Correlation of in vivo and in vitro functions of fresh and stored human platelets

Rothwell, Stephen W.; Maglasang, P.; Reid, Thomas J.; Gorogias, Martha; Krishnamurti, Chitra

doi:10.1046/j.1537-2995.2000.40080988.x

Cited by 26 publications

(24 citation statements)

References 20 publications

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“…Rothwell et al (20) showed that platelets mounting 22% of the baseline aggregation function (after storage) were still able to provide adequate hemostatic function in an in vivo kidney bleeding model. No clinical studies have correlated in vitro results of aggregation tests with clinical effectiveness of platelet transfusions in actively bleeding patients or have studied the effect of transfused platelets with different aggregation responses on posttransfusion bleeding times.…”

Section: Discussionmentioning

confidence: 99%

The Functional Integrity of Platelets in Volume-Reduced Platelet Concentrates

Schoenfeld¹,

Muhm²,

Doepfmer³

et al. 2005

Anesthesia & Analgesia

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Premature and low-birth-weight infants usually require small-volume platelet transfusions to treat thrombocytopenia. Also, infants undergoing open-heart surgery with extracorporeal circulation and with compromised cardiac function are at risk for excessive intravascular volume. The small-volume platelet substitution can be achieved by dispensing an aliquot from the unit of a standard single-donor platelet concentrate (PC). Alternatively, there is an indication for volume reduction of PCs to maximize the number of platelets transfused in the smallest possible volume. We determined the spontaneous and induced activation of platelets before and after volume reduction in 20 consecutive single-donor-apheresis PCs. After a mean storage time of 2 days, the PCs were plasma-depleted by centrifugation. Spontaneous, adenosine diphosphate (ADP)-induced, and collagen-induced activation were determined by flow cytometry. Furthermore, ADP- and collagen-induced aggregation were measured. A total of 33.8% of platelets in standard PCs were activated spontaneously. Volume reduction of PCs led to a mild but significant increase of spontaneous activation of platelets (43.2%). Additionally, volume reduction resulted in an impaired ADP-induced aggregability of platelets, whereas collagen induction was unaffected. Transfusion of volume-reduced PCs is an effective alternative to use of standard PCs in patients at frequent risk for excessive intravascular volume, because equal volumes increase the platelet count twice as effectively.

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Section: Discussionmentioning

confidence: 99%

The Functional Integrity of Platelets in Volume-Reduced Platelet Concentrates

Schoenfeld¹,

Muhm²,

Doepfmer³

et al. 2005

Anesthesia & Analgesia

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“…Reports of refrigerated storage on coagulation properties of WB are virtually limited to coagulation proteins but not PLTs, 11 and there are no evaluations of integrated coagulation function. Storage effects have been studied extensively on refrigerated separated blood components of plasma and PLTs, suggesting that the integrity of plasma protein factors and PLT function may not fall below clinically useful levels during refrigerated storage until well beyond our current 48‐hour limit 11‐14 . Stimulated by the reports from the military and our observations in pediatric cardiac surgery, we wanted to learn the limits of storage of WB with regard to preservation of its beneficial hemostatic properties.…”

mentioning

confidence: 99%

Toward a definition of “fresh” whole blood: an in vitro characterization of coagulation properties in refrigerated whole blood for transfusion

et al. 2011

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Background The hemostatic property of “fresh” whole blood (WB) has been observed in military application and cardiac surgery and is associated with reduced blood loss, transfusion requirements, and donor exposures. The time from donation to transfusion defining “fresh” has not been systematically studied. We undertook an in vitro study of coagulation properties of refrigerated WB stored for 31 days. Study design and methods Twenty-one WB units were obtained from healthy volunteer donors and stored under standard AABB refrigerated conditions. Samples were obtained on the day after donation and again on Days 2, 4, 7, 11, 14, 17, 21, 24, and 31. Tests included complete blood count, pH, pO2, pCO2, glucose, lactate, thromboelastography (TEG), and platelet function by light transmission aggregometry (LTA). Results There was progressive decline in pH, pO2, glucose, and sodium, but progressive increase in potassium, pCO2, and lactate. TEG variables in all units were normal through Day 11; abnormal values in some variables in some units began on Day 14. Final aggregation levels exhibited no change from Day 1 to Day 21 with adenosine diphosphate and epinephrine, but a decline with collagen (Day 7) and ristocetin (Day 17). Conclusion This in vitro study of coagulation properties demonstrates preservation of normal integrated coagulation function to a minimum of 11 days under standard conditions of refrigerated storage of WB for transfusion. These observations strongly suggest that the hemostatic quality of WB may extend beyond current transfusion practices. If confirmed clinically, this would increase availability and extend benefits of reduced donor exposure and transfusion requirements.

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“…Platelet sediments were washed twice in Tyrode buffer (pH 7.4) and then suspended in it (1 mL). Afterwards, platelets were frozen and stored according to previously published recommendations [21], [22].…”

Section: Methodsmentioning

confidence: 99%

Association between the Perioperative Antioxidative Ability of Platelets and Early Post-Transplant Function of Kidney Allografts: A Pilot Study

2012

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BackgroundRecent studies have demonstrated that the actions of platelets may unfavorably influence post-transplant function of organ allografts. In this study, the association between post-transplant graft function and the perioperative activity of platelet antioxidants was examined among kidney recipients divided into early (EGF), slow (SGF), and delayed graft function (DGF) groups.Methodology/Principal FindingsActivities of superoxide dismutase, catalase, glutathione transferase (GST), glutathione peroxidase, and glucose-6-phosphate dehydrogenase (G6P) were determined and levels of glutathione, oxidized glutathione, and isoprostane were measured in blood samples collected immediately before and during the first and fifth minutes of renal allograft reperfusion. Our results demonstrated a significant increase in isoprostane levels in all groups. Interestingly, in DGF patients, significantly lower levels of perioperative activity of catalase (p<0.02) and GST (p<0.02) were observed. Moreover, in our study, the activity of platelet antioxidants was associated with intensity of perioperative oxidative stress. For discriminating SGF/DGF from EGF, sensitivity, specificity, and positive and negative predictive values of platelet antioxidants were 81–91%, 50–58%, 32–37%, and 90–90.5%, respectively.ConclusionsDuring renal transplantation, significant changes occur in the activity of platelet antioxidants. These changes seem to be associated with post-transplant graft function and can be potentially used to differentiate between EGF and SGF/DGF. To the best of our knowledge, this is the first study to reveal the potential protective role of platelets in the human transplantation setting.

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Correlation of in vivo and in vitro functions of fresh and stored human platelets

Cited by 26 publications

References 20 publications

The Functional Integrity of Platelets in Volume-Reduced Platelet Concentrates

The Functional Integrity of Platelets in Volume-Reduced Platelet Concentrates

Toward a definition of “fresh” whole blood: an in vitro characterization of coagulation properties in refrigerated whole blood for transfusion

Association between the Perioperative Antioxidative Ability of Platelets and Early Post-Transplant Function of Kidney Allografts: A Pilot Study

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