Correlation of focal adhesion assembly and disassembly with cell migration on nanotopography

Liang, Elena I.; Mah, Emma J.; Yee, Albert F.; Digman, Michelle A.

doi:10.1039/c6ib00193a

Cited by 41 publications

(40 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Results have shown that cellular responses often depend on the mechanical properties, pattern structures, and surface chemistry of the microenvironment surrounding the cells [1,3,4,5,7,8,10,11,12,13,14,15,16,17,18,19,20]; however, work done thus far has been conducted with structures composed of monolithic materials having uniform surface chemical composition. Studies of cell behavior on nanocomposite surfaces are limited [1,3,17,18,19,20].…”

Section: Introductionmentioning

confidence: 99%

Pattern-Dependent Mammalian Cell (Vero) Morphology on Tantalum/Silicon Oxide 3D Nanocomposites

et al. 2018

View full text Add to dashboard Cite

The primary goal of this work was to investigate the resulting morphology of a mammalian cell deposited on three-dimensional nanocomposites constructed of tantalum and silicon oxide. Vero cells were used as a model. The nanocomposite materials contained comb structures with equal-width trenches and lines. High-resolution scanning electron microscopy and fluorescence microscopy were used to image the alignment and elongation of cells. Cells were sensitive to the trench widths, and their observed behavior could be separated into three different regimes corresponding to different spreading mechanism. Cells on fine structures (trench widths of 0.21 to 0.5 μm) formed bridges across trench openings. On larger trenches (from 1 to 10 μm), cells formed a conformal layer matching the surface topographical features. When the trenches were larger than 10 μm, the majority of cells spread like those on blanket tantalum films; however, a significant proportion adhered to the trench sidewalls or bottom corner junctions. Pseudopodia extending from the bulk of the cell were readily observed in this work and a minimum effective diameter of ~50 nm was determined for stable adhesion to a tantalum surface. This sized structure is consistent with the ability of pseudopodia to accommodate ~4–6 integrin molecules.

show abstract

Section: Introductionmentioning

confidence: 99%

Pattern-Dependent Mammalian Cell (Vero) Morphology on Tantalum/Silicon Oxide 3D Nanocomposites

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The micro‐ and/or nanoscale topography have been known to affect cellular behaviors through changing the focal adhesion (FA) formation inside the cells with varying sizes, strength, and composition, which lead to realign of the cytoskeleton and change of cellular contractility. [ 41–43 ] During the process, the activation of the focal adhesion kinase (FAK), a key regulator of the mechanotransduction, has been reported in response to the various surface topography. [ 43 ] The downstream signaling of the FAK, such as ERK1/2 signal, has been known to affect the proliferation of the cells, [ 44,45 ] which might be involved in the increased proliferation of the hASCs in response to the various SIMs, SINs, and SIMNs suggested in the present study.…”

Section: Resultsmentioning

confidence: 99%

Micro/Nano Dual‐Scale Crossed Sinusoidal Wavy Patterns for Synergistic Promotion of Proliferation and Endothelial Differentiation of Human Adipose‐Derived Stem Cells

Kim

Lee

Park

et al. 2020

Adv Materials Inter

View full text Add to dashboard Cite

Although human adipose‐derived stem cells (hASCs) have great potential as a cell source for stem cell therapy due to their capacity of self‐renewal, long‐term growth, and multipotency with high obtainability through lipoaspiration, little is known about the effects of micro/nano dual‐scale topography on the proliferation and differentiation of the hASCs. In this study, the effects of micro/nano dual‐scale crossed sinusoidal wavy patterns (SIMNs) on the hASC behaviors including cellular alignment, proliferation, and endothelial differentiation are investigated. Various types of microscale, nanoscale, and SIMNs are fabricated on a single polystyrene surface in a step‐gradient manner based on the combined fabrication processes of deep X‐ray lithography, polydimethylsiloxane surface wrinkling, and thermal nanoimprinting process. The cellular alignment, proliferation, and endothelial differentiation of the hASCs are promoted on the SIMNs compared to the microscale or nanoscale sinusoidal wavy patterns. A micro/nano dual‐scale pattern of SIMN160_500, which has a micro‐scale sinusoidal wavy pattern with a wavelength of 160 µm crossed with a nanoscale sinusoidal wavy pattern with a wavelength of 500 nm, is found to be a most effective pattern for synergistically promoting both proliferation and endothelial differentiation of the hASCs with 1.2‐fold and 2‐fold increases, respectively.

show abstract

“…Although the spatial limitations of the nanopillars prevented the maturation of focal complexes to focal adhesions, cells exerted considerable force on the 200 nm-nanopillars, leading to bending of the nanopillars to form larger focal adhesions from adjacent focal complexes. Similarly NIH 3T3 fibroblasts cultured on PMMA nanopillars with a diameter of 70 nm formed very small adhesions, which led to a higher cells motility 61 .…”

Section: Discussionmentioning

confidence: 98%

Neuronal contact guidance and YAP signaling on ultra-small nanogratings

Tonazzini

Masciullo

Savi

et al. 2020

Sci Rep

View full text Add to dashboard Cite

contact interaction of neuronal cells with extracellular nanometric features can be exploited to investigate and modulate cellular responses. By exploiting nanogratings (nGs) with linewidth from 500 nm down to 100 nm, we here study neurite contact guidance along ultra-small directional topographies. the impact of nG lateral dimension on the neuronal morphotype, neurite alignment, focal adhesion (fA) development and YAp activation is investigated in nerve growth factor (nGf)differentiating PC12 cells and in primary hippocampal neurons, by confocal and live-cell total internal reflection fluorescence (TIRF) microscopy, and at molecular level. We demonstrate that loss of neurite guidance occurs in NGs with periodicity below 400 nm and correlates with a loss of FA lateral constriction and spatial organization. We found that YAP intracellular localization is modulated by the presence of nGs, but it is not sensitive to their periodicity. nocodazole, a drug that can increase cell contractility, is finally tested for rescuing neurite alignment showing mild ameliorative effects. Our results provide new indications for a rational design of biocompatible scaffolds for enhancing nerveregeneration processes.With its chemical and physical complexity, the extracellular environment can drive cell morphogenesis, migration and differentiation 1-4 , In particular, the contact interaction of cells with extracellular nanometric features has a primary role in regulating many physiological 5-12 and pathological processes 13 in vivo, and can be exploited to modulate cell responses in vitro [14][15][16][17] . Cells gather morphological information about the Extracellular matrix (ECM) through integrin-mediated adhesion clusters, called the focal adhesions (FAs), which act as topographical sensors. FAs can integrate multiple nanotopographical details into specific biomolecular instructions via the cytoskeletal signaling, which lastly regulates cell contractility, morphology and migration 9,18,19 , Artificial scaffolds with controlled micro/nano-topographies are definitively the apparatus of choice to better understand the response mechanisms of cells to external stimuli. They indeed allow to select and finely tune specific topographical aspects and selectively study their interaction with living system in vitro. The scientific literature reports many examples of nanostructures that can, for example, direct stem cell fate 20-22 , neuronal and glial adhesion 23-25 , differentiation 26 , polarization, and neurite orientation 14,18 , Our previous studies have demonstrated that plastic nanogratings (NGs) (i.e. alternating lines of sub-micron grooves and ridges, in the range between 500 and 2000 nm in linewidth) can promote neurite alignment and bipolarity of PC12 neuronal cells upon administration of nerve grow factor (NGF), simply by the contact guidance mechanism 27,28 , In these studies, adhesion on the ridges imposes a geometrical and directional constraint to FAs that results in neuronal polarization via the ROCK-mediated pathway 14,29-31 ...

show abstract

Correlation of focal adhesion assembly and disassembly with cell migration on nanotopography

Cited by 41 publications

References 46 publications

Pattern-Dependent Mammalian Cell (Vero) Morphology on Tantalum/Silicon Oxide 3D Nanocomposites

Pattern-Dependent Mammalian Cell (Vero) Morphology on Tantalum/Silicon Oxide 3D Nanocomposites

Micro/Nano Dual‐Scale Crossed Sinusoidal Wavy Patterns for Synergistic Promotion of Proliferation and Endothelial Differentiation of Human Adipose‐Derived Stem Cells

Neuronal contact guidance and YAP signaling on ultra-small nanogratings

Contact Info

Product

Resources

About