Correlation of Hsp110 expression with caspase‐3 and ‐9 during apoptosis induced by <i>in vivo</i> embryonic exposition to retinoic acid or irradiation in early mouse craniofacial development

Gashegu, Julien; Vanmuylder, Nathalie; Philippson, C.; Choa-Duterre, M; Rooze, Marcel; Louryan, Stéphane

doi:10.1111/j.1601-6343.2006.00361.x

Cited by 5 publications

(6 citation statements)

References 44 publications

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“…The Hsp90 family helped cells resist oxidative stress, prevent apoptosis and regulate reproduction via various ways (Han et al, 2007; Hua et al, 2007; Pan et al, 2009; Zhang et al, 2005). Both Hsp105 and Hsp110 not only played roles in embryonic development but also protected embryos through inhibiting apoptosis (Gashegu et al, 2006; Yamagishi et al, 2002). Apg-1 played an important role following transient forebrain ischemia in the rat brain (Xue et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Gene expression of Hsp70, Hsp90 and Hsp110 families in normal palate and cleft palate during mouse embryogenesis

et al. 2012

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Most previous studies focused on a small number of heat shock proteins (Hsps) and their relationships with embryogenesis, and the actual roles of these Hsps in normal and abnormal embryonic development remain unclear. It was found in the present systemic study that except for Grp170, whose expression was not detectable at GD18, all 19 Hsps of Hsp70, Hsp90 and Hsp110 families were expressed in the normal development of embryonic palate tissue in mice, but their expression patterns varied with different Hsps, presenting as a correlation with the developmental phases. In the treatment group by all-trans retinoic acid (atRA), the messenger RNA (mRNA) abundance of HspA1A, HspA1L, HspA8, HspA9, HspA12A, HspA12B, HspA13, HspA14, Hsp90AA1, Hsp90AB1, Grp94, Trap1, Hsp105, Hsp110 and Grp170 was higher in the palates at GD11 (the beginning of palate development), the mRNA abundance of HspA1A, HspA12A and HspA12B was higher at GD18 (before birth) and an mRNA expression peak of HspA1L, HspA8, HspA9, Hsp90AA1, Grp94, Hsp110 and Grp170 was observed at GD17. The mRNA abundance of most genes in atRA-induced cleft palates of the treatment group was different from that of the control group. Grp78, HspA14 and Hsp105 were closely associated with the normal palate development and cleft palate in mouse embryo, possibly as palate development-related genes. Except Grp170, the other genes may be closely associated with the development of mouse palates through participating in the stress response process and/or the antiapoptosis process.

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Section: Discussionmentioning

confidence: 99%

Gene expression of Hsp70, Hsp90 and Hsp110 families in normal palate and cleft palate during mouse embryogenesis

et al. 2012

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“…As regards the physiological apoptosis, the RA‐induced apoptosis is not mediated through caspase‐9 activation, the classical initiator caspase in the mitochondrial apoptosis pathway. However, caspase‐9 activation has been shown in RA‐induced apoptosis within neural crest cells (Gashegu et al. 2006).…”

Section: Discussionmentioning

confidence: 99%

“…However, caspase-9 activation has been shown in RA-induced apoptosis within neural crest cells (Gashegu et al 2006). The caspase activation cascade is probably dependent on the type of cells undergoing cell death, as reported by others (Kuida et al 1996(Kuida et al , 1998Haken et al 1998;Varfolomeev et al 1998;Singh et al 2001).…”

Section: Discussionmentioning

confidence: 99%

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HSP110, caspase‐3 and ‐9 expression in physiological apoptosis and apoptosis induced by in vivo embryonic exposition to all‐trans retinoic acid or irradiation during early mouse eye development

Gashegu¹,

Ladha²,

Vanmuylder³

et al. 2007

Journal of Anatomy

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Apoptosis is an essential physiological process in embryonic development. In the developing eye of vertebrates, three periods of developmental apoptosis can be distinguished: early, intermediate and later. Within the apoptosis pathway, caspases play a crucial role. It has also been shown that HSP110 may have a potential role in apoptosis.The aim of this research was to study the expression of HSP110, caspase-3 and -9 in physiological, retinoic-or irradiation-induced apoptosis during early eye development. Seven pregnant C57Bl/6J mice received 80 mg kg − 1 of all-trans retinoic acid mixed with sesame oil. Seven pregnant NMRI mice received 2 Gy irradiation at the same gestational day. Control mice of both strains (seven mice of each) were not submitted to any treatment. Embryos were harvested at 3, 6, 12 and 24 h after exposition, fixed, dehydrated and embedded. Coronal sections (5 µ m) were made. Slide staining occurred alternatively using anti-caspase-3, anti-caspase-9 and anti-HSP110 immunohistochemistry. HSP110 and caspase-3 expression presented similar topographic and chronological patterns, whereas expression of HSP110 was more precocious in retinoic acid-treated embryos. After retinoic exposure, caspase-3-and HSP110-positive cells were increased in the region of the optic vesicle. By contrast, after irradiation, caspase-3-and HSP110-positive cells were noticeably increased in the optic vesicle, peri-optical mesoderm but less in lens placode. HSP110 was expressed before caspase-3. By contrast, caspase-9 was expressed by a very small number of cells in the optic vesicle either under physiological or under teratogenic conditions. Thus, it seems that activation of caspase-9 is dispensable in early eye developmental apoptosis.

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“…Previous studies showed that Hsp32, Hsp30, Hsp27, Hsp26, Hsp25, Hsp23, and Hsp22 of the sHsps family, 14,23 –27 Hsp40 of the Hsp40 family, 28 Hsp60, Hsp62 and Hsp56 of the Hsp60 family, 29 –31 HspA1A, HspA1B, HspA2, Grp78, HspA8, HspA9, HspA12B and HspA14 of the Hsp70 family, 32 –39 Hsp90AA1, Hsp90AB1, Grp94, and Trap1 of the Hsp90 family, 40 –43 and Hsp105 and Hsp110 of the Hsp110 family 17,44 were expressed in embryonic tissues of different animals.…”

Section: Discussionmentioning

confidence: 99%

Gene expression ofHspsin normal and abnormal embryonic development of mouse hindlimbs

Yan

Wei

Ren³

et al. 2014

Hum Exp Toxicol

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Heat shock proteins (Hsps), which have important biological functions, are a class of highly conserved genetic molecules with the capacity of protecting and promoting cells to repair themselves from damage caused by various stimuli. Our previous studies found that Hsp25, HspB2, HspB3, HspB7, Hsp20, HspB9, HspB10, and Hsp40 may be related to all-trans retinoic acid (atRA)-induced phocomelic and other abnormalities, while HspA12B, HspA14, Trap1, and Hsp105 may be forelimb development-related genes; Grp78 may play an important role in forelimb development. In this study, the embryonic phocomelic, oligodactylic model of both forelimbs and hindlimbs was developed by atRA administered per os to the pregnant mice on gestational day 11, and the expression of 36 members of Hsps family in normal and abnormal development of embryonic hindlimbs was measured by real-time fluorescent quantitative polymerase chain reaction (qRT-PCR). It is found that HspA1L, Hsp22, Hsp10, Hsp60, Hsp47, HspB2, HspB10, HspA12A, Apg1, HspB4, Grp78, and HspB9 probably performs a major function in limb development, and HspA13, Grp94 and Hsp110 may be hindlimb development-related genes.

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Correlation of Hsp110 expression with caspase‐3 and ‐9 during apoptosis induced by in vivo embryonic exposition to retinoic acid or irradiation in early mouse craniofacial development

Cited by 5 publications

References 44 publications

Gene expression of Hsp70, Hsp90 and Hsp110 families in normal palate and cleft palate during mouse embryogenesis

Gene expression of Hsp70, Hsp90 and Hsp110 families in normal palate and cleft palate during mouse embryogenesis

HSP110, caspase‐3 and ‐9 expression in physiological apoptosis and apoptosis induced by in vivo embryonic exposition to all‐trans retinoic acid or irradiation during early mouse eye development

Gene expression ofHspsin normal and abnormal embryonic development of mouse hindlimbs

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