Correlation of blood-brain barrier function and HT7 protein distribution in chick brain circumventricular organs

Albrecht, U.; Seulberger, Harald; Schwarz, Heinz; Risau, Werner

doi:10.1016/0006-8993(90)91823-y

Cited by 55 publications

(33 citation statements)

References 22 publications

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“…This antigen is present in endothelial cells of chick brain capillaries which form the blood-brain barrier but is absent from the vascular system of the brain circumventricular organs which lack a barrier (Albrecht et al, 1990). During chick brain embryogenesis this antigen appears on brain vessels at embryonic day 10, just when the capillaries in the brain become tight to horseradish peroxidase.…”

Section: Introductionmentioning

confidence: 97%

“…Using monoclonal and polyclonal antibodies we have characterized one such molecule, the HT7 antigen Albrecht et al, 1990). This antigen is present in endothelial cells of chick brain capillaries which form the blood-brain barrier but is absent from the vascular system of the brain circumventricular organs which lack a barrier (Albrecht et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

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The inducible blood-brain barrier specific molecule HT7 is a novel immunoglobulin-like cell surface glycoprotein.

1990

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The unique properties of brain endothelial cells, which form the blood-brain barrier, are reflected by the expression of specific cell surface molecules. We report here the purification, cloning and expression of one such molecule which is recognized by HT7 monoclonal antibodies. The HT7 antigen is a highly glycosylated 45-52 kd protein localized in brain endothelial cells, kidney epithelial cells and erythroblasts. The protein was purified to homogeneity from plasma membrane proteins isolated from all three sources using immunoaffinity chromatography and reverse phase HPLC. The aminoterminal amino acid sequences of the proteins were found to be identical. Based on amino acid sequence information, specific primers were designed and the polymerase chain reaction was used to obtain a full length cDNA clone. The nucleotide sequence encoded a novel glycoprotein with two C2-like immunoglobulin related domains, one transmembrane domain and a cytoplasmic tail. Expression of the transfected cDNA in COS cells resulted in the appearance of the HT7 antigen on the surface of these cells. On the basis of our results we propose that the protein may be a receptor involved in cell surface recognition at the blood-brain barrier.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

The inducible blood-brain barrier specific molecule HT7 is a novel immunoglobulin-like cell surface glycoprotein.

1990

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“…The molecular weight of HT7/neurothelin is approximately 43 kDa (Schlosshauer and Herzog, 1990), which is almost the same as that of EMMPRIN expressed in human brain and gliomas in our study. Endothelial cells in the circumventricular organs that lack a blood-brain barrier, such as the pituitary, median eminence, subfornical organ, pineal gland and area postrema, were negative for HT7/neurothelin (Albrecht et al, 1990). These observations suggest a correlation between blood-brain barrier function and expression of HT7/neurothelin.…”

Section: Discussionmentioning

confidence: 50%

Role of Tumor Collagenase Stimulating Factor in Breast Cancer Invasion and Metastasis.

Zucker¹

1996

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Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources. gathering and maintaining the data needed, and completing and reviewing the collection of Information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway. Suite 1204, Arlington, VA 22202-4302. and to the Office of Management and Budget, Paperwork Reduction Project (0704-0188). Washington, DC 20503. AGENCY USE ONLY (Leave blank)2. REPORT DATE December 1999 REPORT TYPE AND DATES COVEREDFinal (1 Dec 94 -30 Nov 99) TITLE AND SUBTITLE Role of Tumor Collagenase Stimulating Factor in BreastCancer Invasion and Metastasis 6. AUTHOR(S) Stanley Zucker, M. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES)The SUPPLEMENTARY NOTES 12a. DISTRIBUTION / AVAILABILITY STATEMENTApproved for public release; distribution unlimited 12b. DISTRIBUTION CODE ABSTRACT (Maximum 200Extracellular matrix metalloproteinase inhibitor {EMMPRIN), a plasma membrane glycoprotein identified originally in carcinoma cells, is responsible for inducing peritumoral fibroblasts to produce matrix metalloproteinases (MMPs), thereby enhancing cancer invasion and metastasis. Using in situ hybridization and immunohistochemistry, we have identified EMMPRIN in breast cancer tissue. We have characterized the human EMMPRIN gene and have noted a high degree of conservation with the mouse gene. The effect of transfecting human breast cancer cells with EMMPRIN cDNA was explored. Expression of EMMPRIN cDNA by MDA-MB-436 cancer cells resulted in marked increase in tumor growth and invasiveness after tumor cell injection into mammary tissue of nude mice. Using the phage display system, crosslinking and ligand affinity chromatography, MMP-1 binding to EMMPRIN was identified suggesting the novel possibility that EMMPRIN binds MMP-1 to the tumor cell surface, thus enhancing pericellular degradation of extracellular matrix. The effect of EMMPRIN on endothelial MMPs was explored in vitro; EMMPRIN enhanced endothelial production of stromelysin-1, collagenase, and gelatinase A. These data support a role for EMMPRIN in enhancing cancer dissemination. Where copyrighted material is quoted, permission has been obtained to use such material. SUBJECT TERMSWhere material from documents designated for limited distribution is quoted, permission has been obtained to use the material. The purpose of this grant has been to determine the role of Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) in breast cancer invasion and metastasis. EMMPRIN is a glycoprotein identified on the plasma membrane of cancer cells which induces fibroblasts to produce Matrix Metalloproteinases (MMPs) (l).Originally known as Tumor Collagenase Stimulating Factor (TCSF) because it was described as a stimulator of inter...

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“…The HT7 antigen is specifically expressed on chicken BBB endothelium but not on other endothelial cells Albrecht et al, 1990). It is absent from the fenestrated choroid plexus endothelium but present on choroid plexus epithelium, the site of the cerebrospinal fluid barrier.…”

Section: Characterization Of the Blood-brain Barriermentioning

confidence: 94%

“…Risau and co-workers characterized a monoclonal antibody named HT7 that recognizes a highly glycosylated 48 kDa protein, which belongs to the immunoglobulin-superfamily, which is expressed specifically on cerebral endothelium (Albrecht et al, 1990;Seulberger et al, 1990;. The HT7 antigen is specifically expressed on chicken BBB endothelium but not on other endothelial cells Albrecht et al, 1990).…”

Section: Characterization Of the Blood-brain Barriermentioning

confidence: 99%

The seminal work of Werner Risau in the study of the development of the vascular system

Ribatti¹

2010

Int. J. Dev. Biol.

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Werner Risau's work had a decisive impact on defining the overall nature of neovascularization processes during development and had the capability to integrate different directions in the field of endothelial cell biology research. In particular, he had a special interest in understanding the development, differentiation and maintenance of the blood-brain barrier. Risau very successfully propagated the concept that the same factors, such as basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF), which are essential for the formation of blood vessels during embryonic development, also influence pathological angiogenesis during tumor growth. This review is dedicated to a retrospective analysis of the most important contributions of Risau's work in the field of angiogenesis during embryonic development and in post-natal life, in both physiological and pathological conditions.

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Correlation of blood-brain barrier function and HT7 protein distribution in chick brain circumventricular organs

Cited by 55 publications

References 22 publications

The inducible blood-brain barrier specific molecule HT7 is a novel immunoglobulin-like cell surface glycoprotein.

The inducible blood-brain barrier specific molecule HT7 is a novel immunoglobulin-like cell surface glycoprotein.

Role of Tumor Collagenase Stimulating Factor in Breast Cancer Invasion and Metastasis.

The seminal work of Werner Risau in the study of the development of the vascular system

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