Correlation of CD34⁺ Cells with Tissue Angiogenesis after Traumatic Brain Injury in a Rat Model

Abstract

Increasing evidence suggests that circulating endothelial progenitor cells, which are a subpopulation of hematopoietic progenitor CD34(+) cells, play a critical role in neovascularization and tissue repair. We have tested the hypothesis that traumatic brain injury (TBI) could mobilize CD34(+) cells to peripheral blood and brain tissue, a process critical for vascular repair, in a rat model of TBI. Male Wistar rats were subjected to controlled fluid percussion. Blood and brain tissue were collected before and after TBI to measure the levels of CD34(+) cells in peripheral blood and to detect their accumulation in the damaged cerebral tissue. Compared with surgery controls, CD34(+) cells significantly increased in the peripheral blood and accumulated in the brain tissue of TBI rats. Immunohistochemistry detected new vessels with incomplete CD34(+) endothelial-like cell lining and an increased number of microvessels in the injured and surrounding tissue. The results demonstrate a close correlation between an increase in circulating CD34(+) cells in response to traumatic injury and angiogenesis in TBI rat brain. They also suggest that transplantation of CD34(+) cells or augmentation of endogenous CD34(+) cells may be a novel therapeutic approach for patients with TBI.

Expression of ephrinB2 and EphB4 in a neonatal rat model of periventricular white matter damage

“…Our findings indicate that HI promotes CD34 + cell accumulation to form a microvascular system. Similar results have been observed in rats after traumatic brain injury [9]. Next, we focused on classifying the remodeled vessels.…”

VEGI attenuates the inflammatory injury and disruption of blood–brain barrier partly by suppressing the TLR4/NF-κB signaling pathway in experimental traumatic brain injury

“…A 2.0-mm hole was drilled on the right parietal skull (2.0 mm posterior from bregma and 1.5 mm lateral to the sagittal suture) to expose the dura. Mice were subjected to experimental FPI (model 01-B; New Sun Health Products, Cedar Bluff, VA) injury of severity of 2.0 atm, as described previously (Dixon et al, 1987;McIntosh et al, 1989 Q2 ; Guo et al, 2009;Zhang et al, 2013). In brief, a female Leur-Lok fitting was cemented to the craniotomy site (MODEL 01-B, New Sun, USA).…”

Early exercise improves cerebral blood flow through increased angiogenesis in experimental stroke rat model

“…Although it occurs during normal embryonic development, some insults such as brain trauma and ischemia induced angiogenesis and vascular remodeling in adult [12,13]. Within a few hours after occlusion, hypoxia induced up-regulated expression of a group of angiogenic factors in infarct hemisphere including vascular endothelial growth factor (VEGF), Ang1/2 and their receptor Tie2.…”