Correlation expression Toll-like receptor 4 with multidrugs resistant tuberculosis in diabetes mellitus condition

Agustin, Heidy; Massi, Muhammad Nasrum; Djaharuddin, Irawati; Susanto, Agus Dwi; Islam, Andi Asadul; Hatta, Mochammad; Bukhari, Agussalim; Bukhari, Agussalim; Santoso, Arif; Burhan, Erlina; Isbaniyah, Fathiyah; Farsida,; Effendy, Zulham

doi:10.1016/j.ijtb.2022.03.012

Cited by 4 publications

(1 citation statement)

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“…The involvement of TLRs leads to the initiation of signaling cascades that ultimately lead to host immune responses. Activation of the TLR signaling pathway can cause the onset of immune diseases ( 61 ). The findings from our study indicated that NECA significantly reduced both the mRNA transcription levels and the protein expression of TLR4 and MYD88 in BMDCs and DCs derived from healthy individuals, as well as in those from patients with DM.…”

Section: Discussionmentioning

confidence: 99%

NECA alleviates inflammatory responses in diabetic retinopathy through dendritic cell toll-like receptor signaling pathway

Li,

Chen,

Wang

et al. 2024

Front. Immunol.

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IntroductionThis study examined the impact of 5’-(N- ethylcarboxamido)adenosine (NECA) in the peripheral blood of healthy individuals, those with diabetes mellitus, diabetic retinopathy (DR), and C57BL/6 mice, both in vivo and in vitro.MethodsEnzyme-linked immunosorbent assay (ELISA) and flow cytometry (FCM) were used to evaluate the effects of NECA on dendritic cells (DCs) and mouse bone marrow-derived dendritic cells (BMDCs) and the effects of NECA-treated DCs on Treg and Th17 cells. The effect of NECA on the Toll-like receptor (TLR) pathway in DCs was evaluated using polymerase chain reaction (PCR) and western blotting (WB).ResultsFCM and ELISA showed that NECA inhibited the expression of surface markers of DCs and BMDCs, increased anti-inflammatory cytokines and decreased proinflammatory cytokines. PCR and WB showed that NCEA decreased mRNA transcription and protein expression in the TLR-4-MyD88-NF-kβ pathway in DCs and BMDCs. The DR severity in streptozocin (STZ) induced diabetic mice was alleviated. NECA-treated DCs and BMDCs were co-cultivated with CD4+T cells, resulting in modulation of Treg and Th17 differentiation, along with cytokine secretion alterations.ConclusionNECA could impair DCs’ ability to present antigens and mitigate the inflammatory response, thereby alleviating the severity of DR.

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Section: Discussionmentioning

confidence: 99%