Correlation of IL-1 and HB-EGF with endometrial receptivity

Abstract

Fluctuations of interleukin-1β (IL-1β) and heparin-binding epidermal growth factor (HB-EGF) in endometrial receptivity were detected. Seventy-two patients receiving in vitro fertilization-embryo transfer (IVF-ET) for the first time in Yantaishan Hospital from July 2015 to September 2015 due to infertility were selected. The serum and follicular fluid of patients in ovulation-promoting cycle were collected; the levels of IL-1β and HB-EGF in serum and follicular fluid were detected via enzyme-linked immunosorbent assay (ELISA), and the levels of serum estradiol (E2), progesterone (P), follicle stimulating hormone (FSH) and luteinizing hormone (LH) were detected. The endometria in early follicular phase and middle luteal phase were collected, and the mRNA expression levels of IL-1β and HB-EGF were evaluated by reverse transcription quantitative polymerase chain reaction (RT-qPCR). Patients were divided into two groups, and the implantation group (n=33) and the non-implantation group (n=39), according to whether embryos were implanted and the general data. In IVF-ET, the levels of IL-1β and HB-EGF in follicular fluid and middle luteal phase, the level of serum IL-1β on human chorionic gonadotropin (HCG) day and embryo transfer (ET) day, the levels of E2, FSH and LH on HCG day in implantation group were obviously higher than those in non-implantation group (p<0.05); the level of P on ET day in implantation group was significantly higher than that in non-implantation group (p<0.05); the expression levels of IL-1β and HB-EGF in endometrium in middle luteal phase in implantation group were higher than those in non-implantation group (p<0.05); the expression levels of IL-1β and HB-EGF in endometrium were positively correlated with the levels of E2 and P, and endometrial thickness (p<0.05). IL-1β and HB-EGF may improve the endometrial receptivity to embryo, thus affecting the embryo implantation rate, through the synergistic action with E2 and P, so they may be the indexes of predicting the IVF-ET pregnancy outcome.

The Biological Function of Extracellular Vesicles during Fertilization, Early Embryo—Maternal Crosstalk and Their Involvement in Reproduction: Review and Overview

“…The HB-EGF is one of the members of epidermal growth factor family and, through the HB-EGF receptors on the surface of embryo and endometrium, facilitates the process of implantation and promotes the development of blastocyst [90,91]. The expression levels of IL-1β and HB-EGF are higher in implantation phase and are positively correlated with the levels of estradiol and progesterone [92], showing their important role in this phase. Integrins are adhesion molecules and constitute a system of cell-cell and cell-matrix interaction.…”

Design and evaluation of a novel nanodrug delivery system for reducing the side effects of clomiphene citrate on endometrium

“…All data have been presented as the mean ± SEM. The statistical significance of differences between the groups was determined using one-way analysis of variance (ANOVA), followed by Tukey's post hoc test increased estradiol levels in women undergoing IVF, HBEGF levels [32] and cytokines involved in implantation such as CSF [33] decreased and also the endometrial thickness decreased, whereas the implantation was failed. In this study, the mRNA levels of LIF, LIFR, HOXA10, HBEGF, and EGF were significantly upregulated and MUC1 and PGR mRNA levels were significantly downregulated in the CC-containing PBF-treated animals compared with only CC group (P < 0.05).…”

Endometrial Receptivity on Assisted Reproductive Technology Cycles – a correlation between Serial Serum Estradiol levels and Serial Endometrial Volume Assessment

“…The majority showed no impact. (1)(2)(3)(4)(5) A metanalysis was conducted in 2019 and no quality evidence was found to support or refute the value of estradiol levels on the day of hCG administration, as a predictor of pregnancy in ART cycles. (6) Con icting results may relate to the difference between the way the trials were conducted, the difference of stimulation protocol, number of embryos transferred, and the de nition of outcome in terms of pregnancy rates.…”