Correlation of Cytohistlogical Expression and Serum Level of Ca125 in Ovarian Neoplasm

Das, Chhanda; Mukhopadhyay, Madhumita; Ghosh, T. K.; Saha, Ashis Kumar; Sengupta, Moumita

doi:10.7860/jcdr/2014/6689.4101

Cited by 5 publications

(7 citation statements)

References 19 publications

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“…[9] Chhanda Das et al reported 5 to 62 years in their study of 50 cases of ovarian neoplasms which was similar to other studies. [6,10] Most benign tumors occurred in the age range of 21 to 50 years that was consistent with other studies. [11] The peak incidence for malignancy in surface epithelial tumors was between 41 to 50 years of age.…”

Section: Discussionsupporting

confidence: 88%

“…[27,28] Study done by Chhanda Das et al had showed 19 (38%) cases with elevated serum levels, two of which were benign. [6] Immunohistochemistry CA 125-Preoperative serum CA 125 levels were elevated in 15 cases. Immunohistochemistry was performed in all the malignant & borderline tumors & also in those cases where serum levels were elevated.…”

Section: Discussionmentioning

confidence: 99%

“…Hence ample sampling of tissue may help reduce the disparity. [5,6] Immunohistochemistry for CA 125 may be useful in poorly differentiated serous carcinoma in establishing the diagnosis.…”

Section: Discussionmentioning

confidence: 99%

“…2+ = moderate positivity in 30 -80 % of tumor cells 3+ = intense positivity in > 80 % of tumor cells. [6,7] The histopathological findings were correlated with the serum levels & tissue expression of CA 125.…”

Section: Methodsmentioning

confidence: 99%

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Immunohistochemical Study of CA 125 in Surface Epithelial Tumors of Ovary in Correlation with Serum Levels

Pillarisetty

Ravindra

2020

Ann of Pathol and Lab Med

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Background: Ovarian carcinoma is the 4th leading cancer among women in India. Primary ovarian neoplasms exhibit a wide range of histopathological patterns and tumors with epithelial differentiation are most frequent. Among malignant tumors, most common histological type is serous adenocarcinoma whose diagnosis is established in advanced stages of disease in approximately 75% of patients. The most widely used tumor marker in ovarian cancer, often considered “gold standard” is Cancer Antigen125. Cancer Antigen 125 is a high molecular weight glycoprotein which is raised in approximately 90% of patients with advanced epithelial ovarian cancer. Methods: A 2 year prospective study included 81 cases of ovarian neoplasms with surface epithelial differentiation. The specimens were fixed in 10% formalin, routinely processed. Sections of 4-5 microns thickness were obtained from the paraffin block and stained with Hematoxylin & Eosin. The tumors were categorised according to WHO classification. Immunohistochemical analysis of Cancer Antigen 125 was done in all malignant & borderline tumors. Result: A total of 81 cases were studied. There were 15 cases with elevated serum Cancer Antigen 125 levels. Of these 8 showed positive tissue expression. The sensitivity of serum Cancer Antigen 125 was 68.75% & its specificity was 93.8%. Conclusion: Serum Cancer Antigen 125 is elevated in ovarian tumors especially in malignant surface epithelial tumors & more commonly in serous cystadenocarcinoma. There was a good correlation between serum levels & tissue expression of Cancer Antigen 125.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

“…Hence ample sampling of tissue may help reduce the disparity. [5,6] Immunohistochemistry for CA 125 may be useful in poorly differentiated serous carcinoma in establishing the diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Immunohistochemical Study of CA 125 in Surface Epithelial Tumors of Ovary in Correlation with Serum Levels

Pillarisetty

Ravindra

2020

Ann of Pathol and Lab Med

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“…The most widely used tumor marker in ovarian carcinoma; often considered the gold standard, is CA125. 1 It was first identified in 1981 as a high molecular weight glycoprotein by the murine monoclonal antibody, OC125. CA125 is not expressed on the surface epithelium of normal fetal and adult ovaries thought to be derived from coelomic epithelium.…”

Section: Introductionmentioning

confidence: 99%

Quantum Dot-labeled Tags Improve Minimal Detection Limit of CA125 in Ovarian Cancer Cells and Tissues

Shojaeian¹,

Allameh²,

Jeddi‐Tehrani³

et al. 2018

IJAAI

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In recent years, a lot of attention has been paid to quantum dot (QD) nanoparticles as fluorescent sensors for sensitive and accurate detection of cancer biomarkers. Here, using a homemade specific monoclonal antibody against CA125 and QD525- or FITC-labeled probes, expression of this marker in an ovarian cancer cell line and cancer tissues were traced and optical properties of fluorophores were compared qualitatively and quantitatively. Our results clearly showed that besides lower background and exceptionally higher photobleaching resistance, QD525 exhibited higher fluorescent intensity for both ovarian cancer cell and tissues at different exposure times (p<0.0001) and excitation filter sets (p<0.0001) exemplified by significantly higher staining index (p<0.016). More importantly, the FITC-labeled probe detected antigen-antibody complex at minimum concentration of 0.3 mg/mL of anti-CA125, while reactivity limit decreased to 0.078 mg/mL of anti-CA125 when QD525-labeled probe was applied showing four times higher reactivity level of QD525 probe compared to the same probe labeled with FITC. Based on our results, it seems that QDs are inimitable tags for sensitive detection and localization of ovarian cancer micrometastasis and molecular demarcation of cancer tissues in surgical practice, which subsequently figure out accurate therapeutic approaches.

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Polymer-Based Terbium Complex as a Fluorescent Probe for Cancer Antigen 125 Detection: A Promising Tool for Early Diagnosis of Ovarian Cancer

Mohamed,

Gamal,

El-Didamony

et al. 2024

ACS Omega

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A novel photoprobe, Tb–acetylacetone (Tb–ACAC) doped within a modified epoxy cellulose polymer immobilized with CA-125 monoclonal antibody, offers an accurate and highly selective method for early ovarian cancer (OC) diagnosis by detecting cancer antigen 125 (CA-125) in serum samples. This approach leverages quenching of the Tb–ACAC luminescence upon binding to CA-125. Characterization of the photoprobe film through UV–vis and fluorescence measurements confirmed the presence of Tb–ACAC within the polymer matrix. In aqueous solution (pH 6.8, λex = 365 nm), the characteristic emission band of Tb–ACAC at λem = 546.2 nm exhibited significant quenching upon CA-125 binding. This quenching effect enabled the sensitive and specific detection of CA-125 in diverse serum samples from OC patients, demonstrating the applicability, simplicity, and effectiveness of this novel approach.

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Correlation of Cytohistlogical Expression and Serum Level of Ca125 in Ovarian Neoplasm

Cited by 5 publications

References 19 publications

Immunohistochemical Study of CA 125 in Surface Epithelial Tumors of Ovary in Correlation with Serum Levels

Immunohistochemical Study of CA 125 in Surface Epithelial Tumors of Ovary in Correlation with Serum Levels

Quantum Dot-labeled Tags Improve Minimal Detection Limit of CA125 in Ovarian Cancer Cells and Tissues

Polymer-Based Terbium Complex as a Fluorescent Probe for Cancer Antigen 125 Detection: A Promising Tool for Early Diagnosis of Ovarian Cancer

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