Correlation Between Viral Load of HBV in Chronic Hepatitis B Patients and Precore and Basal Core Promoter Mutations

Ghabeshi, Soad; Sharifi, Zohreh; Hosseini, Seyed Masoud; Shooshtari, Mahmood Mahmoodian

doi:10.5812/hepatmon.7415

Cited by 21 publications

(16 citation statements)

References 15 publications

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“…www.ajpp.in In present study we observed that the HBV DNA load was high in HBeAg positive cases, and good correlation was seen between the two, but in 26% of HBeAg negative cases the HBV DNA levels were > 20,000 IU/mL which is the level at which treatment is started, monitoring these patients only on basis of HBeAg can be misleading in such cases. Such findings are generally observed in patients with precore mutations (Ghabeshi et al, 2013). Moreover 46 (62.16%) HBeAg negative patients had HBV DNA level more than 29 IU/mL.…”

Section: Resultsmentioning

confidence: 61%

Correlation of hepatitis B virus viral load and HBeAg status in HBsAg positive patients

Nair¹,

Malhotra²,

Pandey³

et al. 2019

Asian J Pharm Pharmacol

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Background: Hepatitis B virus (HBV) infection is a major cause of chronic liver disease and hepatocellular carcinoma. About a one third of the world's population is infected with the HBV. To control the spread of HBV it is important to understand the molecular epidemiology of HBV.Present study was aimed to detect HBV viral Objective: load by Real Time PCR in HBsAg positive patients and to correlate with HBV markers.: A total Material and methods of 100 blood samples were obtained from HBsAg positive patients. DNA was extracted by Roche High Pure System COBAS TaqMan kit and quantified by COBAS TaqMan HBV Test Kit on COBAS TaqMan 48 analyzer (Roche). Results: Majority (48%) of the patients included in the present study were in the age group 20-40 years and most of the patients (60%) were found to be asymptomatic. Out of 100 patients, 80 (80%) were males and only 20 (20%) were females and 28 were HBeAg positive and 72 were HBeAg negative. Of above 28 HBeAg positive samples 16 (57.15%) were having viral load >20000 IU/mL and 12 (42.85%) were having <20000 IU/mL Out of 72 HBeAg negative samples 20 (27.77%) were having viral load >20000 IU/mL and 52 (72.23%) were having <20000 IU/mL. Only 6% (6/100) of the patients, the target was not in detectable (TND) range and these all were HBeAg negative. Conclusion: It's important to carry out HBV viral load testing in patients who are HBsAg positive on regular basis not only in HBeAg positive cases but in negative cases too. As on our study we found HBeAg negative patients had viral load more than 20000 IU/mL which mandates initiation of treatment.

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Section: Resultsmentioning

confidence: 61%

Correlation of hepatitis B virus viral load and HBeAg status in HBsAg positive patients

Nair¹,

Malhotra²,

Pandey³

et al. 2019

Asian J Pharm Pharmacol

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“…The low HBV viral load in our series could explain this- in fact, recent data suggest a significant relationship between high HBV viral load and frequency of PC/BCP mutations in chronic hepatitis B [47]. We tested a small number of patients and this precludes more definitive conclusions.…”

Section: Discussionmentioning

confidence: 66%

Kinetics of Hepatitis B Virus Load During Haemodialysis Sessions and α-Interferon: A Prospective Study

Fabrizi¹,

Lunghi

Alongi³

et al. 2013

Kidney Blood Press Res

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Background: It has been reported a slow progression of hepatitis B in patients undergoing maintenance dialysis, and a role of dialysis session per se has been suggested. The aim of the present study is to evaluate the kinetics of the hepatitis B viral load (HBV DNA) in serum during haemodialysis sessions using a highly sensitive technique; the role of interferon-α in lowering HBV viral load in such patients was also investigated. Methods: HBV DNA was determined in 24 HBsAg positive patients on maintenance hemodialysis immediately before and after a 4-hour hemodialysis session, the same measurements were repeated 48 and 72 hours later. HBV DNA quantitation was performed by a novel RealTime PCR assay. Serum IFN-α levels were tested in parallel in a subset of HD sessions (n=40) by ELISA. Results: 20 (83%) HBsAg positive patients had detectable HBV DNA in serum. Positive status for HBV DNA in serum was not predicted by demographic, clinical or biochemical parameters. HBV load decreased in many patients after hemodialysis sessions 5.92 log₁₀ IU/mL (95% CI, 5.34 to 6.28 log₁₀ IU/mL) vs. 4.79 log₁₀ IU/mL (95% CI, 4.23 to 6.15 log₁₀ IU/mL) (P=0.02). A significant relationship between mean HBV DNA levels before dialysis and percentage reduction of HBV DNA during HD sessions occurred [F-test=5.41, rho (least squares)=0.307]. Increase of serum IFN-α levels was found in a minority (3/40=7%) of HD sessions. Conclusions: Hemodialysis procedure gives reduction of HBV load in HBsAg chronic carriers; no relationship with IFN-α activity during HD sessions was found. The kinetics of HBV viremia in HD procedures could explain the low viral load which is typically observed in these patients. Further studies to identify the mechanisms responsible for reduction of HBV viremia during HD procedures are under way.

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“…Genotype C is prevalent in Asia and the Pacific. Genotype D shows frequently precore mutations and is prevalent in the Mediterranean region and Southern Europe ( 11 ). In patients with HCC in Romania, we detected both core and precore mutations in tissue samples.…”

Section: Discussionmentioning

confidence: 99%

Hepatitis B Virus Core Promoter Mutations in Patients With Chronic Hepatitis B and Hepatocellular Carcinoma in Bucharest, Romania

Constantinescu

Dinu

Boșcaiu³

et al. 2014

Hepat Mon

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Background:Accurate and personalized molecular virological diagnosis of hepatitis B virus (HBV) infection is crucial for individualized selection of patients for antiviral therapy in Romania.Objectives:We aimed to investigate HBV mutations in Romanian patients with chronic HBV infection, also to match HBV genotypes with HBV mutations identified and clinical outcomes.Patients and Methods:This was a cross-sectional study. A total of 484 Romanian patients with chronic HBV infection and hepatocellular carcinoma (HCC) were investigated. This was performed in Fundeni Clinical Institute, Bucharest, Romania during January 2005 to August 2010. HBsAg positive patients with chronic HBV infection admitted to Fundeni Clinical Institute were randomly enrolled in the study. Analysis was performed in the Centre for Immunogenetics and Virology, Fundeni Clinical Institute, Bucharest, Romania. Indirect diagnosis was performed with enhanced chemiluminescence method using Architect i2000SR and HBV-DNA was quantified with COBAS TaqMan HBV PCR. Direct sequencing of the PCR-products was performed with the PCR-product sequencing kit. HBV genotyping was performed with INNO-LiPA DR Amplification and INNO-LiPA HBV precore-core.Results:We detected two HBV genotypes; A (8.1%) and D (60.5%), and a mixture of genotypes A and D (31.4%) (P < 0.001). Basal core promoter (BCP) A1762T/G1764A and precore (PC) G1896A mutations were detected in these Romanian patients with chronic HBV infection. HBV chronic carriers had mainly genotype D (54.4%) and HBV WT (64.0%). BCP A1762T, G1764A and PC G1896A were significantly associated with HCC-tissue HBV sequencing (75.3%) (P < 0.001). PC G1896A alone was detected in HCC-serum HBV sequencing group (66.7%).Conclusions:Genotype D was the main genotype detected in Romanian patients with chronic HBV infection. Genotype D presented both BCP and PC mutations more frequently.

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Correlation Between Viral Load of HBV in Chronic Hepatitis B Patients and Precore and Basal Core Promoter Mutations

Cited by 21 publications

References 15 publications

Correlation of hepatitis B virus viral load and HBeAg status in HBsAg positive patients

Correlation of hepatitis B virus viral load and HBeAg status in HBsAg positive patients

Kinetics of Hepatitis B Virus Load During Haemodialysis Sessions and α-Interferon: A Prospective Study

Hepatitis B Virus Core Promoter Mutations in Patients With Chronic Hepatitis B and Hepatocellular Carcinoma in Bucharest, Romania

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