Correlation of bronchial eosinophil and mast cell activation with bronchial hyperresponsiveness in children with asthma

Ferguson, Alexander C.; Whitelaw, Mary; Brown, Huguette

doi:10.1016/0091-6749(92)90133-m

Cited by 123 publications

(77 citation statements)

References 22 publications

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“…It will be important to follow-up the one non-asthmatic atopic child who had a high BAL eosinophil count to see if he subsequently develops asthma symptoms. A previous study reported increased eosinophils in BAL fluid from older asthmatics/adolescents (8-17 years) but failed to demonstrate mast cells [6]. In our study, we used the glass coverslip technique [10,11] and specialized fixing and staining methods which enabled the enumeration of mast cells.…”

Section: Discussionmentioning

confidence: 99%

“…Plastic surgical procedures were the most common elective operations (n = 59) and overall the most common procedures were removal of skin or mucous membrane lesions (34), correction of prominent ears (24), urological interventions (17) and tonsils and adenoids (6). The lavage procedure took 1 min.…”

Section: Patient and Post-operative Datamentioning

confidence: 99%

“…It is probable that atopic children with asthma (atopic asthmatics, AA) and who suffer from frequent interval symptoms will have persisting airways inflammation [6]. However, many children wheeze predominantly in winter and only in association with a viral respiratory infection (viral associated wheeze, VAW) and have completely asymptomatic interval periods [7].…”

Section: Introductionmentioning

confidence: 99%

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Bronchoalveolar lavage findings suggest two different forms of childhood asthma

Stevenson¹,

Turner

Heaney³

et al. 1997

Clin Experimental Allergy

255

196

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SummaryBackground It seems plausible that children with atopy and persistent asthma symptoms will, like their adult counterparts, have chronic airways inflammation. However, many young children with no other atopic features have episodic wheezing that is triggered solely by viral respiratory infections. Little is known as to whether airways inflammation occurs in these two asthma patterns during relatively asymptomatic periods. Methods Using a non-bronchoscopic bronchoalveolar lavage (BAL) procedure on children presenting for an elective surgical procedure, this study has investigated the cellular constituents of BAL fluid in children with a history of atopic asthma (AA) non-asthmatic atopic children (NAA) or viral associated wheeze (VAW). Results A total of 95 children was studied: 52 with atopic asthma (8.0 years, range 1.1-15.3, 36 male), 23 with non-asthmatic atopy (median age 8.3 years, range 1.7-13.6, 11 male) and 20 with VAW (3.1 years, range 1.0-8.2, 13 male). No complications were observed during the lavage procedure and no adverse events were noted post-operatively. Total lavage fluid recovered was similar in all groups and the total cell numbers were higher in the VAW group. Eosinophil (P< 0.005) and mast cell (/'<0.05) numbers were significantly elevated in the group with atopic asthma. Conclusions During relatively asymptomatic periods there is on-going airways inflammation, as demonstrated by eosinophil and mast cell recruitment, in children with asthma and atopy but not in children with viral associated wheeze or atopy alone. This strongly suggests that there are different underlying pathophysiologicai mechanisms in these two groups of children who wheeze.

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Section: Discussionmentioning

confidence: 99%

Section: Patient and Post-operative Datamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bronchoalveolar lavage findings suggest two different forms of childhood asthma

Stevenson¹,

Turner

Heaney³

et al. 1997

Clin Experimental Allergy

255

196

View full text Add to dashboard Cite

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“…Among these, eosinophils are predominant effector cells for tissue damage and pulmonary dysfunction (5,6). The severity of AHR strongly correlates with the intensity of pulmonary infiltration by eosinophils (7)(8)(9).…”

mentioning

confidence: 99%

Eotaxin Represents the Principal Eosinophil Chemoattractant in a Novel Murine Asthma Model Induced by House Dust Containing Cockroach Allergens

Kim

Merry

Nemzek

et al. 2001

The Journal of Immunology

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Asthma represents a serious health problem particularly for inner city children, and recent studies have identified that cockroach allergens trigger many of these asthmatic attacks. This study tested the concept that asthma-like pulmonary inflammation may be induced by house dust containing cockroach allergens. An aqueous extract was prepared from a house dust sample containing endotoxin and high levels of cockroach allergens. BALB/c mice were immunized with the house dust extract (HDE) and received two additional pulmonary challenges. Bronchoalveolar lavage (BAL) eosinophil counts and eotaxin levels were significantly increased in immunized mice exposed to the HDE, whereas neutrophils were the predominant BAL inflammatory cell in the unimmunized mice. Kinetics studies in immunized mice demonstrated a peak pulmonary inflammatory response 48 h after the last challenge. The allergic response in this model was further confirmed by histological and physiological studies demonstrating a significant influx of eosinophils and lymphocytes in the peribronchial area, and severe airway hyperreactivity through whole-body plethysmography. The specificity of the response was established by immunizing with HDE and challenging with purified cockroach allergen, which induced pulmonary eosinophilia and airway hyperreactivity. Ab inhibition of eotaxin significantly inhibited the number of BAL eosinophils. These data describe a novel murine model of asthma-like pulmonary inflammation induced by house dust containing endotoxin and cockroach allergens and further demonstrate that eotaxin represents the principal chemoattractant for the recruitment of the pulmonary eosinophils.

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“…Indeed, several studies have shown that inflammatory changes occur in the airways of children with mild asthma. These changes assist in the recruitment of leukocytes, including eosinophils, and neutrophils [7][8][9][10][11][12]. Neutrophil recruitment into the airways of asthmatic children has been associated with the release of the potent neutrophil attractant interleukin (IL)-8 [13,14].…”

mentioning

confidence: 99%

Role of the chemokines RANTES, monocyte chemotactic proteins-3 and -4, and eotaxins-1 and -2 in childhood asthma

Rojas‐Ramos¹,

Avalos²,

Pérez-Fernandez³

et al. 2003

European Respiratory Journal

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Eosinophil recruitment into the airways is a feature of asthma in children. However, the mechanisms by which these cells migrate into the airways are not fully understood. The present study investigated the presence of the eosinophilactivating chemokines regulated on activation, normal T-cell expressed and secreted (RANTES), monocyte chemotactic proteins (MCP)-3 and -4, and eotaxins-1 and -2 in the bronchoalveolar lavage (BAL) fluid obtained from both asthmatic (n=10, age 6-10 yrs) and normal children (n=10, age 5-10 yrs).Measurements of chemokines in BAL fluid showed that levels of RANTES, MCPs-3 and -4, and eotaxins-1 and -2 were significantly increased in fluid obtained from asthmatic children when compared with normal children. Among the different chemokines, RANTES was the cytokine released in greatest quantities in BAL fluid from asthmatic children. There was a significant correlation between the concentrations of MCP-4 and eosinophil numbers in BAL fluid and a trend between both chemokines MCP-3 and eotaxin-2 and eosinophils.Interestingly, the levels of most chemokines correlated with one another. These findings suggest that RANTES monocyte chemotactic proteins-3 and -4, and eotaxins-1 and -2 may regulate eosinophil trafficking into the airways of asthmatic children in a coordinated manner. Eur Respir J 2003; 22: 310-316.

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Correlation of bronchial eosinophil and mast cell activation with bronchial hyperresponsiveness in children with asthma

Cited by 123 publications

References 22 publications

Bronchoalveolar lavage findings suggest two different forms of childhood asthma

Bronchoalveolar lavage findings suggest two different forms of childhood asthma

Eotaxin Represents the Principal Eosinophil Chemoattractant in a Novel Murine Asthma Model Induced by House Dust Containing Cockroach Allergens

Role of the chemokines RANTES, monocyte chemotactic proteins-3 and -4, and eotaxins-1 and -2 in childhood asthma

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