Correlation between the release of ovine trophoblast protein-1 by the conceptus and the production of polypeptides by the maternal endometrium of ewes

Sharif, Setareh F.; Francis, Hattie; Keisler, D.H.; Roberts, R. M.

doi:10.1530/jrf.0.0850471

Cited by 18 publications

(10 citation statements)

References 5 publications

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“…[1,2]. Bovine trophoblast protein-1 (bTP-1) is a small (22)(23)(24)(25)(26), acidic (pI = 5.7-6.5) polypeptide that is the major polypeptide shown to be synthesized and secreted in vitro by the bovine conceptus during the critical time of maternal recognition of pregnancy (Days [17][18][19][20] [3,4]. Attachment of the bovine conceptus to the uterine endometrium occurs between Days 18 and 24 of gestation [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…It appears that the actions of IFNs are local, since the receptors for oTP-1 are present in ovine endometrium [16,17] and can be competitively displaced by recombinant bovine IFN-at (rbIFN-a) [18]. Several studies have revealed that oTP-1 can also selectively inhibit or stimulate the synthesis of various endometrial polypeptides in sheep [19,20] that can be mimicked by IFN-a [21].…”

Section: Introductionmentioning

confidence: 99%

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Uterine Cellular Changes in 2′,5′-Oligoadenylate Synthetase during the Bovine Estrous Cycle and Early Pregnancy1

Schmitt

Geisert

Zavy

et al. 1993

Biology of Reproduction

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The signal for maternal recognition of pregnancy in cattle is bovine trophoblast protein-1 (bTP-1), a Type I trophoblast interferon. One of the many functions of interferons is the induction of the 2',5'-oligoadenylate (2-5[A]) system, which is involved in cell division and selective degradation of mRNA. The present study focused on the cellular changes of 2-5(A) synthetase in bovine endometrium during the estrous cycle and early pregnancy. Ability of recombinant bTP-1 to stimulate activity of the enzyme in uterine cells throughout the estrous cycle was also evaluated in vitro. Surface epithelium, glandular epithelium, and stroma were enzymatically separated in cows on Day 5, 10, 15, or 18 postestrus and on Day 15 or 18 of pregnancy. Cell samples were lysed and frozen for determination of the endogenous cellular content of 2-5(A) synthetase. Additional cells obtained during the estrous cycle were cultured and treated with increasing doses of recombinant bovine interferon alpha (rbIFN-alpha) or recombinant bTP-1 (rbTP-1). During the estrous cycle, 2-5(A) synthetase was greatest on Day 5 and declined approximately 10-fold by Day 15 (p < 0.01). Cellular content of 2-5(A) synthetase was similar among all three endometrial cell types. In the gravid horn of pregnant animals, presence of a conceptus significantly increased (p < 0.01) 2-5(A) synthetase in all endometrial cell types compared to levels on Days 15 and 18 of the estrous cycle. On Day 18, levels of 2-5(A) synthetase were 30-fold greater (p < 0.01) in epithelium (surface and glandular) from pregnant cows compared to that from cyclic cows.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Uterine Cellular Changes in 2′,5′-Oligoadenylate Synthetase during the Bovine Estrous Cycle and Early Pregnancy1

Schmitt

Geisert

Zavy

et al. 1993

Biology of Reproduction

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“…Thus, in the current study, the potential existed for IFNs to inhibit secretion of radiolabeled proteins and thereby provide the appearance of enhanced secretion of the remaining protein. Our group [7] and others [22,23] have reported that total secretion of endometrial proteins is not affected by IFN-T treatment or by pregnancy status. This was determined by examining the incorporation of L-[ 3 H]leucine into secreted protein (i.e., specific activity; cpm/pg).…”

Section: Discussionmentioning

confidence: 63%

“…Endometrial explants were cultured in serum-free medium under conditions used by our group [7,8] and others [22][23][24]. Endometrium was collected from cows (n = 5) on Day 18 of pregnancy and cultured in leucine-deficient medium, in the absence or presence of 25 nM rbIFN-T. Cultures were maintained in an atmosphere of 50% 02:45% N 2 :5% CO 2 at 37°C to characterize timing of secretion of the uterine proteins.…”

Section: Timing Of Secretion Of Endometrial Proteinsmentioning

confidence: 99%

Secretion of Bovine Uterine Proteins in Response to Type I Interferons1

Naivar

Ward

Austin

et al. 1995

Biology of Reproduction

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Bovine interferon-tau (bIFN-tau) is secreted by the developing conceptus and initiates antiluteolytic events by interacting with uterine membrane receptors. We have identified three endometrial proteins (approximately 8, 16, and 28 kDa; P8, P16, and P28; respectively) that are secreted in response to recombinant (r) bIFN-tau. The objective of this study was to determine whether or not secretion of these proteins was a unique response to IFN-tau during early pregnancy. Three experiments were designed to examine secretion of endometrial proteins as a function of time in culture (0, 3, 6, 12, 18, 24 h), stage of the estrous cycle and pregnancy (Days 15, 18, 0/21), and dose of Type I IFN (0, 0.5, 5, and 25 nM; rbIFN-tau, rbIFN-alpha, and roIFN-tau). Endometrium was cultured for times specified with L-[3H]leusine to generate radiolabeled proteins. Secreted proteins were quantitated by using one-dimensional (1D)-PAGE, fluorography, and densitometry. Secretion of P8, P16, and P28 increased over time (p < 0.0001) in culture and in response to 25 nM rbIFN-tau (p < 0.05). Secretion of P8 in response to rbIFN-tau was higher (p < 0.05). Secretion of P8 in response to rbIFN-tau was higher (p < 0.0005) in endometrium collected from pregnant than nonpregnant heifers, but did not differ across the days examined. Although secretion of P8 was higher (p < 0.0001) in the presence than in the absence of rbIFN-tau, it was not affected by rbIFN-alpha.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…The unilateral pregnant sheep model (Moor and Rowson, 1966) enables changes in the expression of endometrial genes in the presence of conceptuses to be studied during early pregnancy (Sharif et al, 1989;Sandra et al, 2005). In sheep, cellular contact between conceptus trophectoderm and epithelium of the caruncular areas occurs on day 16 of post-mating, a dynamic process completed by day 22 of pregnancy (Spencer et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

The conceptus regulates tryptophanyl-tRNA synthetase and superoxide dismutase 2 in the sheep caruncular endometrium during early pregnancy

Al-Gubory

Arianmanesh

Garrel

et al. 2015

The International Journal of Biochemistry & Cell Biology

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Conceptus-derived paracrine signals play crucial roles in the preparation of a uterine environment capable of supporting implantation and development of the conceptus. However, little is known about the regulation of endometrial tryptophanyl tRNA synthetase (WARS) and manganese superoxide dismutase (SOD2) protein expression by the implanting and post-implanting conceptus. We hypothesized that the conceptus-derived signals favourably influences uterine environment for implantation through regulation of WARS and SOD2 expression in ovine caruncular endometrium. To test this hypothesis, WARS and SOD2 protein and mRNA expression was determined in caruncular endometrial tissues of unilaterally pregnant ewes at implantation (day 16) and post-implantation (day 20) periods. WARS protein expression increased in caruncular tissues of the gravid uterine horns compared with the non-gravid uterine horns on days 16 and 20 of pregnancy. There were no changes in SOD2 protein expression between the gravid and non-gravid uterine horns, irrespective of the day of pregnancy. On day 16 of pregnancy, there were no differences in WARS and SOD2 mRNA expression between the gravid and non-gravid uterine horns but expression of both genes was higher in the gravid uterine horns when compared with the non-gravid uterine horns on day 20 of pregnancy. In conclusion, the use of the unilaterally pregnant ewe model provides for the first time firm evidence that the early implantation and post-implanting conceptus-derived signals up-regulate WARS protein expression within the caruncular endometrium. Further studies are necessary to identify these signalling molecules and to understand mechanisms whereby they exert paracrine action within the endometrium.

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Correlation between the release of ovine trophoblast protein-1 by the conceptus and the production of polypeptides by the maternal endometrium of ewes

Cited by 18 publications

References 5 publications

Uterine Cellular Changes in 2′,5′-Oligoadenylate Synthetase during the Bovine Estrous Cycle and Early Pregnancy1

Uterine Cellular Changes in 2′,5′-Oligoadenylate Synthetase during the Bovine Estrous Cycle and Early Pregnancy1

Secretion of Bovine Uterine Proteins in Response to Type I Interferons1

The conceptus regulates tryptophanyl-tRNA synthetase and superoxide dismutase 2 in the sheep caruncular endometrium during early pregnancy

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