Correlation Network Analysis Reveals Relationships between MicroRNAs, Transcription Factor <i>T-bet</i>, and Deregulated Cytokine/Chemokine‐Receptor Network in Pulmonary Sarcoidosis

Dyskova, Tereza; Fillerová, Regina; Novosád, Tomáš; Kudělka, Miloš; Žůrková, Monika; Gajdoš, Petr; Kolek, Vı́tězslav; Kriegová, Eva

doi:10.1155/2015/121378

Cited by 23 publications

(26 citation statements)

References 47 publications

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“…There are some novel findings on top of the results (Table E3), including methylation in the genes for microRNA 202 ( MIR202 ), lipin 1 ( LPIN1 ), and prostaglandin D2 receptor 2 ( PTGDR2 ). MIR202 is differentially expressed in cultured airway epithelial cells of monkeys in response to ozone exposure and lipopolysaccharide (LPS) treatment 26 , and is decreased in bronchoalveolar lavage (BAL) of patients with sarcoidosis 27 . LPIN1 , which has been implicated in lipid metabolism and rhabdomyolisis, encodes a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme.…”

Section: Discussionmentioning

confidence: 99%

An epigenome-wide association study of total serum IgE in Hispanic children

Chen

Wang

Pino-Yanes

et al. 2017

Journal of Allergy and Clinical Immunology

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Background Total immunoglobulin E (IgE) is a therapeutic target in allergic diseases. DNA methylation in white blood cells (WBCs) was associated with total IgE in an epigenome-wide association study (EWAS) of Caucasians. Whether DNA methylation of eosinophils explains those findings is insufficiently understood. Methods We tested for association between genome-wide DNA methylation in WBCs and total IgE in two studies of Hispanic children: the Puerto Rico Genetics of Asthma and Lifestyle Study (PR-GOAL, n = 306) and the Genes-environments and Admixture in Latino Americans (GALA II, n = 573). Whole-genome methylation of DNA from WBCs was measured using the Illumina Infinium HumanMethylation450 BeadChip. Total IgE was measured using the UniCAP 100 system. In PR-GOAL, WBC types (i.e. neutrophils, eosinophils, basophils, lymphocytes, and monocytes) in peripheral blood were measured using Coulter-Counter techniques. In GALA II, WBC types were imputed. Multivariable linear regression was used for the analysis of DNA methylation and total IgE, which was first conducted separately for each cohort, and then combining results from the two cohorts in a meta-analysis. Results CpG sites in multiple genes, including novel findings and results previously reported in Caucasians, were significantly associated with total IgE. However, adjustment for WBC types resulted in markedly fewer significant sites. Top findings from this adjusted meta-analysis were in genes ZFPM1 (P=1.5×10−12), ACOT7 (P=2.5×10−11), and MND1 (P=1.4×10−9). Conclusions In an EWAS adjusted for WBC types (including eosinophils), methylation changes in genes enriched in pathways relevant to asthma and immune responses were associated with total IgE among Hispanic children.

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Section: Discussionmentioning

confidence: 99%

An epigenome-wide association study of total serum IgE in Hispanic children

Chen

Wang

Pino-Yanes

et al. 2017

Journal of Allergy and Clinical Immunology

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“…In addition, some intracellular miRNAs have been reported to have altered expression during sarcoidosis progression [4, 5]. …”

Section: Introductionmentioning

confidence: 99%

The Serum Expression of Selected miRNAs in Pulmonary Sarcoidosis with/without Löfgren’s Syndrome

Novosadová

Chabronova

Kolek

et al. 2016

Mediators of Inflammation

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Purpose. Pulmonary sarcoidosis is associated with dysregulated expression of intracellular miRNAs. There is however only little information on extracellular miRNAs and their association with the disease course in sarcoidosis. We therefore assessed serum miRNAs in sarcoidosis classified according to the presence of Löfgren's syndrome (LS) as a hallmark of good prognosis in contrast to more advanced disease course. Methods. RT-PCR was used to assess 35 miRNAs in 13 healthy controls and 24 sarcoidosis patients (12 with X-ray (CXR) stage ≤ 1 and LS and 12 with insidious onset and CXR stage ≥ 3). Results. Compared to controls, we consistently observed dysregulated expressions of miR-146, miR-16, miR-425-5p, and miR-93-5p in both sarcoidosis groups irrespective of disease course. Specifically, patients without LS had dysregulated expressions of miR-150-5p, miR-1, and miR-212 compared to controls. Patients with LS had dysregulated expressions of miR-21-5p and miR-340-5p compared to controls. Bioinformatics predicted consistently “Pathways in cancer” to be modulated by both altered profiles in patients with/without LS. Three miRNAs (miR-21-5p, miR-340-5p, and miR-212-3p) differed between our patients with LS and those without LS; their cumulative effect may modulate “TGF-β signalling pathway.” Conclusions. Further study should focus on possible applications of serum miRNAs for diagnostics follow-up and for prognosis.

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“…Eight published reports so far have investigated miRNA expression in pulmonary sarcoidosis, but none addressed miRNA encapsulated in EV from the lung and all were limited to cellular expression in blood cells, bronchoalveolar cells and serum; some of these studies, however, provided contradictory results. While some of the previous findings in BAL cells may complement our present observations, the EV/exosomal miRNA remained unexplored in these investigations.…”

Section: Discussionmentioning

confidence: 99%

“…Sarcoidosis is a multisystemic granulomatous disorder of unknown aetiology that mainly affects the lungs. Small non‐coding microRNA (miRNA) are transcriptional regulators and their plausible pro‐inflammatory role in pulmonary sarcoidosis has been suggested . Most studies have investigated the transcription profile of cellular miRNA at the systemic level (peripheral blood cells) .…”

Section: Introductionmentioning

confidence: 99%

“…Most studies have investigated the transcription profile of cellular miRNA at the systemic level (peripheral blood cells) . The association of dysregulated miRNA in bronchoalveolar lavage (BAL) cells and serum from patients with sarcoidosis, its clinical course and its subgroups compared with controls have also been reported . These miRNA are present not only in cellular but also in extracellular compartments .…”

Section: Introductionmentioning

confidence: 99%

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Expression analysis of extracellular microRNA in bronchoalveolar lavage fluid from patients with pulmonary sarcoidosis

et al. 2018

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Our data provide evidence that exosomes and microvesicles as extracellular vesicles are present in the bronchoalveolar space of sarcoidosis patients and they differentially express EV miRNA (miR-146a and miR-150), the expression of which correlates negatively with pulmonary function indices. The significance of these findings for disease pathophysiology and clinical course require further investigation.

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Correlation Network Analysis Reveals Relationships between MicroRNAs, Transcription Factor T-bet, and Deregulated Cytokine/Chemokine‐Receptor Network in Pulmonary Sarcoidosis

Cited by 23 publications

References 47 publications

An epigenome-wide association study of total serum IgE in Hispanic children

An epigenome-wide association study of total serum IgE in Hispanic children

The Serum Expression of Selected miRNAs in Pulmonary Sarcoidosis with/without Löfgren’s Syndrome

Expression analysis of extracellular microRNA in bronchoalveolar lavage fluid from patients with pulmonary sarcoidosis

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