Correlation between the regulation of intestinal bacteriophages by green tea polyphenols and the flora diversity in SPF mice

Fang, Xiang; Dong, Sashuang; Xin, Zilong; He, Wencan; Yu-ling, Zhang; Xiong, Jiaqi; Wang, Jie; Liao, Zhenlin; Wang, Li; Qin, Zhong; Wei, Hong

doi:10.1039/d1fo03694g

Cited by 14 publications

(7 citation statements)

References 74 publications

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“…As a prokaryotic virus, the phage is usually studied by viral metagenomics (Huang D. et al, 2021;Dong et al, 2022). In this study, the dominant viral families in shrimp intestine were Microviridae, Circoviridae, Inoviridae, Siphoviridae, Podoviridae, and Myoviridae, which were also identified in the intestine of fish, duck, and mosquito (Fawaz et al, 2016;Filipa-Silva et al, 2020;Nebbak et al, 2021).…”

Section: Discussionmentioning

confidence: 62%

Phage-prokaryote coexistence strategy mediates microbial community diversity in the intestine and sediment microhabitats of shrimp culture pond ecosystem

Deng

Zeng²,

Zhou³

et al. 2022

Front. Microbiol.

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Emerging evidence supports that the phage-prokaryote interaction drives ecological processes in various environments with different phage life strategies. However, the knowledge of phage-prokaryote interaction in the shrimp culture pond ecosystem (SCPE) is still limited. Here, the viral and prokaryotic community profiles at four culture stages in the intestine of Litopenaeus vannamei and cultural sediment microhabitats of SCPE were explored to elucidate the contribution of phage-prokaryote interaction in modulating microbial communities. The results demonstrated that the most abundant viral families in the shrimp intestine and sediment were Microviridae, Circoviridae, Inoviridae, Siphoviridae, Podoviridae, Myoviridae, Parvoviridae, Herelleviridae, Mimiviridae, and Genomoviridae, while phages dominated the viral community. The dominant prokaryotic genera were Vibrio, Formosa, Aurantisolimonas, and Shewanella in the shrimp intestine, and Formosa, Aurantisolimonas, Algoriphagus, and Flavobacterium in the sediment. The viral and prokaryotic composition of the shrimp intestine and sediment were significantly different at four culture stages, and the phage communities were closely related to the prokaryotic communities. Moreover, the phage-prokaryote interactions can directly or indirectly modulate the microbial community composition and function, including auxiliary metabolic genes and closed toxin genes. The interactional analysis revealed that phages and prokaryotes had diverse coexistence strategies in the shrimp intestine and sediment microhabitats of SCPE. Collectively, our findings characterized the composition of viral communities in the shrimp intestine and cultural sediment and revealed the distinct pattern of phage-prokaryote interaction in modulating microbial community diversity, which expanded our cognization of the phage-prokaryote coexistence strategy in aquatic ecosystems from the microecological perspective and provided theoretical support for microecological prevention and control of shrimp culture health management.

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Section: Discussionmentioning

confidence: 62%

Phage-prokaryote coexistence strategy mediates microbial community diversity in the intestine and sediment microhabitats of shrimp culture pond ecosystem

Deng

Zeng²,

Zhou³

et al. 2022

Front. Microbiol.

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“…Alpha-diversity indices, Chao1 and Simpson, were applied to assess species richness and diversity, reflecting variations in microbial diversity and abundance across different samples. 26 Fig. 6A shows that the Chao1 value of the FOS group was significantly lower ( p < 0.05) than those of the other groups after 48 h of in vitro fermentation.…”

Section: Resultsmentioning

confidence: 86%

“…6C) comparing the microbial community composition among samples was conducted based on the Bray-Curtis distance index. 26 The results revealed statistically significant differences (ANOSIM, R = 0.974, p = 0.001) between all the groups, with the bacterial community of samples from the Boletus polysaccharide groups being more closely clustered with each other than with the controls.…”

Section: Papermentioning

confidence: 91%

Structural characteristics and in vitro fermentation patterns of polysaccharides from Boletus mushrooms

Chen¹,

Wang²,

Liu³

et al. 2023

Food Funct.

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“…Spearman's rank correlations and their significances were calculated using the cor and cor.test functions in R, respectively (25). The correlation (r-value) was calculated and is shown in yellow to blue, representing positive and negative correlations, respectively (Figure 1).…”

Section: Correlation Analysismentioning

confidence: 99%

Metagenomic and metabolomic analyses show correlations between intestinal microbiome diversity and microbiome metabolites in ob/ob and ApoE−/− mice

Dong

et al. 2022

Front. Nutr.

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Obesity and atherosclerosis are the most prevalent metabolic diseases. ApoE−/− and ob/ob mice are widely used as models to study the pathogenesis of these diseases. However, how gut microbes, gut bacteriophages, and metabolites change in these two disease models is unclear. Here, we used wild-type C57BL/6J (Wt) mice as normal controls to analyze the intestinal archaea, bacteria, bacteriophages, and microbial metabolites of ob/ob and ApoE−/− mice through metagenomics and metabolomics. Analysis of the intestinal archaea showed that the abundances of Methanobrevibacter and Halolamina were significantly increased and decreased, respectively, in the ob/ob group compared with those in the Wt and ApoE−/− groups (p < 0.05). Compared with those of the Wt group, the relative abundances of the bacterial genera Enterorhabdus, Alistipes, Bacteroides, Prevotella, Rikenella, Barnesiella, Porphyromonas, Riemerella, and Bifidobacterium were significantly decreased (p < 0.05) in the ob/ob mice, and the relative abundance of Akkermansia was significantly decreased in the ApoE−/− group. The relative abundances of A. muciniphila and L. murinus were significantly decreased and increased, respectively, in the ob/ob and ApoE−/− groups compared with those of the Wt group (p < 0.05). Lactobacillus_ prophage_ Lj965 and Lactobacillus _ prophage _ Lj771 were significantly more abundant in the ob/ob mice than in the Wt mice. Analysis of the aminoacyl-tRNA biosynthesis metabolic pathway revealed that the enriched compounds of phenylalanine, glutamine, glycine, serine, methionine, valine, alanine, lysine, isoleucine, leucine, threonine, tryptophan, and tyrosine were downregulated in the ApoE−/− mice compared with those of the ob/ob mice. Aminoacyl-tRNA synthetases are considered manifestations of metabolic diseases and are closely associated with obesity, atherosclerosis, and type 2 diabetes. These data offer new insight regarding possible causes of these diseases and provide a foundation for studying the regulation of various food nutrients in metabolic disease models.

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Correlation between the regulation of intestinal bacteriophages by green tea polyphenols and the flora diversity in SPF mice

Abstract: Green tea polyphenols (GTP) play an important role in shaping the gut microbiome, comprising of a range of densely colonizing microorganisms, including bacteriophages. Previous studies focused on the effect of...

Cited by 14 publications

References 74 publications

Phage-prokaryote coexistence strategy mediates microbial community diversity in the intestine and sediment microhabitats of shrimp culture pond ecosystem

Phage-prokaryote coexistence strategy mediates microbial community diversity in the intestine and sediment microhabitats of shrimp culture pond ecosystem

Structural characteristics and in vitro fermentation patterns of polysaccharides from Boletus mushrooms

Metagenomic and metabolomic analyses show correlations between intestinal microbiome diversity and microbiome metabolites in ob/ob and ApoE−/− mice

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