Correlation of Gingival Crevicular Fluid Interleukin‐18 and Monocyte Chemoattractant Protein‐1 Levels in Periodontal Health and Disease

Pradeep, A.R.; Happy, Daisy; Hadge, Parag; Garg, Gaurav; Thorat, Manojkumar

doi:10.1902/jop.2009.090117

Cited by 52 publications

(49 citation statements)

References 39 publications

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“…Pradeep et al [23, 24] gave a good description in their studies on how to perform this collection technique. In this systematic review, 24 studies used this technique (Table 2).…”

Section: Gingival Crevicular Fluid Collection Methodsmentioning

confidence: 99%

Identification of Gingival Crevicular Fluid Sampling, Analytical Methods, and Oral Biomarkers for the Diagnosis and Monitoring of Periodontal Diseases: A Systematic Review

et al. 2016

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Background. Several studies in the last decades have focused on finding a precise method for the diagnosis of periodontal disease in its early stages. Aim. To evaluate from current scientific literature the most common and precise method for gingival crevicular fluid (GCF) sample collection, biomarker analytical methods, and the variability of biomarker quantification, even when using the same analytical technique. Methodology. An electronic search was conducted on in vivo studies that presented clinical data on techniques used for GCF collection and biomarker analysis. Results. The results showed that 71.1%, 24.7%, and 4.1% of the studies used absorption, microcapillary, and washing techniques, respectively, in their gingival crevicular fluid collection. 73.1% of the researchers analyzed their samples by using enzyme-linked immunosorbent assay (ELISA). 22.6%, 19.5%, and 18.5% of the researchers included interleukin-1 beta (IL-1β), matrix metalloproteinase-8 (MMP-8), and tumor necrosis factor-alpha (TNF-α), respectively, in their studies as biomarkers for periodontal disease. Conclusion. IL-1β can be considered among the most common biomarkers that give precise results and can be used as an indicator of periodontal disease progression. Furthermore, paper strips are the most convenient and accurate method for gingival crevicular fluid collection, while enzyme-linked immunosorbent assay can be considered the most conventional method for the diagnosis of biofluids.

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“…Pradeep et al [23, 24] gave a good description in their studies on how to perform this collection technique. In this systematic review, 24 studies used this technique (Table 2).…”

Section: Gingival Crevicular Fluid Collection Methodsmentioning

confidence: 99%

Identification of Gingival Crevicular Fluid Sampling, Analytical Methods, and Oral Biomarkers for the Diagnosis and Monitoring of Periodontal Diseases: A Systematic Review

et al. 2016

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“…In a later publication by Pradeep et al . (12) the outstanding result obtained was that IL-18 was higher in the crevicular fluid of patients with periodontitis and had a positive correlation with the seriousness of the disease.…”

Section: Resultsmentioning

confidence: 98%

“…Three of the case-control studies analyze IL-18 (4,11,12), employing the ELISA technique. Two of these publications (11,12) coincide in finding a lower amount of IL-18 in the control group and a higher amount in the periodontitis group, with intermediate levels for gingivitis.…”

Section: Discussionmentioning

confidence: 99%

Salivary and crevicular fluid interleukins in gingivitis

Boronat-Catalá¹,

Catalá-Pizarro

2014

J Clin Exp Dent

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Introduction: Gingivitis is a frequent inflammatory process of the gum tissue that is mainly caused by the accumulation of plaque. The immune response against inflammatory processes is regulated in part by cytokines. Aims: Given that a continuous inflammation exists in gingivitis, it would be logical to assume that the interleukins will be altered locally in those patients. Therefore, the aim of this review was to check whether there is evidence that the interleukins can be used as diagnostic markers of inflammation levels in patients with gingivitis. Materials and Methods: A bibliographical search was undertaken using the key words interleukin and gingivitis in Pubmed, Cochrane, Scopus and Embase. Only those articles published over the last 10 years that were systematic reviews, case-controls or cohort studies in which interleukins in saliva and/or crevicular fluid was investigated in patients with gingivitis were selected. Results: Finally 15 articles were selected, all of them being case-control studies. The interleukins analyzed in the reviewed articles were: IL-1β, IL-8, IL-18, IL-11, IL-12, TNFα, IL-4, IL-17, IL-1α and IL-6. The most commonly studied interleukin is IL-1β and most authors agree that it is higher in the saliva and/or crevicular fluid of patients with gingivitis. Therefore, it could be used as a diagnostic marker of the degree of inflammation in gingivitis. Moreover, as far as the other interleukins studied are concerned, there is no clear consensus among the authors. Conclusion: There is sufficient evidence to suggest that IL-1β in saliva and/or crevicular fluid can be used as a marker of the degree of inflammation in gingivitis. Key words:Interleukins, gingivitis, saliva, crevicular fluid.

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“…A study by Pradeep et al [18] examined the gingival crevicular fluid (GCF) levels of MCP-1 in periodontal health and disease and indicated that MCP-1 concentrations increased in periodontal disease compared to health and correlated positively with the severity of disease. Tonetti et al [19] reported marked expression of MCP-1 in gingival biopsies and in the inflammatory infiltrate from diseased periodontal sites.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Effects of Nicotine, Cigarette Smoke Condensate, and Porphyromonas gingivalis on Monocyte Chemoattractant Protein-1 Expression from Cultured Human Gingival Fibroblasts

Allam¹,

Recupito²,

Mohamed³

et al. 2015

J Interdiscipl Med Dent Sci

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Background: Monocyte chemoattractant protein-1 (MCP-1) is an inducible protein that attracts monocytes to areas of injury and infection. Studies have shown that it is produced by gingival cells in periodontal diseases and that its incidence increases with the severity of the disease. The aim of the present study was to investigate the effects of nicotine, cigarette smoke condensate (CSC), and Porphyromonas gingivalis (P. gingivalis) on MCP-1 expression from human gingival fibroblasts (HGFs) in vitro.Methods: HGFs were exposed for 72 h to 250 µg/mL of nicotine, 100 µg/mL of CSC, 10% P. gingivalis supernatant, P. gingivalis supernatant with nicotine, or P. gingivalis supernatant with CSC. A control group comprised HGFs without any treatment. The conditioned media was then collected for MCP-1 analysis by enzymelinked immunosorbent assay (ELISA).Results: There were significant differences in MCP-1 level between the P. gingivalis (p=0.0432) and P. gingivalis with CSC (p=0.0037) groups when compared to the control group.Conclusions: P. gingivalis stimulates an inflammatory response in periodontal tissues by increasing MCP-1, which helps attract host cells to combat the bacterial infection. Tobacco usage can mask the inflammatory responses normally seen in periodontal diseases by reducing the levels of MCP-1, thus allowing the bacteria to grow somewhat undetected. This could be one factor that explains why smoking is a major contributing factor to the initiation, development, and progression of periodontal diseases.

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Correlation of Gingival Crevicular Fluid Interleukin‐18 and Monocyte Chemoattractant Protein‐1 Levels in Periodontal Health and Disease

Cited by 52 publications

References 39 publications

Identification of Gingival Crevicular Fluid Sampling, Analytical Methods, and Oral Biomarkers for the Diagnosis and Monitoring of Periodontal Diseases: A Systematic Review

Identification of Gingival Crevicular Fluid Sampling, Analytical Methods, and Oral Biomarkers for the Diagnosis and Monitoring of Periodontal Diseases: A Systematic Review

Salivary and crevicular fluid interleukins in gingivitis

In Vitro Effects of Nicotine, Cigarette Smoke Condensate, and Porphyromonas gingivalis on Monocyte Chemoattractant Protein-1 Expression from Cultured Human Gingival Fibroblasts

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