Correlation of host immune response with quantitative recovery of Chlamydia trachomatis from the human endocervix

Brunham, R. C.; Kuo, Cho‐Chou; Cles, Linda; Holmes, King K.

doi:10.1128/iai.39.3.1491-1494.1983

Cited by 180 publications

(71 citation statements)

References 21 publications

(12 reference statements)

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“…In addition to the neutralizing antibodies present in the mucosal surface, the non-speci®c anatomical defences and the components of the innate immune response, are the only barriers in the initial encounter with the pathogen that can protect against infection. 8,16,30 In this respect, we detected the presence of IgA chlamydial speci®c antibodies in the vaginal washes from the three strains of mice. Passively transferred neutralizing monoclonal IgA antibodies able to protect in a murine model against a genital chlamydial challenge have been described.…”

Section: Discussionmentioning

confidence: 97%

Induction of protective immunity against a Chlamydia trachomatis genital infection in three genetically distinct strains of mice

2003

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SUMMARYTo establish the feasibility of inducing a protective immune response against a chlamydial genital infection in animals with different genetic backgrounds, groups of C3H/HeN (H-2 k ), BALB/c (H-2 d ) and C57BL/6 (H-2 b ) mice, were immunized intranasally with elementary bodies (EB) of the Chlamydia trachomatis mouse pneumonitis biovar. Following the intranasal immunization strong Chlamydia-speci®c humoral and cell-mediated immune (CMI) responses were detected in the three strains of mice. Eight weeks following immunization the animals were challenged with C. trachomatis in the genital tract. Vaginal cultures showed that the three strains of mice immunized with EB were signi®cantly protected in comparison to the sham immunized animals. To determine the ability of this immunization protocol to protect against infertility six weeks after the genital challenge the animals were mated. Mice of the three strains immunized with EB showed signi®cant protection as demonstrated by the number of animals that were fertile, and the number of embryos present in their uterine horns, in comparison to the sham immunized mice.

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Section: Discussionmentioning

confidence: 97%

Induction of protective immunity against a Chlamydia trachomatis genital infection in three genetically distinct strains of mice

2003

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“…Chlamydial infectious load in cervical samples was determined as infection-forming units (IFUs)/mL, as described elsewhere [34].…”

Section: Quantification Of Chlamydial Infectious Load In Cervical Sammentioning

confidence: 99%

“…The chlamydial infectious load was determined by inoculating cervical samples into HeLa 229 cells as described elsewhere [34], and IFUs/mL were quantified. In cervical mucosa, the number of mDCs/cervical sample showed a significant correlation with chlamydial IFUs/mL (r = 0.524; p <0.05) in Chlamydia-positive women without MPC.…”

Section: Correlation Of MDC and Pdc With Chlamydial Infectious Load Amentioning

confidence: 99%

Recruitment of myeloid and plasmacytoid dendritic cells in cervical mucosa during Chlamydia trachomatis infection

Agrawal

Vats

Wallace

et al. 2009

Clinical Microbiology and Infection

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The mobilization of myeloid dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs) to the cervix during chlamydial infection is not fully understood, and the role of these cells in immunopathogenesis is largely unknown. As an effective vaccine to control chlamydial infection is currently unavailable, understanding the regulation of the local immune response becomes a necessity. Therefore, mDC and pDC populations were analysed in peripheral blood and cervical samples of controls and Chlamydia-positive women, with or without mucopurulent cervicitis (MPC). Cervical cytokines and C-reactive protein levels in serum were quantified by ELISA and the chlamydial infectious load by culture. Chlamydia trachomatis infection mobilized both mDCs and pDCs to the cervical mucosa. pDCs were recruited more often in women with MPC (p <0.05) and they correlated significantly with the chlamydial load, C-reactive protein levels and cervical interleukin-8 (IL-8) levels. Upregulation of surface expression of co-stimulatory molecules (CD80, CD83 and CD86) on cervical mDCs and pDCs was observed during chlamydial infection but was significant only for mDCs. Significantly higher levels of IL-1 beta, IL-6 and IL-8 were observed in Chlamydia-positive women with MPC; however, after therapy, IL-8 levels decreased significantly. Median numbers of mDCs after therapy were significantly higher in the cervix and blood of infected women as compared to the numbers of pDCs, which were found to be lower in the cervix after therapy. These results thus suggest that during chlamydial infection, both mDCs and pDCs are recruited to the cervix, but their number and possible immunological functions may differ with the pathological condition. pDCs were associated more often with MPC and inflammatory factors, suggesting that they may possibly be involved in the immunopathogenesis of infections due to Chlamydia.

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“…Therefore enhanced activity of the Th2 response, which is characteristic of the cervical mucosal immune system, and the production of secretory immunoglobulin A (sIgA), 17 could be of some benefit for the generation of HPV-neutralizing antibodies within both IgG and IgA classes. Indeed, an inverse correlation between the local concentration of specific sIgA and the susceptibility to various pathogens has been reported, 90,91 suggesting that sIgA may be important for protection against HPV. Th2-derived cytokines, e.g.…”

Section: Regulation Of Anti-hpv Humoral and Cellular Immunity By Cytomentioning

confidence: 99%

Immunology of HPV infection and HPV‐associated tumors

Majewski¹,

Jabłońska²

1998

Int J Dermatology

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Correlation of host immune response with quantitative recovery of Chlamydia trachomatis from the human endocervix

Cited by 180 publications

References 21 publications

Induction of protective immunity against a Chlamydia trachomatis genital infection in three genetically distinct strains of mice

Induction of protective immunity against a Chlamydia trachomatis genital infection in three genetically distinct strains of mice

Recruitment of myeloid and plasmacytoid dendritic cells in cervical mucosa during Chlamydia trachomatis infection

Immunology of HPV infection and HPV‐associated tumors

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