Correlation of Photosynthetic Products of Symbiotic Chlorella with the Mating Reactivity Rhythms in a Mutant Strain of Paramecium bursaria

Tanaka, Miho; Miwa, Ichitomo

doi:10.2108/zsj.17.735

Cited by 7 publications

(2 citation statements)

References 17 publications

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“…Interestingly, synchronous gene expression patterns were found for these symbiotic partners over the course of a day–night cycle, and metabolic modes were switched between day and night in the organ. Additionally, the notable influence of endosymbionts on host circadian rhythms has been reported in the unicellular ciliate Paramecium bursaria 10 , 11 . Its symbiotic algae, Chlorella , enable the host’s mating reactivity rhythm to persist under constant light conditions, as shown by the fact that Chlorella -free cells derived from the same strain fail to display such a rhythm.…”

Section: Introductionmentioning

confidence: 99%

Nighttime activities and peripheral clock oscillations depend on Wolbachia endosymbionts in flies

Morioka

Oida

Tsuchida

et al. 2018

Sci Rep

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Wolbachia are ubiquitous bacterial endosymbionts of arthropods and affect host gene expression. Although Wolbachia infections were suggested to modulate sleep in flies, their influence on the circadian clock remained obscure. Here, we screened bacterial symbionts in a laboratory Drosophila melanogaster colony, and observed widespread infections of wMel strain Wolbachia. We established a Wolbachia-free strain from a clock gene reporter strain, period-luciferase (per-luc). Temperature (19–29 °C)-compensated free-running periods were detected regardless of infections which may reflect the lack of wMel infections in central circadian pacemaker neurons. However, locomotor activity levels during the night or subjective night were significantly amplified in uninfected flies. Moreover, the behavioral phenotype of F1 offspring of an uninfected female and infected male resembled that of uninfected flies. This trait is consistent with maternal transmission of Wolbachia infection. Interestingly, per-luc activities in headless bodies, as an index of peripheral circadian oscillators, were severely damped in uninfected flies. Additionally, circadian amplitudes of PER immunoreactivities in Malpighian tubules were reduced in uninfected flies. These results demonstrate that Wolbachia boost fly peripheral clock oscillations and diurnal behavioral patterns. Genetic mechanisms underlying behavioral rhythms have been widely analyzed using mutant flies whereas screening of Wolbachia will be necessary for future studies.

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Section: Introductionmentioning

confidence: 99%

Nighttime activities and peripheral clock oscillations depend on Wolbachia endosymbionts in flies

Morioka

Oida

Tsuchida

et al. 2018

Sci Rep

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“…Symbiotic Chlorella of P. bursaria releases photosynthetic products, mainly maltose and oxygen, contributing to host energy (see Introduction). Therefore, we examined the effects as shown of the addition of 10 mM maltose on DD‐incubated algae in accordance with previous reports (Tanaka & Miwa, , ). As shown in the right bar of Fig.…”

Section: Resultsmentioning

confidence: 87%

SymbioticChlorella variabilisincubated under constant dark conditions for 24 hours loses the ability to avoid digestion by host lysosomal enzymes in digestive vacuoles of host ciliateParamecium bursaria

Kodama

Fujishima

2014

FEMS Microbiology Ecology

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Endosymbiosis between symbiotic Chlorella and alga-free Paramecium bursaria cells can be induced by mixing them. To establish the endosymbiosis, algae must acquire temporary resistance to the host lysosomal enzymes in the digestive vacuoles (DVs). When symbiotic algae isolated from the alga-bearing paramecia are kept under a constant dark conditions for 24 h before mixing with the alga-free paramecia, almost all algae are digested in the host DVs. To examine the cause of algal acquisition to the host lysosomal enzymes, the isolated algae were kept under a constant light conditions with or without a photosynthesis inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea for 24 h, and were mixed with alga-free paramecia. Unexpectedly, most of the algae were not digested in the DVs irrespective of the presence of the inhibitor. Addition of 1 mM maltose, a main photosynthetic product of the symbiotic algae or of a supernatant of the isolated algae kept for 24 h under a constant light conditions, did not rescue the algal digestion in the DVs. These observations reveal that unknown factors induced by light are a prerequisite for algal resistance to the host lysosomal enzymes.

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Infection of Paramecium bursaria by Symbiotic Chlorella Species

Kodama

Fujishima

2009

Endosymbionts in Paramecium

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Correlation of Photosynthetic Products of Symbiotic Chlorella with the Mating Reactivity Rhythms in a Mutant Strain of Paramecium bursaria

Cited by 7 publications

References 17 publications

Nighttime activities and peripheral clock oscillations depend on Wolbachia endosymbionts in flies

Nighttime activities and peripheral clock oscillations depend on Wolbachia endosymbionts in flies

SymbioticChlorella variabilisincubated under constant dark conditions for 24 hours loses the ability to avoid digestion by host lysosomal enzymes in digestive vacuoles of host ciliateParamecium bursaria

Infection of Paramecium bursaria by Symbiotic Chlorella Species

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