Correlation of antistaphylococcal antibody titers with severity of staphylococcal disease

Jackson, L.; Sottile, Margaret; Aguilar-Torres, Francisco G.; Dee, Thomas H.; Rytel, Michael W.

doi:10.1016/0002-9343(78)90583-1

Cited by 31 publications

(13 citation statements)

References 9 publications

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“…When S. aureus has been isolated from blood cultures, the antibody response might be useful to discriminate between contamination of blood cultures and true infection and to differentiate between patients with endocarditis or secondary foci o f infection who need a longer duration of therapy as compared to patients with uncomplicated septicaemia. The results of the present anti-teichoic acid assay using cell-wall teichoic acid of s. aureus strain E 1369 were comparable with those of other similar assays using teichoic acid from different strains of S. aureus (2,8,12,18). The exact composition of teichoic acid of strain E 1369 has not been established.…”

Section: Discussionsupporting

confidence: 87%

“…Patients, with S. aureus endocarditis have previously showed positive teichoic acid antibodies in 59-100% (2,8,12,18) and in the present study 6 1 Yo were positive. The difficulties in differentiating complicated from uncomplicated septicaemia are well known from other anti-teichoic assays (2,8,12,18). Actually, this problem has best been overcome, when a crude extract of the protein A-deficient S. aureus strain Wood 46 was used as antigen in solid-phase radioimmuno-assay ( 1 7).…”

Section: Discussionsupporting

confidence: 70%

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ANTIBODY RESPONSE TO WHOLE CELLS AND TEICHOIC ACID OF STAPHYLOCOCCUS AUREUS STRAIN E 1369 IN HUMAN SERA

Jarløv

Espersen

Christensson

et al. 1987

Acta Pathologica Microbiologica Scandinavica Series B: Microbio

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A whole cell Staphylococcus aureus enzyme‐linked immunosorbent assay (ELISA) using the protein A‐deficient strain E 1369 as antigen was compared to an ELISA with cell‐wall teichoic acid, extracted from the same strain, as antigen for the detection of IgG antibodies against S. aureus in human sera. A total of 178 serum samples from 119 patients with S. aureus endocarditis, complicated and uncomplicated septicaemia, non‐S. aureus endocarditis and septicaemia were investigated together with sera from 155 healthy controls. The sensitivity in diagnosing S. aureus endocarditis was better for the whole cell‐ELISA (83%) as compared to the teichoic acid‐ELISA (61%), and the same was true for complicated septicaemia (41% vs 29%). The whole cell ELISA was also more specific for S. aureus infections with only 3.6% false positive test values compared to 7.1% for the teichoic acid‐ELISA. The differences were however, not statistically significant. None of the assays were able to distinguish between complicated and uncomplicated S. aureus septicaemia.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 70%

ANTIBODY RESPONSE TO WHOLE CELLS AND TEICHOIC ACID OF STAPHYLOCOCCUS AUREUS STRAIN E 1369 IN HUMAN SERA

Jarløv

Espersen

Christensson

et al. 1987

Acta Pathologica Microbiologica Scandinavica Series B: Microbio

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“…Blood specific antibody levels against a given pathogen is a classical marker of the infectious burden (21)(22)(23)(24)(25), frequently correlated with interindividual infection transmission, as shown for example in human T-cell virus type-1 infection for motherchild transmission (26,27). With respect to HHV-8, most studies investigating the correlation between antibody titers and the viral load were done in individuals coinfected with HIV often suffering from Kaposi's sarcoma (KS).…”

Section: Introductionmentioning

confidence: 99%

Respective Roles of Serological Status and Blood Specific Antihuman Herpesvirus 8 Antibody Levels in Human Herpesvirus 8 Intrafamilial Transmission in a Highly Endemic Area

et al. 2004

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Transmission of human herpesvirus 8 (HHV-8), the etiological agent of Kaposi's sarcoma, occurs mainly during childhood in endemic countries and, to a large extent, through intrafamilial contacts. To additionally investigate this familial transmission, and especially the role of plasma anti-HHV-8 antibody titers, we conducted a large survey in a village from Cameroon, Central Africa, including 92 families (608 individuals). Plasma samples were tested for specific IgG directed against HHV-8 lytic antigens by immunofluorescence assay, and titers were determined by 2-fold dilutions. Global HHV-8 seroprevalence was 60%, raising from 32% under 9 years up to a plateau of around 62% between 15 and 40 years. The familial correlation patterns in HHV-8 seropositive/seronegative status showed strong dependence from mother to child and between siblings. In contrast, no familial correlation in anti-HHV-8 antibody levels was observed among infected subjects. In particular, no relationship was observed between the anti-HHV-8 antibody titer of HHV-8 seropositive mothers and the proportion of their HHV-8 seropositive children. Furthermore, a random permutation study of the anti-HHV-8 antibody titers among HHV-8 infected subjects showed that the main risk factor for infection was the HHV-8 serologic status and not the antibody level. In addition, no correlation was found between anti-HHV-8 antibody levels and buffy coat HHV-8 viral loads in a subsample of 95 infected subjects. Overall, these results strongly suggest that, in this highly endemic population from Central Africa, HHV-8 transmission mainly occurs from mother to child and between siblings, and it is independent of plasma antibody levels of HHV-8 infected relatives.

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“…Previously, various authors had tested various cell wall components of .S' . au reus for immunogenicity and sérodiagnostic utility, such as protein A [19], PG [9,[20][21][22][23], and teichoic acid or lipoteichoic acid [13,14,22,[24][25][26][27][28][29][30][31][32][33][34][35][36]. Perousal of the literature indi cated that the enzyme-linked immunosor bent assay (ELISA) largely had replaced oth er methods such as Ouchterlony double dif fusion in agar [26], countercurrent immunoelectrophoresis [29], and the radioimmu noassay [37], Most important, none of the tests for antibodies against exoenzymes, exo toxins, or cell wall components was capable of separating patients with complicated in fection (e.g., endocarditis, septicemia, osteo…”

Section: Introductionmentioning

confidence: 99%

Immunobiology of Methicillin-Resistant <i>Staphylococcus aureus</i>: Immune Response of Rabbits and Patients to Systemic Infection

Traub

Bauer²,

Leonhard³

1996

Chemotherapy

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Teichoic acid (TA) and peptidoglycan (PG) extracted from Staphylococcus aureus strains ATCC 25923 and Lafferty as well as formalinized cells of these two strains and several clinical methicillin-resistant S. aureus (MRSA) isolates were immunogenic for New Zealand White rabbits. Rabbits which had recovered from experimental bacteremia due to MRSA seroconverted, i.e. demonstrated raised titers of antibodies against TA and PG of the S. aureus strain Lafferty and against whole cells (WC) and ultrasound cell lysates (UCL) of MRSA isolates No. 1 and 2 (representative of nosocomial MRSA strain I), as determind with enzyme-linked immunosorbent assays. Furthermore, sera from 2 long-term survivor rabbits recognized four polypeptides (apparent molecular weights = 38.9, 33.9, 30.9, and 28.2 kDa) shared by UCL extracts from MRSA isolates No. 1 and 2, as determined with immunoblots. Neither normal nor immune rabbit sera augmented the bactericidal activity of fresh defibrinated human blood (65% v/v) against selected MRSA isolates and S. aureus strain ATCC 25923. Sera from 12 patients with documented systemic infection due to MRSA outbreak strain I were examined for IgM and IgG antibodies against TA, WC, and UCL antigens. Three patient sera exhibited raised IgM antibodies against TA; 7 of 12 patient sera showed increased IgG anti-TA titers. Only 1 patient had a markedly raised IgM anti-WC titer, whereas 4 and 10 of the patients had increased IgG titers against WC from MRSA isolates No. 1 and 2, respectively. However, all 12 patients had raised IgG titers against UCL from MRSA isolate No. 2 versus 4 of 12 patients with elevated IgG titers against UCL from MRSA isolate No. 1. Immunoblots with 3 selected patient sera revealed IgG antibodies to be more multifaceted than IgM antibodies. Sera from 11 of the 12 patients contained antimicrobial drug(s); yet only 5 of these 11 sera (used at 10% v/v in broth) killed inocula of MRSA isolate No. 43. None of the 12 patient sera (10% v/v) enhanced the bactericidal activity of human blood against selected MRSA isolates. Neither three commercial intravenously applicable IgG preparations nor an IgG anti-α-hemolysin formulation (employed at 10% v/v) augmented the bactericidal activity of fresh defibrinated human blood against selected MRSA isolates comprising MRSA outbreak strain I.

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Correlation of antistaphylococcal antibody titers with severity of staphylococcal disease

Cited by 31 publications

References 9 publications

ANTIBODY RESPONSE TO WHOLE CELLS AND TEICHOIC ACID OF STAPHYLOCOCCUS AUREUS STRAIN E 1369 IN HUMAN SERA

ANTIBODY RESPONSE TO WHOLE CELLS AND TEICHOIC ACID OF STAPHYLOCOCCUS AUREUS STRAIN E 1369 IN HUMAN SERA

Respective Roles of Serological Status and Blood Specific Antihuman Herpesvirus 8 Antibody Levels in Human Herpesvirus 8 Intrafamilial Transmission in a Highly Endemic Area

Immunobiology of Methicillin-Resistant <i>Staphylococcus aureus</i>: Immune Response of Rabbits and Patients to Systemic Infection

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