevant published guidelines, and clinical experience. After a draft of the document was assembled, it was collectively reviewed and revised by the authors. Where evidence was lacking or conflicting, the information presented represents the consensus expert opinion of the group. The full document was then independently reviewed by 5 international experts in the field, none of whom was among the authors of the original. The comments of these reviewers were incorporated before submission for publication.

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Correlation of Pneumococcal Antibody Concentration and Avidity with Patient Clinical and Immunologic Characteristics

Abstract: Antibody concentration and avidity correlate with patient clinical characteristics and distinguish patients from controls. Measurement of antibody avidity may provide another dimension for the clinical assessment of pneumococcal polysaccharide antibody response.

Cited by 16 publications

References 19 publications

Surface plasmon resonance measurements of plasma antibody avidity during primary and secondary responses to anthrax protective antigen

Surface plasmon resonance measurements of plasma antibody avidity during primary and secondary responses to anthrax protective antigen

Antibody Avidity in Humoral Immune Responses in Bangladeshi Children and Adults following Administration of an Oral Killed Cholera Vaccine

International Consensus Document (ICON): Common Variable Immunodeficiency Disorders

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