Correlation between two chemiluminescence based assays for quantification of hepatitis B surface antigen in patients with chronic hepatitis B infection

Gupta, Ekta; Pandey, Priyanka; Kumar, Ashish; Sharma, Mukut; Sarin, S.K.

doi:10.4103/0255-0857.148400

Cited by 8 publications

(3 citation statements)

References 23 publications

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“…Another study by Ozgur et al had reported that there was statistically significant correlation determined between HBsAg level and HBV DNA (P=0.0001)[19]. this also, had concluded by Gupta et al , who found that weak but significant correlation between quantitative HBsAg and HBV-DNA in all the groups (p<0.01)[9].Result in this study revealed that, there is no significant correlation between HBV-DNA levels and histopathology (fibrosis and activity) by Metaver score among the studied groups (p=0.467 & p=0.113 respectively). This finding is supported by other studies.…”

supporting

confidence: 52%

Correlation between HBsAg Quantitation Assay, Histopathology and Viral - DNA Level in Chronic HBV Patients

Mostafa

El-Shewi

El-Raouf

et al. 2015

Afro-Egyptian Journal of Infectious and Endemic Diseases

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Results: A significant correlation between serum HBsAg quantitation level and HBV DNA levels was revealed in all studied HBeAg negative patients (r =0.748, P= <0.001). A significant correlation between serum HBsAg level and fibrosis (r=0.334, P= 0.018). While, there is no significant correlation between serum HBsAg quantitation level and activity by Metaver scoring in all studied patients (P >0.05). Conclusion: All the studied patients were tested for assess correlation between HBsAg quantitation, HBV-DNA level and histopathology grading and staging. There is strong positive correlation between HBsAg quantation level and HBV-DNA and between HBsAg quantation level and stages of fibrosis. No correlation between HBV DNA levels and histopathology.

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supporting

confidence: 52%

Correlation between HBsAg Quantitation Assay, Histopathology and Viral - DNA Level in Chronic HBV Patients

Mostafa

El-Shewi

El-Raouf

et al. 2015

Afro-Egyptian Journal of Infectious and Endemic Diseases

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“…Therefore, the need of the hour is to screen as many individuals as possible, especially in endemic and hyperendemic zones, which are “hotspots” for hepatitis B infection. Although the current chemiluminescent-based qualitative and quantitative HBsAg assays with good sensitivity and specificity are available, 9 , 10 cost and technical expertise make them less accessible in low and middle-income countries. Therefore, accurate and affordable card tests with lower limit-of-detection (LoD) are good alternatives for rapid diagnosis of HBV infection.…”

Section: Discussionmentioning

confidence: 99%

Performance Evaluation of Determine HBsAg 2 Rapid Card Test for the Detection of Hepatitis B Surface Antigen in Clinical Samples

Pandey,

Gupta,

Samal

et al. 2024

Euroasian Journal of Hepato-Gastroenterology

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A bstract Aim and background Hepatitis B virus is one of the leading underlying causes of chronic liver disease. Rapid diagnostic tests with improved sensitivity and specificity for detecting hepatitis B infection could aid in large-scale community screening in resource-limited settings. This study was designed to assess the clinical performance of a rapid card test to detect HBsAg. Materials and methods In this study, archived once-thawed serum samples were tested on the Determine HBsAg 2 card and their performance was evaluated in reference to a chemiluminescence-based assay (HBsAg qualitative assay, Abbott Diagnostics, US). Results A total of 120 patient samples (46 confirmed HBsAg-positive and 74 confirmed HBsAg-negative) were used in this study. The overall median age of the study population was 44 years (IQR: 36–51 years), with a male gender predominance (90%). A specificity of 100% (74/74) and sensitivity of 84.7% (39/46) was observed for the Determine HBsAg 2 assay compared with the reference assay. The samples that showed false-negative results ( n = 7) by the card test had HBsAg levels below the limit-of-detection of the card assay. Conclusion The Determine HBsAg 2 assay gives rapid results in 15 minutes with good sensitivity and specificity. This makes it a good, affordable tool for large-scale screening and public health surveillance programs. Clinical significance Accurate and cost-effective rapid card tests for early detection of Hepatitis B infection would enable quick isolation of infected cases, thus reducing transmission in the community. How to cite this article Samal J, Soni A, Pandey A, et al . Performance Evaluation of Determine HBsAg 2 Rapid Card Test for the Detection of Hepatitis B Surface Antigen in Clinical Samples. Euroasian J Hepato-Gastroenterol 2024;14(1):9–11.

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“…This effect may cause misdetection of HBsAg due to a very high analyte concentration (15). In order to obtain accurate results, during quantification experiments performed with HBsAg II quantification kits (Roche Diagnostics, Mannheim, Germany), 1/400 times dilution of the samples is suggested by the manufacturer (16,17). On the other hand, Zhang et al (14) carried out a study with a quantitative kit (Abbott Diagnostics, Germany) and they have diluted the samples to 1:500 or 1:1000 if they were greater than 250 IU/mL according to the manufacturer's instructions.…”

Section: Discussionmentioning

confidence: 99%

Can HBsAg Be Used as a Viral Replication Marker in Chronic Hepatitis B Patients?

Uzunoğlu¹,

Şahin²,

Avcı³

et al. 2017

vhd

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Correlation between two chemiluminescence based assays for quantification of hepatitis B surface antigen in patients with chronic hepatitis B infection

Abstract: Our study hence proved that both the assays are comparable and a simple qualitative assay with in-house modification can be used easily for quatitation of HBsAg in clinical samples.

Cited by 8 publications

References 23 publications

Correlation between HBsAg Quantitation Assay, Histopathology and Viral - DNA Level in Chronic HBV Patients

Correlation between HBsAg Quantitation Assay, Histopathology and Viral - DNA Level in Chronic HBV Patients

Performance Evaluation of Determine HBsAg 2 Rapid Card Test for the Detection of Hepatitis B Surface Antigen in Clinical Samples

Can HBsAg Be Used as a Viral Replication Marker in Chronic Hepatitis B Patients?

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