Correlation of photobleaching, oxidation and metal induced fluorescence quenching of DNA-templated silver nanoclusters

Morishita, Kiyoshi; MacLean, James L.; Liu, Biwu; Jiang, Hui; Liu, Juewen

doi:10.1039/c3nr33421j

Cited by 65 publications

(61 citation statements)

References 55 publications

(45 reference statements)

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“…The same trend of sensitivity was observed with light exposure, which is related to oxidation of the AgNCs [73]. It has been concluded that metal ions (usually Hg 2+ , Cu 2+ , Ag + and Au 3+ ) can quench metal NCs due to their high redox potential.…”

Section: Reactive Quencherssupporting

confidence: 58%

“…We found that DNA templated AgNCs were bleached very quickly when exposed to strong light. A wavelength-dependent study indicates that photobleaching takes place only by the UV light, while little effect was observed at wavelengths longer than 400 nm [73]. We attributed photobleaching to oxidation since bleached AgNCs can be recovered after adding NaBH4.…”

Section: Characterization Of Ncsmentioning

confidence: 90%

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DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Liu

2014

TrAC Trends in Analytical Chemistry

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195

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In the past decade, fluorescent silver, gold and copper nanoclusters (Ag, Au and CuNCs) have emerged as a new class of signaling moiety for biosensor development. Compared to semiconductor quantum dots, metal NCs have less toxicity concerns and can be more easily conjugated to biopolymers. Due to their extremely small size, these NCs need a stabilizing ligand. Many polymers, proteins and nucleic acids have been reported to stabilize NCs. In particular, many DNA sequences produce highly fluorescence NCs. Coupling these DNA stabilizers with other sequences such as aptamers has generated a large number of biosensors. In this review, the synthesis of DNA and nucleotide-templated NCs is first summarized; their chemical interactions are also discussed. In the second part, the properties of NCs such as fluorescence quantum yield, emission wavelength and lifetime, structure and photostability are briefly reviewed. In the last part, various sensor design strategies using these NCs are categorized into the following four classes: 1) fluorescence de-quenching; 2) generation of templating DNA sequences to produce NCs; 3) change of nearby environment; and 4) reacting with heavy metal ions or other quenchers. Finally, the future trends in this field are discussed.

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Section: Reactive Quencherssupporting

confidence: 58%

Section: Characterization Of Ncsmentioning

confidence: 90%

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Liu

2014

TrAC Trends in Analytical Chemistry

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195

141

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“…[ 6 ] Not all AgNCs have such excellent chemical and photostability, especially in solution. [ 20,38 ] The fl uorescence decay times are calculated every 10 000 photons and plotted alongside the fl uorescence trajectory (Figure 1 a). Of the 289 C24-AgNCs measured, the overwhelming majority could be fi tted with a monoexponential decay function (285 of 289, 99%).…”

Section: Single Molecule Measurements: Decay Timesmentioning

confidence: 99%

Single‐Molecule Characterization of Near‐Infrared‐Emitting Silver Nanoclusters

Hooley

Paolucci

Liao

et al. 2015

Advanced Optical Materials

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In this paper, single molecule spectroscopy is used to probe the photophysical heterogeneity of near‐infrared emitting silver nanoclusters stabilized in single stranded DNA containing 24 cytosines (C24‐AgNCs). Focusing on this subset of emitters allows us to address and correlate their photophysical parameters. The results suggest that for these near‐infrared emitting C24‐AgNCs, the broad range of observed photophysical properties is due to the spectral heterogeneity of two species, one of which has a broad range of emission maxima and decay times. This conclusion is drawn by analyzing the results of a large number of single C24‐AgNCs where, for the first time, emission maxima, fluorescence decay times, fluorescence intensity, blinking, and photon antibunching are determined simultaneously. The single molecule measurements also reveal that some of the C24‐AgNCs can change their spectral properties during the measurement, while photon antibunching measurements verified that all the analyzed C24‐AgNCs behave as single emitters. While the overall spectral heterogeneity can be related to the immobilization in the polymer film and/or intrinsic heterogeneity of the C24‐AgNCs emitters, the exact origin of the dynamical changes has not been fully determined. However, changes in the AgNCs geometry, local environment, or changes in the DNA conformation are possible explanations.

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“…Many research groups including our own showed that photobleaching of AgNCs is related to oxidation. [33][34][35] In this regard, AuNC might be a useful alternative since gold is more resistant to oxidation than silver. While adsorption of DNA by gold nanoparticles (AuNPs) have been extensively studied since the 1990s, [36][37][38][39][40] using DNA for making AuNCs has been reported only very recently.…”

Section: Introductionmentioning

confidence: 99%

DNA-templated fluorescent gold nanoclusters reduced by Good’s buffer: from blue-emitting seeds to red and near infrared emitters

Lopez

Liu

2015

Can. J. Chem.

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DNA templated fluorescent gold nanoclusters (AuNCs) have been recently prepared, showing higher photostability than the silver counterpart. In this work, we examined the effect of pH, DNA length, sequence and reducing agent. Citrate, HEPES and MES produce blue emitters, glucose and NaBH4 cannot produce fluorescent AuNCs, while ascorbate shows blue emission even in the absence of DNA. This is the first report of using Good's buffer for making fluorescent AuNCs.Dimethylamine borane (DMAB) produces red emitter. Poly-C DNA produces AuNCs only at low pH and each DNA chain can only bind to a few gold atoms, regardless of the DNA length.Otherwise, large nonfluorescent gold nanoparticles (AuNPs) are formed. Each poly-A DNA might template a few independent AuNCs. The blue emitters can be further reduced to form red emitters 2 by adding DMAB. The emission color is mainly determined by the type of reducing agent instead of DNA sequence.

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Correlation of photobleaching, oxidation and metal induced fluorescence quenching of DNA-templated silver nanoclusters

Abstract: Such metal induced quenching cannot be recovered by NaBH4. Electronic absorption and mass spectrometry studies offered further insights into the oxidation reaction. Our results correlate many important experimental observations and will fuel the further growth of this field.2

Cited by 65 publications

References 55 publications

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Single‐Molecule Characterization of Near‐Infrared‐Emitting Silver Nanoclusters

DNA-templated fluorescent gold nanoclusters reduced by Good’s buffer: from blue-emitting seeds to red and near infrared emitters

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