Correlation between the spermatozoal characteristics and sperm penetration distance in polacrylamide gel and bovine cervical mucus

Anilkumar, R.; Devanathan, T. G.; Pattabiraman, S. R.; Edwin, M. J.

doi:10.1016/s0093-691x(01)00436-8

Cited by 19 publications

(14 citation statements)

References 9 publications

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“…Cervical mucus substitutes have been used in a number of studies [11,12,26]. Acrylamide has been previously used as a substitute of cervical mucus in other species (human [19,27]; bull [5,20]). Lorton et al [27] noted that bull sperm migration in 1.8% acrylamide was similar to sperm migration in bovine cervical mucus.…”

Section: Discussionmentioning

confidence: 99%

“…These synthetic media were obtained from 30% liquid acrylamide (30% ProtoGel, National Diagnostics, Atlanta, GA, USA), diluting it to the corresponding volume with 1.5 M Tris (pH 8.8) in water. Once mixed, a solution of 2% ammonium persulphate up to 1.5% of final volume and a 0.05% TEMED were added, to induce acrylamide polymerization [5]. The mixture (pH 7.2, 320 mOsm) was left at room temperature for 24 h and then stored at 4°C until use.…”

Section: Preparation Of Synthetic Mediamentioning

confidence: 99%

“…Thus, mucus is considered a means of sperm selection in many species. Taking into account the effect of cervical mucus on sperm transport, the evaluation of the ability of spermatozoa to progress through natural mucus (cervical mucus penetration test [CMPT]) or mucus substitutes has been proposed as an analysis of sperm quality [2][3][4][5][6][7][8][9]. This penetration test has been applied in several animal species and is accepted by the World Health Organization [10] as a means of analyzing human semen.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of ram semen quality using polyacrylamide gel instead of cervical mucus in the sperm penetration test

et al. 2012

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Fertility is a very complex biological function that depends on several properties of the spermatozoa, including sperm motility. Two objectives are analyzed in this study: (1) Replace the cervical mucus by a synthetic medium in a sperm penetration test, and (2) evaluating the results of this test objectively analyzing the sperm number that migrates. In experiment 1, we have tested eight concentrations of acrylamide (1%-2%). Rheological properties of media were analyzed. The plastic straws, loaded with acrylamide, were placed vertically on the semen sample tube for 15 min at 39 °C. After, the acrylamides were placed, by segments of 5 mm, into wells of a 24-well plate, dyed with Hoechst 33342 and the number of spermatozoa were calculated by automated microscopy analysis. The 1.55% and 1.6% acrylamide gel showed a number of spermatozoa emigrating closer to that seen with natural mucus. In experiment 2, we applied the sperm penetration in acrylamide 1.6% and 1.55% using fresh semen and cooled semen at 15 °C and 5 °C. The spermatozoa counts were performed for each segment of 10 mm. Semen chilled at 15 °C presented intermediate values of sperm counts in comparison with fresh semen (higher) and 5 °C chilled semen. The sperm counts do not differ between acrylamides but the rheological properties of acrylamide 1.6% were more similar to those of the natural cervical mucus. In experiment 3, we have observed significant correlations between the number of spermatozoa and several sperm quality parameters (positive: progressive motility and velocity according to the straight path; negative: damaged acrosomes and apoptotic cells) in 1.6% acrylamide media. We conclude that the size of the cell subpopulation, objectively calculated, that migrate beyond 20 mm in 0.5-mL straws filled with acrylamide is a useful parameter in ram sperm quality assessment and further studies are needed to evaluate its relationship with field fertility.

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Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Synthetic Mediamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of ram semen quality using polyacrylamide gel instead of cervical mucus in the sperm penetration test

et al. 2012

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“…Several researchers have elaborated and standardized the human sperm penetration test, substituting hyaluronic acid or acrylamide polymers for cervical mucus [30,31], and the method has also been used to predict outcomes of the zona-free hamster egg penetration test [32]. The technique has also been applied to the assessment of semen in agricultural species, where it distinguished between two groups of bulls classified as "high" or "low" fertility [33,34] and selected both bull and ram spermatozoa on the basis of high progressive motility, high acrosomal and plasma membrane integrity [35] as well as superior sperm head morphology [36]. This approach is a significant departure from studies that have only measured sperm parameters in the bulk sample and tends to imply that the small population of vanguard spermatozoa measured in these methods represents a distinct subpopulation, similar to the oviductal sperm population.…”

Section: Sperm Selection At the Uterotubal Junction And Oviductal Istmentioning

confidence: 99%

Sperm selection in the female mammalian reproductive tract. Focus on the oviduct: Hypotheses, mechanisms, and new opportunities

Holt¹,

Fazeli²

2016

Theriogenology

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“…Intact sperm with proper stimulation and induction of the acrosome reaction are necessary for successful fertilization (28). Anilkumar et al (29) found that there was a positive correlation among the motility, acrosome integrity, and distance traveled by bovine spermatozoa in the artificial mucus or cervical canal. In this study, FITC-PNA was used as the staining method to identify in vitro acrosome integrity in the bulls; however, no statistically significant difference was found among the bulls (P > 0.05).…”

Section: Discussionmentioning

confidence: 99%

The combination of CASA kinetic parameters and fluorescein staining as a fertility tool in cryopreserved bull semen

̇İnanç¹,

Çil²,

Tekin³

et al. 2018

Turk J Vet Anim Sci

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Prediction of male fertility with in vitro semen quality parameters remains a challenge for the bull industry. Fluorescein staining and computer-assisted semen analysis (CASA) ensure kinetically proper and functionally objective results to improve spermatological parameter control. Therefore, we aimed to examine the CASA kinetic parameters and fluorescein staining of cryopreserved bull semen and its relationship with fertility. A total of 90 frozen straws from 5 Simmental bulls were evaluated. After semen was thawed, the motility, kinetic parameters, and insemination doses were evaluated by CASA; fluorescein stainings were evaluated using a fluorescein microscope. The 60th-day nonreturn rates (NRRs) were recorded for determination of pregnancy rates. There were significant differences in motility and kinetic parameters among bulls. Bull 3 had the lowest percentage of NRR and curvilinear velocity, straight line velocity, beat-cross frequency, and lateral head displacement parameters (P < 0.05). A positive correlation was detected between the pregnancy rate and some kinetic parameters. The correlation between the CASA parameters was determined as well. We concluded that various kinetic parameters obtained with CASA software system algorithms and fluorescein stainings can be linked to fertility. However, further research is needed to acquire a more precise link with fertility.

show abstract

Correlation between the spermatozoal characteristics and sperm penetration distance in polacrylamide gel and bovine cervical mucus

Cited by 19 publications

References 9 publications

Evaluation of ram semen quality using polyacrylamide gel instead of cervical mucus in the sperm penetration test

Evaluation of ram semen quality using polyacrylamide gel instead of cervical mucus in the sperm penetration test

Sperm selection in the female mammalian reproductive tract. Focus on the oviduct: Hypotheses, mechanisms, and new opportunities

The combination of CASA kinetic parameters and fluorescein staining as a fertility tool in cryopreserved bull semen

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