Correlation of LAMA3 with onset and prognosis of ovarian cancer

Abstract

Correlation of laminin subunit α3 ( LAMA3 ) gene with onset and prognosis of ovarian cancer was investigated. In total, 210 ovarian cancer patients who received surgical resection in West China Second Hospital from March 2011 to March 2013 were randomly selected, and another 160 non-ovarian cancer patients who needed ovariectomy were also selected. The relative expression of LAMA3 gene was compared via quantitative polymerase chain reaction (qPCR) in carcinoma tissues, para-carcinoma tissues and non-carcinoma normal tissues in ovarian cancer patients. The methylation level was compared among the above three tissue types. The correlation between the mutation site rs12373237 in LAMA3 gene and onset was analyzed. The expression of laminin in ovarian cancer was detected using immunohistochemistry. Moreover, the 5-year survival rate after operation was recorded and the survival curve was plotted. The expression level of LAMA3 was lower in carcinoma tissues than those in normal tissues and para-carcinoma tissues (P<0.05). The methylation degree was lower in para-carcinoma tissues and normal tissues than that in carcinoma tissues (P<0.05). The CC homozygous mutation of rs12373237 was highly correlated with the onset of ovarian cancer (OR=4.333, P=0.028). The expression of LAMA3 was classified via immunohistochemistry, and the number in high-expression group (63.8%) was larger than that in low-expression group (36.2%) (P<0.05). According to the analysis of 5-year survival rate, the recurrence-free survival rate and overall survival rate in LAMA3 high-expression group were significantly higher than those in LAMA3 low-expression group (P<0.05). The expression level and base mutation of LAMA3 gene can change the level of laminin, which have a certain influence on the onset and prognosis of ovarian cancer.

Diarylpentanoid (1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one) (MS13) Exhibits Anti-proliferative, Apoptosis Induction and Anti-migration Properties on Androgen-independent Human Prostate Cancer by Targeting Cell Cycle–Apoptosis and PI3K Signalling Pathways

“…LAMA3 encodes laminins that are essential for the formation of the basement membrane and plays an additional role in regulating cell migration and mechanical signal transduction (Castro et al, 2018). Increased level of LAMA3 was reported in pancreatic ductal adenocarcinoma, ovarian and liver cancer (Svoboda et al, 2017;Zhang et al, 2019;Tang et al, 2019;Yang et al, 2019). LAMA3 also provides instruction for making a subunit of a protein called laminin 332, promoting cell migration in cancer cells (Carpenter et al, 2017;Oh et al, 2017).…”

Identification of DNA methylation-driven genes and construction of a nomogram to predict overall survival in pancreatic cancer

“…LAMA3 (laminin subunit α3) encodes laminin, which is involved in regulating cell migration [37]. LAMA3 also regulates the expression of different types of cell growth factors that mediate cell proliferation, including KGF (keratinocyte growth factor), EGF (epidermal growth factor), and IGF (insulin-like growth factor) [38]. The LAMA3expression level in tumor tissues and its effect on survival varies between cancer types.…”

“…The LAMA3expression level in tumor tissues and its effect on survival varies between cancer types. Lin et al found that the expression level of LAMA3 in tumor tissues was lower than in normal tissues, and the survival times of ovarian cancer patients with LAMA3 overexpression was better than those with low-expression [38]. In PC, the expression level of LAMA3 in carcinoma tissue is upregulated, and patients with high LAMA3 expression have poor outcomes [39,40].…”

LAMA3 DNA methylation and transcriptome changes associated with chemotherapy resistance in ovarian cancer

“…LAMA3 is important in development by interacting with other extracellular matrix components. LAMA3 and its variants expressions are exacerbated that increase the motility and invasiveness in ovarian cancer [ 27 , 28 ]. Tang found that LAMA3 is abnormally hypermethylated in ovarian cancer, but the relationship between LAMA3 methylation and chemotherapy resistance in ovarian cancer has not been reported.…”