Correlation of oocyte number with serum anti-Müllerian hormone levels measured by either Access or Elecsys in fresh in vitro fertilization cycles

Abstract

Objective: The aim of this study was to assess the correlation of oocyte number with serum anti-Müllerian hormone (AMH) levels measured by two automated methods (Access or Elecsys) in fresh stimulated in vitro fertilization (IVF) cycles.Methods: In this retrospective study at a university hospital, data were collected from 243 fresh stimulated IVF cycles performed from August 2016 to December 2020. The serum AMH level was measured by Access in 120 cycles and by Elecsys in 123 cycles. The cut-off of serum AMH for prediction of poor responders (three or fewer oocytes) or high responders (15 or more oocytes) was calculated by the receiver operating characteristic curve analysis.Results: For the two automated methods, the following equations were derived: total oocyte number=2.378+1.418×(Access-AMH) (r=0.645, p<0.001) and total oocyte number=2.417+2.163×(Elecsys-AMH) (r=0.686, p<0.001). The following combined equation could be derived: (Access-AMH)=0.028+1.525×(Elecsys-AMH). To predict poor responders, the cut-off of Access-AMH was 1.215 ng/mL (area under the curve [AUC], 0.807; 95% confidence interval [CI], 0.730–0.884; p<0.001), and the cut-off of Elecsys-AMH was 1.095 ng/mL (AUC, 0.848; 95% CI, 0.773–0.923; p<0.001). To predict high responders, the cut-off of Access-AMH was 3.450 ng/mL (AUC, 0.922; 95% CI, 0.862–0.981; p<0.001), and the cut-off of Elecsys-AMH was 2.500 ng/mL (AUC, 0.884; 95% CI, 0.778–0.991; p<0.001).Conclusion: Both automated methods for serum AMH measurement showed a good correlation with oocyte number and good performance for predicting poor and high responders in fresh stimulated IVF cycles. The Access method usually yielded higher measured serum AMH levels than the Elecsys method.

Investigation of the Prevalence of Diminished Ovarian Reserve in Korean Women of Reproductive Age

“…Previous studies on AMH in Korea have involved a limited number of subjects, used different cutoffs for diminished ovarian reserve, or focused on continuous variables to define reference intervals [11][12][13][14][15]. Additionally, previous studies on serum AMH, FSH, and IVF have been conducted using data from women who visited university hospitals in Korea [11,14].…”

“…Previous studies on AMH in Korea have involved a limited number of subjects, used different cutoffs for diminished ovarian reserve, or focused on continuous variables to define reference intervals [11][12][13][14][15]. Additionally, previous studies on serum AMH, FSH, and IVF have been conducted using data from women who visited university hospitals in Korea [11,14]. The prevalence of diminished ovarian reserve based on serum AMH and FSH tests in a larger Korean population has not been extensively studied, and there is a lack of recent information about its prevalence as a possible indication for IVF and/or national infertility support programs, particularly in the context of maternal aging.…”