Correlation of EPHX1, GSTP1, GSTM1, and GSTT1 genetic polymorphisms with antioxidative stress markers in chronic obstructive pulmonary disease

Lakhdar, Ramzi; Denden, Sabri; Mouhamed, Manel Haj; Chalgoum, Abdelkader; Leban, Nadia; Knani, J.; Lefranc, Gérard; Miled, A.; Chibani, Jemni Ben; Khelil, Amel Haj

doi:10.3109/01902148.2010.535093

Cited by 33 publications

(33 citation statements)

References 48 publications

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“…Hence it is suggested that there may be a strong association of GST with lung function at the molecular level [44][45][46][47][48]. Interestingly, in this investigation, we also found GST activity was not only lower in COPD but also progressively decreased with the severity of COPD disease.…”

Section: Discussionsupporting

confidence: 71%

Altered antioxidant enzyme activity with severity and comorbidities of chronic obstructive pulmonary disease (COPD) in South Indian population

Asimuddin

Gutta

Ansari³

et al. 2017

COPD Res Pract

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Background: Oxidative stress has been suggested in the pathogenesis of Chronic Obstructive Pulmonary Disease (COPD) with an additional burden of diabetes, hypertension and cardiovascular disease. In the present study, we investigated erythrocyte antioxidant enzymes activities in correlation to COPD severity and COPD comorbidities. Methods: One hundred twenty seven subjects with COPD and 59 healthy controls participated in this study. COPD severity was done based on the Global Initiative for Chronic Obstructive Lung Disease criteria. The erythrocytes enzyme activities of superoxide dismutase (SOD), catalase (CAT), glutathione-s-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR) and total antioxidant status (TAS) were measured with spectrophotometric method. Results: COPD patients showed significant decrease in TAS (p > 0.05), GST (p < 0.001) and GPx (p < 0.01) activities with progression of the disease. In patients, FEV 1 was negatively correlated with SOD, GR and positively correlated with GST and GPx activities. Further, multivariate logistic regression analysis revealed GST (OR = 0.93; 95% CI = -0.10 -0.01; p < 0.01) , GPx (OR = 0.98; 95% CI = -0.03 -0.00; p < 0.05) and TAS (OR = 0.95; 95% CI = -0.08 -0.00; p < 0.05) were independently associated with FEV 1 in GOLD stage IV and GST (OR = 1.11; 95% CI = 0.04-0.18; p < 0.001) in GOLD stage II. Regression analysis confirmed a significant difference in GPx activity in COPD -type 2 diabetes (OR = 0.04; 95% CI = -6.60 -0.53; P = 0.09), and GST activity in COPD -cardiovascular disease (OR = 2.51; 95% CI = 0.00 -1.84; p < 0.05) patients when compared to patients without comorbidities. Conclusion: A significant decline in lung function may be associated with altered antioxidant enzyme activity due to the strong correlation between GST and GPx with COPD severity. Our results also indicate that erythrocytes GST and GPX activities are significantly associated with comorbidities, but only in COPD patients with type 2 diabetes and cardiovascular disease.

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Section: Discussionsupporting

confidence: 71%

Altered antioxidant enzyme activity with severity and comorbidities of chronic obstructive pulmonary disease (COPD) in South Indian population

Asimuddin

Gutta

Ansari³

et al. 2017

COPD Res Pract

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“…These results were consistent with Sahar S. Bessa et al's study in Egyptian patients with essential hypertension [29], and Lakhdar R. et al's study in the patients with chronic obstructive pulmonary disease [30]. Not as we observed in healthy young adults (in them the GST activity performing as GSTM1 genotype dependent) [31], GSTT1 deletion significantly increased the GST activity ( P = 0.034, <0.05) in the elderly.…”

Section: Discussionsupporting

confidence: 92%

Effects of GSTM1/GSTT1 Gene Polymorphism and Fruit & Vegetable Consumption on Antioxidant Biomarkers and Cognitive Function in the Elderly: A Community Based Cross-Sectional Study

Yuan

Liu

et al. 2014

PLoS ONE

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BackgroundIt was reported that Glutathione S-transferase (GST) gene polymorphism and fruit and vegetable (FV) intake were associated with body antioxidant capacity. The oxidative/anti-oxidative imbalance played an important role in the pathogenesis of AD. However, the association of GST genotype, dietary FV consumption with body antioxidant biomarkers and cognitive function in the elderly is not clear.ObjectiveThe aim of the present study was to determine the association of GST genotype, and dietary FV intake, with antioxidant biomarkers and cognitive function in the elderly.MethodsFood frequency questionnaire was used to collect data of dietary FV intakes in 504 community dwelling elderly aged from 55 to 75 years old. GSTM1 and GSTT1 genotypes were determined by using multiple-PCR method. Plasma and erythrocyte antioxidant biomarkers were measured. Cognitive function was measured by using Montreal Cognitive Assessment. Statistical analysis was applied for exploring the association of GST genotype and FV intake with antioxidant biomarkers level and cognitive function in the elderly.ResultsIndividual GSTM1 or GSTT1 gene deletion affects body antioxidant biomarkers levels, including erythrocyte GST activity, plasma total antioxidant capacity, and glutathione levels. GSTM1and/or GSTT1 gene deletion have no effects on cognitive function in the surveyed participants. The effect of GST genotype on antioxidant biomarkers are FV intake dependent. There is interaction of FV intake and GST genotype on cognitive function in the elderly.ConclusionGST genotype or daily FV consumption impact body antioxidant biomarkers, but not cognitive function in the elderly. There were combined effects of GST genotype and FV consumption on cognitive function in the elderly population. Large scale perspective population study is required to explore the association of GST genetic polymorphism, FV consumption and antioxidant biomarkers and cognitive function in the elderly.

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“…He et al (11) showed the combination of GSTT1 null genotype and GSTM1 and GSTP1 risk genotypes to be related to lung function impairments, while Lakhdar et al (33) concluded that combined GSTM1, GSTT1, GSTP1 and EPHX1 genetic polymorphisms may have favourable effects on redox balance in COPD patients. Rodriguez et al (14) found the modulating role of GSTP1 in COPD to take effect only in smokers lacking AAT.…”

Section: Gstp1 Gstm1 and Gstt1 Genetic Polymorphismsmentioning

confidence: 99%

GSTP1, GSTM1 and GSTT1 genetic polymorphisms and total serum GST concentration in stable male COPD

Žuntar¹,

Petlevski²,

Dodig³

et al. 2014

Acta Pharmaceutica

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Chronic obstructive pulmonary disease (COPD) is a common preventable and treatable disease characterized by persistent airflow limitation, which is usually progressive and associated with an enhanced chronic inflammatory response to noxious particles or gases in the airways and the lung. COPD is a leading cause of morbidity and mortality worldwide and is more prevalent in smokers, in those over 60 years of age and in men more than in women (1).Cigarette smoking is the best-studied COPD risk factor (2), but not all people with the same smoking history will develop COPD, due to differences in genetic predisposition to the disease. The best documented genetic risk factor is deficiency of alpha-1- The aim of this study was to test the hypothesis that glutathione-S-transferase (GST) genotypes were associated with COPD. GSTP1, GSTM1 and GSTT1 genotypes were determined by DNA methods and GST activity spectrophotometrically in older male Caucasian Croats (non--smokers, ex-smokers, and smokers) with stable COPD (n = 30) and sex/age matched controls (n = 60). The distribution of GSTP1 genotypes and alleles in controls vs. COPD showed a statistical difference (p < 0.05). The odds ratio of CC/CT+TT (wild type GSTP1 exon 6 vs. joint heterozygous and mutant homozygous GSTP1 exon 6) was 10.000 and statistically different (p = 0.002). In this study, the GSTP1 mutant genotype of exon 5 (GG), as well as GSTP1 mutant and heterozygous genotypes of exon 6 (TT and CT), were suggested to be genetic contributors to COPD susceptibility. Null GSTM1, null GSTT1 and joint GSTM1/GSTT1 null genotypes were not disease associated. Serum GST was not associated with GST genotypes and COPD or smoking history in our study subjects. Conclusions drawn from the study should be further supported and clarified by studies with larger sample sizes.

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Correlation of EPHX1, GSTP1, GSTM1, and GSTT1 genetic polymorphisms with antioxidative stress markers in chronic obstructive pulmonary disease

Cited by 33 publications

References 48 publications

Altered antioxidant enzyme activity with severity and comorbidities of chronic obstructive pulmonary disease (COPD) in South Indian population

Altered antioxidant enzyme activity with severity and comorbidities of chronic obstructive pulmonary disease (COPD) in South Indian population

Effects of GSTM1/GSTT1 Gene Polymorphism and Fruit & Vegetable Consumption on Antioxidant Biomarkers and Cognitive Function in the Elderly: A Community Based Cross-Sectional Study

GSTP1, GSTM1 and GSTT1 genetic polymorphisms and total serum GST concentration in stable male COPD

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