Correlation of different spectral lights with biomass accumulation and production of antioxidant secondary metabolites in callus cultures of medicinally important Prunella vulgaris L.

Fazal, Hina; Abbasi, Bilal Haider; Ahmad, Nisar; Ali, Syed Shujait; Akbar, Fazal; Kanwal, Farina

doi:10.1016/j.jphotobiol.2016.03.008

Cited by 92 publications

(71 citation statements)

References 34 publications

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“…Further investigation verified that a significant increase occurred in the total flavonoid content in the scions ( Figure 6A ), which suggested that graft union formation increases the content of a series of downstream antioxidant secondary metabolites by activating flavonoid biosynthesis. A number of studies have reported that a close relationship exists between the production of antioxidant secondary metabolites and callus formation (Fazal et al, 2016a,b). The accumulation of flavonoids may positively affect the grafting process by enhancing callus formation.…”

Section: Discussionmentioning

confidence: 99%

Comparative Proteomic Analysis of the Graft Unions in Hickory (Carya cathayensis) Provides Insights into Response Mechanisms to Grafting Process

Yuan

Tong

et al. 2017

Front. Plant Sci.

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Hickory (Carya cathayensis), a tree with high nutritional and economic value, is widely cultivated in China. Grafting greatly reduces the juvenile phase length and makes the large scale cultivation of hickory possible. To reveal the response mechanisms of this species to grafting, we employed a proteomics-based approach to identify differentially expressed proteins in the graft unions during the grafting process. Our study identified 3723 proteins, of which 2518 were quantified. A total of 710 differentially expressed proteins (DEPs) were quantified and these were involved in various molecular functional and biological processes. Among these DEPs, 341 were up-regulated and 369 were down-regulated at 7 days after grafting compared with the control. Four auxin-related proteins were down-regulated, which was in agreement with the transcription levels of their encoding genes. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the ‘Flavonoid biosynthesis’ pathway and ‘starch and sucrose metabolism’ were both significantly up-regulated. Interestingly, five flavonoid biosynthesis-related proteins, a flavanone 3-hyfroxylase, a cinnamate 4-hydroxylase, a dihydroflavonol-4-reductase, a chalcone synthase, and a chalcone isomerase, were significantly up-regulated. Further experiments verified a significant increase in the total flavonoid contents in scions, which suggests that graft union formation may activate flavonoid biosynthesis to increase the content of a series of downstream secondary metabolites. This comprehensive analysis provides fundamental information on the candidate proteins and secondary metabolism pathways involved in the grafting process for hickory.

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Section: Discussionmentioning

confidence: 99%

Comparative Proteomic Analysis of the Graft Unions in Hickory (Carya cathayensis) Provides Insights into Response Mechanisms to Grafting Process

Yuan

Tong

et al. 2017

Front. Plant Sci.

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“…The powder of dried spica (1.0 g) was mixed with 10 mL of 80% ethanol at 90°C in a water bath and stirred for 1.5 h. The total phenolic content was determined by the modified methods of Fazal et al (2016). Briefly, 0.2 mL of the aforementioned extracted solution was diluted with 4 mL of 70% ethanol.…”

Section: Total Phenolic Contentsmentioning

confidence: 99%

“…The total flavonoid contents were determined using the colorimetric method with modifications (Fazal et al 2016). The dried spica powder (1.0 g) was extracted with 10 mL of 35% ethanol at 86°C in a water bath and stirred for 3.5 h. One milliliter of the abovementioned extract solution was mixed with 0.7 mL of 50 mg mL −1 NaNO 3 in a test tube for 7 min.…”

Section: Total Flavonoid Contentsmentioning

confidence: 99%

Short-term UV-B radiation effects on morphology, physiological traits and accumulation of bioactive compounds in Prunella vulgaris L.

Zhang

Chen

et al. 2017

Journal of Plant Interactions

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Prunella vulgaris plants (full-bloom stage) were subjected to short-term (15 days) UV-B radiation in a growth chamber. UV-B radiation was effective at enhancing morphological and biomass characteristics and decreasing chlorophyll contents of P. vulgaris. Treatment of P. vulgaris with artificial UV-B radiation significantly increased peroxidase (POD), superoxide dismutase (SOD) and glutathione (GSH) activities compared to the control treatment. UV-B radiation significantly increased the levels of hydrogen peroxide (H 2 O 2 ), malondialdehyde (MDA) and proline in leaves of P. vulgaris compared to those of control plants. In addition, the contents of total flavonoids, rosmarinic acid, caffeic acid and hyperoside significantly increased under UV-B radiation. The total phenolic levels also increased under UV-B treatment. These results demonstrated that short-term UV-B radiation can enhance production of secondary metabolites in P. vulgaris, resulting in increased spica yield compared to that of control plants. ARTICLE HISTORY

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“…Fazal et al (2016) obtained no significant differences for the antioxidant activity in P. vulgaris callus. The difference between the amount and growth regulators used on the induction of callus modify the antioxidant activity (Bonfil et al, 2014).…”

Section: Antioxidant Activitymentioning

confidence: 75%

“…The in vitro culture of plant cells presents advantages for the supply of bioactive compounds with defined production system and short culture periods, ensures a continuous supply of metabolites of interest, and the cells are free of diseases and are not exposed to seasonal variations (Castro et al, 2016). Manipulations of in vitro nutritional components and physical factors are the main factors for the culture productivity optimization (Fazal et al, 2016). Studies on the chemical composition, antioxidant activity and volatile compounds of mangaba are scarce (Lima et al, 2015).…”

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confidence: 99%

Antioxidant Activity, Rutin Content and Genetic Similarity Between Matrices and Progenies of Hancornia speciosa

Machado¹,

Oliveira²,

Oliveira³

et al. 2018

JAS

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Mangaba tree (Hancornia speciosa Gomes) is a tropical fruit species from Brazil and presents socioeconomic potential. The objective of this study was to determine antioxidant activity, rutin content and genetic similarity among in vivo and in vitro matrices and progenies of six accessions from the Mangaba Active Germplasm Bank of the Embrapa Coastal Tablelands, SE, Brazil. Young leaves of adult matrix plants and in vitro callus methanolic extracts obtained from young leaves resulted in differences between the accessions for the rutin content and high antioxidant activity. The Costa Azul accession, from Bahia, Brazil outstood with values above 3,000 g of dry extract/g of DPPH (2.2-diphenyl-1-picrilhydrazyl) in in vitro callus coming from nodal and internodal segments (3,023.73 and 3,136.80 g of dry extract/g of DPPH, respectively). Rutin was not found in extracts resulting from in vitro callus of mangaba tree. The difference among the values obtained for DPPH analysis are superior when compared with in vivo leaf extract, with the in vitro Costa Azul accession (nodal and internodal) outstanding, concluding that in vitro callus induction can interfere with chemical compounds of the plant. The markers detect genetic similarity under in vitro cultivation conditions. The Costa Azul accession group itself in isolation from others and EC 50 concentrations differentiated between leaf and callus are obtained. O EC 50 is superior in extracts deriving from in vitro callus, with the in vitro Costa Azul accession (nodal and internodal).

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Correlation of different spectral lights with biomass accumulation and production of antioxidant secondary metabolites in callus cultures of medicinally important Prunella vulgaris L.

Cited by 92 publications

References 34 publications

Comparative Proteomic Analysis of the Graft Unions in Hickory (Carya cathayensis) Provides Insights into Response Mechanisms to Grafting Process

Comparative Proteomic Analysis of the Graft Unions in Hickory (Carya cathayensis) Provides Insights into Response Mechanisms to Grafting Process

Short-term UV-B radiation effects on morphology, physiological traits and accumulation of bioactive compounds in Prunella vulgaris L.

Antioxidant Activity, Rutin Content and Genetic Similarity Between Matrices and Progenies of Hancornia speciosa

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