Correlation of antibodies against desmogleins 1 and 3 with indirect immunofluorescence and disease status in a Greek population with pemphigus vulgaris

Abstract

Serum testing of anti-Dsg1 and anti-Dsg3 in PV patients not only provides significant correlations with IIF, treatment and disease type, but may serve as a monitoring tool for clinical course and treatment guidance.

The Value of Anti-Desmoglein Enzyme-Linked Immunosorbent Assay in the Immunological Follow-Up of Pemphigus

“…In contrast, other studies did not find a correlation during follow-up [9,16,20]. In our study, we observed a better but not significant association for Dsg3 than Dsg1 ELISA, which was also observed by Herrero-González et al [17], in contrast with four other studies which found a better correlation for Dsg1 ELISA [10,18,21,22]. …”

“…Although our study included a limited number of patients, we did not find any significant association of Dsg ELISA values and clinical status. Only a few previous studies with a similar or higher number of patients (from 40 to 63) compared to our study published results regarding the interest of Dsg ELISA during follow-up [8,9,10,11]. Dsg ELISA has >90% sensitivity and specificity at diagnosis [4,5,12], but studies show paradoxical results during follow-up [8,9].…”

“…However, the study population was Indian, having a higher frequency of Dsg1 antibodies than European patients [13]. Other studies showed a correlation between Dsg ELISA findings and clinical status during follow-up [10,11,14,15], but still others did not find a correlation between Dsg1 ELISA results and cutaneous severity [16,17] or Dsg3 ELISA results and mucosal severity [18,19], including disease activity scoring with arbitrary and non-validated clinical activity scores [9,16,17]. In contrast, other studies did not find a correlation during follow-up [9,16,20].…”

Autoantibody Levels and Clinical Disease Severity in Patients with Pemphigus: Comparison of Aggregated Anti-desmoglein ELISA Values and Indirect Immunofluorescence Titres

“…A correlation between ELISA scores and disease activity has been assessed in several studies and it was proposed that ELISA values tend to correlate with the severity of the disease (2,10,13,14,(16)(17)(18)(19)(20)(21)(22)(23)(24). More precisely, skin disease severity was initially suggested to be related to Dsg1 antibody levels, whereas Dsg3 antibody levels correlate directly with the involvement of mucous membranes (4,21).…”

Ung gutt med feber, sår hals, såre lepper og bulløst utslett

“…Dette kan dermed skille tilstanden fra dermatitis herpetiformis, der man ser IgA-nedslag i dermale papillae (9), og fra pemphigus vulgaris, der man finner karakteristiske IgG-avleiringer i epidermis (10). Ved bulløst pemfigoid ses nedslag langs basalmembranen (11 Histologiske funn og immunfluorescensfunn styrket mistanken om erythema multiforme.…”