Correlation of altered expression of a long non-coding RNA, NEAT1, in peripheral blood mononuclear cells with dengue disease progression

Pandey, AK; Goswami, Saptamita; Shukla, Shweta; Das, Shaoli; Ghosal, Suman; Pal, Manisha; Bandyopadhyay, Bhaswati; Ramachandran, V G; Basu, Nandita; Sood, Vikas; Pandey, Priyanka; Chakrabarti, Jayprokas; Vrati, Sudhanshu; Banerjee, Arup

doi:10.1016/j.jinf.2017.09.016

Cited by 26 publications

(32 citation statements)

References 30 publications

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“…Recent evidence indicates that lncRNA NEAT1 plays an important role in the transcriptional regulation of cytokines (such as: interleukin‐8 (IL‐8)) in a viral infection, and it stimulates the activation of several inflammasomes (such as NLRP3, NLRC4, and AIM2 inflammasomes), which leads to immune response in immunoinflammatory diseases . And the involvement of lncRNA NEAT1 in immune system has been explored in several diseases including sepsis, systemic lupus erythematosus (SLE), mild dengue, and herpes simples . For example, lncRNA NEAT1 expression in peripheral blood mononuclear cells is remarkably increased in sepsis patients than that in HCs, and ROC curve illustrates that lncRNA NEAT1 is of good value in predicting sepsis risk with AUC 0.851 (0.812‐0.935) .…”

Section: Discussionmentioning

confidence: 99%

Longnon‐coding RNA NEAT1 overexpression associates with increased exacerbation risk, severity, and inflammation, as well as decreased lung function through the interaction with microRNA‐124 in asthma

Lü

2019

Clinical Laboratory Analysis

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This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. AbstractBackground: This study aimed to explore the association of long non-coding RNA nuclear-enriched abundant transcript 1 (lncRNA NEAT1) with exacerbation risk, lung function, and inflammatory cytokines in asthma. Methods:A total of 170 patients with asthma in exacerbation, 170 patients with asthma in remission, and 170 healthy controls (HCs) were enrolled, and their plasma samples were collected. The expressions of lncRNA NEAT1 and microRNA-124 (miRNA-124) in plasma were detected by real-time quantitative polymerase chain reaction; inflammatory cytokines in plasma were measured by the Enzyme-linked immunosorbent assay (ELISA); and pulmonary ventilation function was detected by examination of forced expiratory volume in 1 second (FEV1) and forced vital capacity (FVC).Results: LncRNA NEAT1 expression was upregulated in asthma patients in exacerbation compared with HCs and asthma patients in remission, and receiver operating characteristic curve exhibited that it was of good value in distinguishing asthma patients in exacerbation from HCs (AUC: 0.869 (0.830-0.908)) and asthma patients in remission (AUC: 0.775 (0.724-0.825)). Furthermore, lncRNA NEAT1 was positively correlated with exacerbation severity, TNF-α, IL-1β, and IL-17, but negatively correlated with IL-10, FEV 1 /FVC and FEV 1 %predicted in asthma patients. Additionally, lncRNA NEAT1 was negatively correlated with miR-124, and miR-124 was negatively associated with exacerbation risk, exacerbation severity, and inflammation, but positively associated with lung function in asthma patients. Conclusion:Circulating lncRNA NEAT1 exhibits potential to be a new biomarker for elevated exacerbation risk and severity of asthma. K E Y W O R D S asthma, exacerbation, lncRNA NEAT1, miR-124 S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section. How to cite this article: Li X, Ye S, Lu Y. Long non-coding RNA NEAT1 overexpression associates with increased exacerbation risk, severity, and inflammation, as well as decreased lung function through the interaction with microRNA-124 in asthma.

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Section: Discussionmentioning

confidence: 99%

Longnon‐coding RNA NEAT1 overexpression associates with increased exacerbation risk, severity, and inflammation, as well as decreased lung function through the interaction with microRNA‐124 in asthma

Lü

2019

Clinical Laboratory Analysis

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“…Wang et al ( 2017 ) reported that the differentially expressed lncRNAs induced by DENV-2 infection in hepatic cells revealed that lncRNA may serve as a new diagnostic marker and therapeutic target for DENV-induced liver damage. Additionally, the expression of lncRNA-NEAT1 was reduced in peripheral blood of patients with severe dengue, which is related to the phenotype of severe dengue fever, suggesting that it would be helpful to understand the progress of DENV-induced diseases by monitoring the expression of NEAT1 and IFI27 in peripheral blood (Pandey et al, 2017 ). Therefore, the study of lncRNA contributed to dig out its pathogenic mechanism and explore therapeutic targets in severe dengue.…”

Section: Discussionmentioning

confidence: 99%

ERG-Associated lncRNA (ERGAL) Promotes the Stability and Integrity of Vascular Endothelial Barrier During Dengue Viral Infection via Interaction With miR-183-5p

Zheng

Wang

Cui

et al. 2020

Front. Cell. Infect. Microbiol.

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Dengue virus (DENV) continues to be a major public health problem. DENV infection will cause mild dengue and severe dengue. Severe dengue is clinically manifested as serious complications, including dengue hemorrhagic fever and/or dengue shock syndrome (DHF/DSS), which is mainly characterized by vascular leakage. Currently, the pathogenesis of severe dengue is not elucidated thoroughly, and there are no known therapeutic targets for controlling the disease effectively. This study aimed to further reveal the potential molecular mechanism of severe dengue. In this study, the long non-coding RNA, ERG-associated lncRNA (lncRNA-ERGAL), was activated and significantly up-regulated in DENV-infected vascular endothelial cells. After knockdown of lncRNA-ERGAL, the expression of ERG, VE-cadherin, and claudin-5 was repressed; besides, cell apoptosis was enhanced, and cytoskeletal remodeling was disordered, leading to instability and increased permeability of vascular endothelial barrier during DENV infection. Fluorescence in situ hybridization (FISH) assay showed lncRNA-ERGAL to be mainly expressed in the cytoplasm. Moreover, the expression of miR-183-5p was found to increase during DENV infection and revealed to regulate ERG, junction-associated proteins, and the cytoskeletal structure after overexpression and knockdown. Then, ERGAL was confirmed to interact with miR-183-5p by luciferase reporter assay. Collectively, ERGAL acted as a miRNA sponge that can promote stability and integrity of vascular endothelial barrier during DENV infection via binding to miR-183-5p, thus revealing the potential molecular mechanism of severe dengue and providing a foundation for a promising clinical target in the future.

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“…7 In addition to the importance of TLRs and RIG-I in the immune response, recently increasing evidence has also confirmed the crucial roles of long non-coding RNAs (lncRNAs) profile in modulating host innate immunity during viral infection as well. 8,9 One of the lncRNAs, nuclear-enriched abundant transcript 1(NEAT1), was recently known as a virus-induced lncRNA (VINC) and was considered to be critical for the immune response against virus, however different virus infection, the different expression of NEAT1 observed. [8][9][10] LncRNA NEAT1 expression is upregulated after Hantaan virus (HTNV), HIV-1 infection, and HSV-1 infection, whereas it is downregulated upon Zika virus infection.…”

Section: Hepatitis B Virus (Hbv) Infection Is Still One Of the Biggesmentioning

confidence: 99%

“…8,9 One of the lncRNAs, nuclear-enriched abundant transcript 1(NEAT1), was recently known as a virus-induced lncRNA (VINC) and was considered to be critical for the immune response against virus, however different virus infection, the different expression of NEAT1 observed. [8][9][10] LncRNA NEAT1 expression is upregulated after Hantaan virus (HTNV), HIV-1 infection, and HSV-1 infection, whereas it is downregulated upon Zika virus infection. [8][9][10][11] But the roles of NEAT1 in HBV infection, especially the innate immunity of chronic HBV infection at the active phase, are currently unclear.…”

Section: Hepatitis B Virus (Hbv) Infection Is Still One Of the Biggesmentioning

confidence: 99%

“…[8][9][10] LncRNA NEAT1 expression is upregulated after Hantaan virus (HTNV), HIV-1 infection, and HSV-1 infection, whereas it is downregulated upon Zika virus infection. [8][9][10][11] But the roles of NEAT1 in HBV infection, especially the innate immunity of chronic HBV infection at the active phase, are currently unclear.…”

Section: Hepatitis B Virus (Hbv) Infection Is Still One Of the Biggesmentioning

confidence: 99%

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Toll‐like receptors, long non‐coding RNA NEAT1, and RIG‐I expression are associated with HBeAg‐positive chronic hepatitis B patients in the active phase

Zeng

et al. 2019

Clinical Laboratory Analysis

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BackgroundInnate immunity plays a crucial role in host‐virus interactions and greatly influences viral replication including HBV infection. However, few studies have investigated the possible antiviral immune roles played by TLRs, RIG‐I, and long no‐coding RNA NEAT1 in chronic HBV infection (CHB) patients in clinical samples and their relationships among immune responses. In this study, we sought to investigate the mRNA expression levels of TLR1‐10, RIG‐I, and NEAT1 expression in HBeAg‐positive CHB treatment‐naïve patients with the active phase.MethodsThe expression levels of TLR1‐10, RIG‐I, and NEAT1 of CHB patients with the active phase and healthy controls were measured by qPCR. Serum HBV DNA and routine liver biochemistry including ALT, etc were also measured to evaluate the impaired physiological function of the liver affected by CHB.ResultsThe expression levels of TLR1 and TLR6 in CHB with active phase were remarkably lower than that in healthy controls. The levels of TLR3 in CHB patients with active phase were remarkably higher than that in healthy controls. The total NEAT1 expression was abnormally decreased in CHB patients as compared with healthy controls. The levels of RIG‐I were significantly decreased in CHB patients in the active phase when compared to healthy controls. The expression of TLR6 and RIG‐I was closely correlated with NEAT1 expression. TLR6 level was positively correlated with RIG‐I level.ConclusionChronic HBV infection can alter the innate immune response by downregulating functional expression of TLR1, TLR6, NEAT1.

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Correlation of altered expression of a long non-coding RNA, NEAT1, in peripheral blood mononuclear cells with dengue disease progression

Cited by 26 publications

References 30 publications

Longnon‐coding RNA NEAT1 overexpression associates with increased exacerbation risk, severity, and inflammation, as well as decreased lung function through the interaction with microRNA‐124 in asthma

Longnon‐coding RNA NEAT1 overexpression associates with increased exacerbation risk, severity, and inflammation, as well as decreased lung function through the interaction with microRNA‐124 in asthma

ERG-Associated lncRNA (ERGAL) Promotes the Stability and Integrity of Vascular Endothelial Barrier During Dengue Viral Infection via Interaction With miR-183-5p

Toll‐like receptors, long non‐coding RNA NEAT1, and RIG‐I expression are associated with HBeAg‐positive chronic hepatitis B patients in the active phase

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